Description: Sandy streambed sediments that had been exposed to either intense drying (90-day without rainfall) or bedform migration (periodically moving ripples) were placed in 250 µm mesh bags to the Spree River in Northeastern Germany on October 22, 2018, to initiate the recovery process from drying or migration stress. Subsamples from the recovering sediments were collected at eight dates within eight months and taken to the laboratory for further analysis. Community abundance was assessed on a heterotrophic (amplified bacterial and fungal gene copies) and on an autotrophic level (Chlorophyll a concentration and diatom cell count). The aim of the experiment was to observe the long-term recovery of microbial communities in lowland streams from drying and bedform migration and to identify if stress effects persisted as legacy after the stress period.
Global identifier:
Doi(
"10.1594/PANGAEA.941189",
)
DataMeasurements(
DataMeasurements {
domain: Unspecified,
station: None,
measured_variables: [
"Chlorophyll a, per unit sediment mass",
"DATE/TIME",
"Diatoms",
"Fungal abundance, ITS2 copy number per unit sediment dry mass",
"Prokaryotic abundance, 16S gene copy number per unit sediment dry mass",
"Time in days",
"Treatment",
],
methods: [
"Counting 400 living cells in Utermöhl chamber at 400x magnification, inverse light microscope (Axioskop2, Zeiss, Germany), extraction following Mendoza-Lera et al. (2017)",
"Reverse phase HPLC (High Performance Liquid Chromatography) using Ultimate3000 (Thermo Fisher Scientific Corporation, Waltham, MA, USA)",
"qPCR (primer pairs 27F/519r) with SYBR™ Green in ABI7500 machine (Applied Biosystems, Foster City, USA), Perez-Mon et al. (2020)",
"qPCR (primer pairs ITS3/ITS4) with SYBR™ Green in ABI7500 machine (Applied Biosystems, Foster City, USA), Perez-Mon et al. (2020)",
],
},
)
Tags: Fluss ? Deutschland ? Dürre ? Sediment ? Sedimenttransport ? Niederschlag ? microbial abundance ? microbial community ? ripples ? streambed metabolism ?
Region: Döbbrick
Bounding boxes: 14.333333° .. 14.333333° x 51.833333° .. 51.833333° 14.3406° .. 14.3406° x 51.81947° .. 51.81947°
License: cc-by/4.0
Language: Englisch/English
Issued: 2023-04-26
Modified: 2023-04-26
Time ranges: 2018-10-22 - 2019-06-03
Accessed 1 times.