Description: This dataset comprises the microbial community composition of biological soil crusts in north-German sand dunes. For this we obtained enrichment cultures of phototrophic microorganisms, by placing fragments of biocrusts of the same Petri dishes as used for sequencing, in Petri dishes with Bold Basal (1N BBM) agarized medium (Bischoff and Bold 1963). Cultures were grown under standard laboratory conditions: with a 12-hour alteration of light and dark phases and irradiation of 25 μmol photons m-2 s-1 at a temperature 20 ± 5 ºС. Microscopic study of these raw cultures began in the third week of cultivation. Morphological examinations were performed using Olympus BX53 light microscope with Nomarski DIC optics (Olympus Ltd, Hamburg, Germany). Micrographs were taken with a digital camera (Olympus LC30) attached to the microscope, and processed by the Olympus software cellSens Entry. Direct microscopy of rewetted samples was performed in parallel with cultivation for evaluation of dominating species of algae and cyanobacteria in the original samples. Morphological identification of the biocrust organisms was based mainly on Ettl and Gärtner (2014) for green microalgae, and on Komárek (2013) for cyanobacteria, as well as on some monographs and papers devoted to taxonomic revisions of the taxa of interest (Darienko and Pröschold 2019). Moss and lichens samples were air-dried after collection. For determination, a microscope with a maximum magnification of 400x was used. Morphological identification of mosses followed Frahm and Frey (2004) with taxonomical reference to (Hodgetts et al. 2020). Lichens were determined according to Wirth et al. (2013). Morphologically critical species of the genus Cladonia where additionally analyzed by thin-layer chromatography according to (Culberson and Ammann 1979) in solvent system A.
Global identifier:
Doi(
"10.1594/PANGAEA.947829",
)
Measurements {
domain: Unspecified,
station: None,
measured_variables: [
"Event label",
"DATE/TIME",
"Latitude of event",
"Longitude of event",
"Elevation of event",
"Location",
"Site",
"Actinochloris terrestris",
"Amandinea punctata",
"Asterococcus sp.",
"Bacidina etayana",
"Brachythecium albicans",
"Bracteacoccus sp.",
"Bryum capillare",
"Caloplaca cerinella",
"Campylopus introflexus",
"Cephaloziella divaricata",
"Ceratodon purpureus",
"Chlorella vulgaris",
"Chlorococcum sp.",
"Chloroidium cf. ellipsoideum",
"Chlorokybus atmophyticus",
"Chlorolobion sp.",
"Cladonia arbuscula",
"Cladonia chlorophaea",
"Cladonia ciliata",
"Cladonia coccifera",
"Cladonia conista",
"Cladonia fimbriata",
"Cladonia floerkeana",
"Cladonia foliacea",
"Cladonia furcata",
"Cladonia glauca",
"Cladonia gracilis",
"Cladonia humilis",
"Cladonia macilenta",
"Cladonia novochlorophaea",
"Cladonia phyllophora",
"Cladonia portentosa",
"Cladonia ramulosa",
"Cladonia rangiformis",
"Cladonia rei",
"Cladonia scabriuscula",
"Cladonia subulata",
"Cladonia uncialis ssp. biuncialis",
"Coccomyxa sp.",
"Coelastrella sp.",
"Cylindrocystis cf. crassa",
"Cylindrocystis sp.",
"Dicranum scoparium",
"Diplosphaera chodatii",
"Elliptochloris subsphaerica",
"Eremochloris sp.",
"Evernia prunastri",
"Hennediella heimii",
"Hypnum cupressiforme",
"Hypnum cupressiforme var. lacunosum",
"Hypnum jutlandicum",
"Hypogymnia physodes",
"Interfilum cf. massjukiae",
"Interfilum terricola",
"Klebsormidium cf. flaccidum",
"Klebsormidium cf. subtile",
"Klebsormidium crenulatum",
"Lecania cyrtella",
"Lecanora hagenii",
"Lecanora persimilis",
"Leptolyngbya sp.",
"Lobochlamys sp.",
"Lophozia bicuspidata",
"Macrochloris sp.",
"Micarea misella",
"Microcoleus vaginatus",
"Myrmecia cf. irregularis",
"Nannochloris sp.",
"Nodosilinea cf. epilithica",
"Nostoc cf. commune",
"Nostoc cf. edaphicum",
"Nostoc cf. linckia",
"Nostoc sp.",
"Oxyrrhynchium praelongum",
"Parietochloris cf. alveolaris",
"Parmelia sulcata",
"Peltigera extenuata",
"Peltigera hymenia",
"Physcia tenella",
"Placynthiella uliginosa",
"Planophila sp.",
"Pleurozium schreberi",
"Pohlia nutans",
"Polytrichum juniperinum",
"Polytrichum piliferum",
"Pseudochlorella sp.",
"Pseudomuriella cf. aurantiaca",
"Pseudoscleropodium purum",
"Ptilidium ciliatum",
"Ptychostomum capillare",
"Ptychostomum compactum",
"Ptychostomum imbricatulum",
"Ptychostomum moravicum",
"Racomitrium canescens",
"Racomitrium elongatum",
"Scoliciosporum gallurae",
"Spongiochloris sp.",
"Stenomitos sp.",
"Stichococcus allas",
"Stichococcus cf. bacillaris",
"Syntrichia ruraliformis",
"Tetracystis cf. sarcinalis",
"Tetradesmus arenicola",
"Timaviella sp.",
"Tolypothrix cf. byssoidea",
"Watanabea cf. acidophila",
"Xanthoria parietina",
],
methods: [
"Light microscope, Olympus Ltd, BX53, with Nomarski DIC optics",
"Thin layer chromatography",
"Light microscope, Olympus Ltd, BX53, with Nomarski DIC optics",
"Thin layer chromatography",
"Light microscope",
"Light microscope, Olympus Ltd, BX53, with Nomarski DIC optics",
"Light microscope",
"Thin layer chromatography",
"Light microscope",
"Light microscope",
"Light microscope",
"Light microscope, Olympus Ltd, BX53, with Nomarski DIC optics",
"Light microscope, Olympus Ltd, BX53, with Nomarski DIC optics",
"Light microscope, Olympus Ltd, BX53, with Nomarski DIC optics",
"Light microscope, Olympus Ltd, BX53, with Nomarski DIC optics",
"Light microscope, Olympus Ltd, BX53, with Nomarski DIC optics",
"Thin layer chromatography",
"Thin layer chromatography",
"Thin layer chromatography",
"Thin layer chromatography",
"Thin layer chromatography",
"Thin layer chromatography",
"Thin layer chromatography",
"Thin layer chromatography",
"Thin layer chromatography",
"Thin layer chromatography",
"Thin layer chromatography",
"Thin layer chromatography",
"Thin layer chromatography",
"Thin layer chromatography",
"Thin layer chromatography",
"Thin layer chromatography",
"Thin layer chromatography",
"Thin layer chromatography",
"Thin layer chromatography",
"Thin layer chromatography",
"Thin layer chromatography",
"Thin layer chromatography",
"Light microscope, Olympus Ltd, BX53, with Nomarski DIC optics",
"Light microscope, Olympus Ltd, BX53, with Nomarski DIC optics",
"Light microscope, Olympus Ltd, BX53, with Nomarski DIC optics",
"Light microscope, Olympus Ltd, BX53, with Nomarski DIC optics",
"Light microscope",
"Light microscope, Olympus Ltd, BX53, with Nomarski DIC optics",
"Light microscope, Olympus Ltd, BX53, with Nomarski DIC optics",
"Light microscope, Olympus Ltd, BX53, with Nomarski DIC optics",
"Thin layer chromatography",
"Light microscope",
"Light microscope",
"Light microscope",
"Light microscope",
"Thin layer chromatography",
"Light microscope, Olympus Ltd, BX53, with Nomarski DIC optics",
"Light microscope, Olympus Ltd, BX53, with Nomarski DIC optics",
"Light microscope, Olympus Ltd, BX53, with Nomarski DIC optics",
"Light microscope, Olympus Ltd, BX53, with Nomarski DIC optics",
"Light microscope, Olympus Ltd, BX53, with Nomarski DIC optics",
"Thin layer chromatography",
"Thin layer chromatography",
"Thin layer chromatography",
"Light microscope, Olympus Ltd, BX53, with Nomarski DIC optics",
"Light microscope, Olympus Ltd, BX53, with Nomarski DIC optics",
"Light microscope",
"Light microscope, Olympus Ltd, BX53, with Nomarski DIC optics",
"Thin layer chromatography",
"Light microscope, Olympus Ltd, BX53, with Nomarski DIC optics",
"Light microscope, Olympus Ltd, BX53, with Nomarski DIC optics",
"Light microscope, Olympus Ltd, BX53, with Nomarski DIC optics",
"Light microscope, Olympus Ltd, BX53, with Nomarski DIC optics",
"Light microscope, Olympus Ltd, BX53, with Nomarski DIC optics",
"Light microscope, Olympus Ltd, BX53, with Nomarski DIC optics",
"Light microscope, Olympus Ltd, BX53, with Nomarski DIC optics",
"Light microscope, Olympus Ltd, BX53, with Nomarski DIC optics",
"Light microscope",
"Light microscope, Olympus Ltd, BX53, with Nomarski DIC optics",
"Thin layer chromatography",
"Thin layer chromatography",
"Thin layer chromatography",
"Thin layer chromatography",
"Thin layer chromatography",
"Light microscope, Olympus Ltd, BX53, with Nomarski DIC optics",
"Light microscope",
"Light microscope",
"Light microscope",
"Light microscope",
"Light microscope, Olympus Ltd, BX53, with Nomarski DIC optics",
"Light microscope, Olympus Ltd, BX53, with Nomarski DIC optics",
"Light microscope",
"Light microscope",
"Light microscope",
"Light microscope",
"Light microscope",
"Light microscope",
"Light microscope",
"Light microscope",
"Thin layer chromatography",
"Light microscope, Olympus Ltd, BX53, with Nomarski DIC optics",
"Light microscope, Olympus Ltd, BX53, with Nomarski DIC optics",
"Light microscope, Olympus Ltd, BX53, with Nomarski DIC optics",
"Light microscope, Olympus Ltd, BX53, with Nomarski DIC optics",
"Light microscope",
"Light microscope, Olympus Ltd, BX53, with Nomarski DIC optics",
"Light microscope, Olympus Ltd, BX53, with Nomarski DIC optics",
"Light microscope, Olympus Ltd, BX53, with Nomarski DIC optics",
"Light microscope, Olympus Ltd, BX53, with Nomarski DIC optics",
"Light microscope, Olympus Ltd, BX53, with Nomarski DIC optics",
"Thin layer chromatography",
],
}
Dataset
Comment: Encoding for species composition: presence = 1, absence = 0
Origins: /Wissenschaft/PANGAEA
Tags: Hamburg ? Cyanobakterien ? Flechte ? Lösungsmittel ? Geografische Koordinaten ? Bodenökologie ? Düne ? Mikroalgen ? Papier ? Software ? Chromatografie ? Studie ? Mikroorganismen ? Algen ? 16S rRNA ? Crusts ? sediment analysis ?
Region: Mecklenburg-Western Pomerania Verden (Aller), Lower Saxony, Germany Rügen, Mecklenburg Western Pomerania, Germany
Bounding boxes: 9.2492° .. 13.56943° x 52.938231° .. 54.605357°
License: cc-by/4.0
Language: Englisch/English
Issued: 2022-09-01
Modified: 2023-07-11
Time ranges: 2020-04-25 - 2020-05-05
Accessed 1 times.