Description: We investigated effects of temperature during ontogeny of the kelp Laminaria digitata across haploid gametophyte and diploid sporophyte life cycle stages in 5 distinct genetic lines. We sampled meristematic discs from wild sporophytes on the island of Helgoland (North Sea; 54.1779 N, 7.8926 E) in May 2017, July 2017 and February 2018 and conducted a seasonal growth experiment at 5°C and 15°C over 14 days (experiment 1). Based on meiospores from five individuals (genetic lines) sampled in July 2017, we applied a full-factorial experimental design to generate different temperature histories by applying 5°C and 15°C during meiospore germination, gametogenesis of parental gametophytes and recruitment of offspring sporophytes (19-26 days; recruitment), and juvenile sporophyte rearing (91-122 days). We then tested for thermal plasticity among temperature history treatments at 5°C and 15°C in a final 12-day experiment assessing growth, the storage compound mannitol, carbon and nitrogen contents, and fluorometric responses in 3-4 month old sporophytes (experiment 2).
Global identifier:
Doi(
"10.1594/PANGAEA.917810",
)
Dataset
Origins: /Wissenschaft/PANGAEA
Tags: Stickstoffgehalt ? Genetische Variation ? Wärmespeicher ? Insel ? Züchtung ? Nordsee ? biphasic life cycle ? carry-over effect ? developmental plasticity ? gametogenesis ? ontogeny ? phenotypic plasticity ? temperature acclimation ?
Bounding boxes: 7.8926° .. 7.8926° x 54.1779° .. 54.1779°
License: cc-by/4.0
Language: Englisch/English
Issued: 2020-05-25
Modified: 2020-05-26
Time ranges: 2017-05-10 - 2018-02-04
Accessed 1 times.