Description: Ocean acidification causes an accumulation of CO2 in marine organisms and leads to shifts in acid-base parameters. Acid-base regulation in gill breathers involves a net increase of internal bicarbonate levels through transmembrane ion exchange with the surrounding water. Successful maintenance of body fluid pH depends on the functional capacity of ion-exchange mechanisms and associated energy budget. For a detailed understanding of the dependence of acid-base regulation on water parameters, we investigated the physiological responses of the shore crab Carcinus maenas to 4 weeks of ocean acidification [OA, P(CO2)w = 1800 µatm], at variable water bicarbonate levels, paralleled by changes in water pH. Cardiovascular performance was determined together with extra-(pHe) and intracellular pH (pHi), oxygen consumption, haemolymph CO2 parameters, and ion composition. High water P(CO2) caused haemolymph P(CO2) to rise, but pHe and pHi remained constant due to increased haemolymph and cellular [HCO3-]. This process was effective even under reduced seawater pH and bicarbonate concentrations. While extracellular cation concentrations increased throughout, anion levels remained constant or decreased. Despite similar levels of haemolymph pH and ion concentrations under OA, metabolic rates, and haemolymph flow were significantly depressed by 40 and 30%, respectively, when OA was combined with reduced seawater [HCO3-] and pH. Our findings suggest an influence of water bicarbonate levels on metabolic rates as well as on correlations between blood flow and pHe. This previously unknown phenomenon should direct attention to pathways of acid-base regulation and their potential feedback on whole-animal energy demand, in relation with changing seawater carbonate parameters.
Global identifier:
Doi(
"10.1594/PANGAEA.892815",
)
Measurements {
domain: Unspecified,
station: None,
measured_variables: [
"Type",
"Species",
"Registration number of species",
"Uniform resource locator/link to reference",
"Experiment duration",
"Treatment",
"Identification",
"Wet mass",
"Volume",
"Metabolic rate, standard",
"Metabolic rate of oxygen, standard",
"Difference",
"Factorial aerobic scope",
"pH, intracellular",
"pH, extracellular",
"Heart rate",
"Flow rate",
"Haemolymph, total carbon dioxide",
"Haemolymph, partial pressure of carbon dioxide",
"Haemolymph, bicarbonate ion",
"Chloride",
"Sodium ion",
"Potassium ion",
"Magnesium ion",
"Calcium ion",
"Temperature, water",
"Temperature, water, standard deviation",
"Salinity",
"Salinity, standard deviation",
"Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)",
"Partial pressure of carbon dioxide, standard deviation",
"Bicarbonate",
"Bicarbonate, standard deviation",
"pH, free scale",
"pH, standard deviation",
"Hydrogen ion",
"Hydrogen ion, standard deviation",
"Alkalinity, total",
"Alkalinity, total, standard deviation",
"Carbon, inorganic, dissolved",
"Carbon, inorganic, dissolved, standard deviation",
"Carbonate system computation flag",
"pH, total scale",
"Carbon dioxide",
"Fugacity of carbon dioxide (water) at sea surface temperature (wet air)",
"Bicarbonate ion",
"Carbonate ion",
"Carbon, inorganic, dissolved",
"Alkalinity, total",
"Aragonite saturation state",
"Calcite saturation state",
],
methods: [
"Calculated using seacarb after Nisumaa et al. (2010)",
"Calculated using seacarb after Nisumaa et al. (2010)",
"Calculated using seacarb after Nisumaa et al. (2010)",
"Calculated using seacarb after Nisumaa et al. (2010)",
"Calculated using seacarb after Nisumaa et al. (2010)",
"Calculated using seacarb after Nisumaa et al. (2010)",
"Calculated using seacarb after Nisumaa et al. (2010)",
"Calculated using seacarb after Nisumaa et al. (2010)",
"Calculated using seacarb after Nisumaa et al. (2010)",
"Calculated using seacarb after Nisumaa et al. (2010)",
],
}
Dataset
Comment: In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2018-08-06.
Origins: /Wissenschaft/PANGAEA
Tags: Arthropoden ? Meerestemperatur ? Meerwasser ? Wasserstoff ? Wassertemperatur ? Benthos ? Gesamtkohlenstoff ? Kohlendioxid ? Meeresorganismen ? Salzgehalt ? Ozeanversauerung ? Atmung ? Blut ? Energie ? Energiebedarf ? Fließgeschwindigkeit ? Wasserströmung ? Ionenaustausch ? Laborversuch ? Wasser ? Nordatlantik ? Ressource ? Acid-base regulation ? Animalia ? Behaviour ? Benthic animals ? Carcinus maenas ? Coast and continental shelf ? Containers and aquaria (20-1000 L or < 1 m**2) ? Single species ? Temperate ?
Bounding boxes: 7.74325° .. 7.74325° x 53.74106° .. 53.74106°
License: cc-by/3.0
Language: Englisch/English
Issued: 2018-08-06
Modified: 2022-12-15
Time ranges: 2014-10-01 - 2014-10-31
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