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Enzyme activity after drying and bedform migration in the Spree River in 2018/2019

Description: Sandy streambed sediments that had been exposed to either intense drying (90-day without rainfall) or bedform migration (periodically moving ripples) were placed in 250 µm mesh bags to the Spree River in Northeastern Germany on October 22, 2018, to initiate the recovery process from drying or migration stress. Subsamples from the recovering sediments were collected at eight dates within eight months and taken to the laboratory for further analysis. Potential extracellular enzyme activities (Beta-Glucosidase, Xylosidase, Chitinase, Phosphatase, Leucine-Aminopeptidase, Phenol-Oxidase, and Peroxidase) were assessed by measuring fluorescence and absorbance after extraction from sediments. The aim of the experiment was to observe the long-term recovery of microbial communities in lowland streams from drying and bedform migration and to identify if stress effects persisted as legacy after the stress period.

Global identifier:

Doi(
    "10.1594/PANGAEA.941234",
)

Types:
DataMeasurements(
    DataMeasurements {
        domain: Unspecified,
        station: None,
        measured_variables: [
            "Beta-glucosidase activity",
            "Chitinase activity",
            "DATE/TIME",
            "Leucine aminopeptidase activity",
            "Peroxidase activity",
            "Phenol oxidase activity",
            "Phosphatase activity",
            "Time in days",
            "Treatment",
            "Xylosidase activity",
        ],
        methods: [
            "AMC, Infinite 200 fluorometer (Tecan, Männedorf, Switzerland), 365/450\u{a0}nm (excitation/emmission wavelength), standardized to 20°C, Q10 factor of 2 (Winkler et al. 1996)",
            "L-DOPA, 200 fluorometer (Tecan, Männedorf, Switzerland), 365/460\u{a0}nm (excitation/emmission wavelength), standardized to 20°C, Q10 factor of 2 (Winkler et al. 1996)",
            "MUB, Infinite 200 fluorometer (Tecan, Männedorf, Switzerland), 365/445\u{a0}nm (excitation/emmission wavelength), standardized to 20°C, Q10 factor of 2 (Winkler et al. 1996)",
        ],
    },
)

Tags: Phenol ? Fluss ? Deutschland ? Dürre ? Sediment ? Sedimenttransport ? Niederschlag ? microbial abundance ? microbial community ? ripples ? streambed metabolism ?

Region: Döbbrick

Bounding boxes: 14.333333° .. 14.333333° x 51.833333° .. 51.833333° 14.3406° .. 14.3406° x 51.81947° .. 51.81947°

License: cc-by/4.0

Language: Englisch/English

Organisations

Persons

Issued: 2023-04-26

Modified: 2023-04-26

Time ranges: 2018-10-22 - 2019-02-04

Resources

Status

Quality score

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