Description: A process of global importance in carbon cycling is the remineralization of algae biomass by heterotrophic bacteria, most notably during massive marine algae blooms. Such blooms can trigger secondary blooms of planktonic bacteria that consist of swift successions of distinct bacterial clades, most prominently members of the Flavobacteriia, Gammaproteobacteria and the alphaproteobacterial Roseobacter clade. This study explores such successions during spring phytoplankton blooms in the southern North Sea (German Bight) for four consecutive years. The surface water samples were taken at Helgoland Island about 40 km offshore in the southeastern North Sea in the German Bight at the station 'Kabeltonne' (54° 11.3' N, 7° 54.0' E) between the main island and the minor island, Düne (German for 'dune') using small research vessels (http://www.awi.de/en/expedition/ships/more-ships.html). Water depths at this site fluctuate from 6 to 10 m over the tidal cycle. Samples were processed as described previously (Teeling et al., 2012; doi:10.7554/eLife.11888.001) in the laboratory of the Biological Station Helgoland within less than two hours after sampling. Assessment of absolute cell numbers and bacterioplankton community composition was carried out as described previously (Thiele et al., 2011; doi:10.1016/B978-0-444-53199-5.00056-7). To obtain total cell numbers, DNA of formaldehyde fixed cells filtered on 0.2 mm pore sized filters was stained with 4',6-diamidino-2-phenylindole (DAPI). Fluorescently labeled cells were subsequently counted on filter sections using an epifluores-cence microscope. Likewise, bacterioplankton community composition was assessed by catalyzedreporter deposition fluorescence in situ hybridization (CARD-FISH) of formaldehyde fixed cells on 0.2 mm pore sized filters.
Global identifier:
Doi(
"10.1594/PANGAEA.860256",
)
DataMeasurements(
DataMeasurements {
domain: Unspecified,
station: None,
measured_variables: [
"Actinobacteria, targeted with HGC69a oligonucleotide FISH-probe",
"Alphaproteobacteria, targeted with ALF968 oligonucleotide FISH-probe",
"Alteromonas/Colwellia, targeted with Alt1413 oligonucleotide FISH-probe",
"Archaea, targeted with ARCH915 oligonucleotide FISH-probe",
"Bacteria, targeted with EUB338(I-III) oligonucleotide FISH-probe",
"Bacterioplankton",
"Balneatrix, targeted with Bal731 oligonucleotide FISH-probe",
"Betaproteobacteria, targeted with BET42a oligonucleotide FISH-probe",
"Crenarchaeota marine group I, targeted with Cren554 oligonucleotide FISH-probe",
"Cytophaga-Flavobacterium cluster, targeted with CF319a oligonucleotide FISH-probe",
"DATE/TIME",
"DEPTH, water",
"Euryarchaeota marine group II, targeted with Eury806 oligonucleotide FISH-probe",
"Formosa, targeted with FORM181A oligonucleotide FISH-probe",
"Formosa, targeted with FORM181B oligonucleotide FISH-probe",
"Gammaproteobacteria, targeted with Gam42a oligonucleotide FISH-probe",
"Glaciecola, targeted with Glac227 oligonucleotide FISH-probe",
"Julian day",
"Marinoscillum, targeted with CYT-734 oligonucleotide FISH-probe",
"NAC11-7 clade, targeted with NAC11-7-1030 oligonucleotide FISH-probe",
"NOR5 clade, targeted with NOR5-730 oligonucleotide FISH-probe",
"NS3a clade, targeted with NS3a-840 oligonucleotide FISH-probe",
"NS5 clade, targeted with NS5/DE2-471 oligonucleotide FISH-probe",
"NS5 clade, targeted with NS5/VIS1-575 oligonucleotide FISH-probe",
"NS9 clade, targeted with NS9-664 oligonucleotide FISH-probe",
"Non-bacteria control, targeted with NON338 oligonucleotide FISH-probe",
"OM182 clade, targeted with OM182-707 oligonucleotide FISH-probe",
"Pirellula group D, targeted with PirD1039 oligonucleotide FISH-probe",
"Planctomyce group A, targeted with PlaA1228 oligonucleotide FISH-probe",
"Planctomycete group B, targeted with uPlaB440 oligonucleotide FISH-probe",
"Planctomycetes, targeted with Pla46 oligonucleotide FISH-probe",
"Polaribacter, targeted with POL740 oligonucleotide FISH-probe",
"Pseudoalteromonas, targeted with PSA184 oligonucleotide FISH-probe",
"Reinekea, targeted with REI731 oligonucleotide FISH-probe",
"Roseobacter clade, targeted with RCA1000 oligonucleotide FISH-probe",
"Roseobacter clade, targeted with ROS537 oligonucleotide FISH-probe",
"SAR11 clade, targeted with SAR11-441 oligonucleotide FISH-probe",
"SAR11 clade, targeted with SAR11-486 oligonucleotide FISH-probe",
"SAR324 clade, targeted with SAR324-1412 oligonucleotide FISH-probe",
"SAR86 clade, targeted with SAR86-1245 oligonucleotide FISH-probe",
"SAR92 clade, targeted with SAR92-627 oligonucleotide FISH-probe",
"Ulvibacter, targeted with ULV995 oligonucleotide FISH-probe",
"VIS6 clade, targeted with VIS6-814 oligonucleotide FISH-probe",
"Vibrio, targeted with GV841 oligonucleotide FISH-probe",
],
methods: [
"Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH)",
"Epifluorescence microscopy after DAPI staining",
],
},
)
Tags: Algenblüte ? Kohlenstoff ? Formaldehyd ? Kind ? Bakterien ? Deposition ? Insel ? Algenbiomasse ? Oberflächengewässer ? Studie ? Wasser ? Deutsche Bucht ? Forschungsschiff ? Zeitreihe ? Offshore ? Nordsee ?
Region: German Bight, North Sea
Bounding boxes: 7.9° .. 7.9° x 54.188° .. 54.188°
License: Creative Commons Namensnennung 3.0
Language: Englisch/English
Issued: 2016-05-09
Modified: 2026-09-03
Last harvest: 13.10.2026 03:09
Time ranges: 2009-01-05 - 2012-06-20
Accessed 2 times.