Description: Laboratory experiments were conducted in the climate chambers at GEOMAR Helmholtz Centre for Ocean Research Kiel in the time between March and November 2018. Experiments were designed to study the effect of long-term (1 month) exposure to low salinity in osmoconforming invertebrates. The study organisms (Asterias rubens, Mytilus edulis, Littorina littorea, Diadumene lineata, Strongylocentrotus droebachiensis and Psammechinus milliaris) were collected in Kiel Fjord, Eckernförder Bight or the Kattegat from spring to autumn 2018. Organisms were acclimated to climate chamber conditions for 1 week (under habitat salinity, 14˚C, constant aeration) and then subjected to salinity acclimation for 1-2 weeks until the final salinity treatment level was reached. Then different salinity treatments were maintained for 4 weeks. Water physiochemistry (temperature, salinity, pH, nitrite, nitrate, phosphate) was recorded frequently. After the experiment, samples were taken from tissues to measure total osmolality (mosmol/kg) with an osmomat, and inorganic ions (mmol/kg or µmol/g wet mass). Anions were measured with a novel protocol via ion chromatography, cations were measured via flame photometry. Organic osmolytes were measured via 1H-NMR.
Global identifier:
Doi(
"10.1594/PANGAEA.959689",
)
DataMeasurements(
DataMeasurements {
domain: Unspecified,
station: None,
measured_variables: [
"Event label",
"Type of study",
"Species, unique identification",
"Species, unique identification (URI)",
"Species, unique identification (Semantic URI)",
"Date/time start, experiment",
"Date/time end, experiment",
"Temperature, water",
"Treatment: salinity",
"Tank number",
"Individual code",
"Medium",
"Bromide",
"Chloride",
"Fluoride",
"Nitrate",
"Phosphate",
"Sulfate",
"Calcium",
"Potassium",
"Sodium",
"Osmolality",
"Adenosine diphosphate, per wet mass",
"Adenosine monophosphate, per wet mass",
"Adenosine triphosphate, per wet mass",
"Acetate, per wet mass",
"Alanine",
"Anserine",
"Arginine",
"Asparagine",
"Aspartic acid",
"Betaine",
"Carnitine",
"Choline",
"Creatine",
"Creatinephosphate",
"Creatinine",
"Dimethylsulfone",
"Dimethylamine",
"Glutamic acid",
"Glutamine",
"Glycine",
"Histamine",
"Homarine 5",
"Homocysteine",
"Homoserine",
"Hydroxyacetone",
"Hypotaurine",
"Inosine",
"Isoleucine",
"L-Arginine",
"Lactate, per wet mass",
"Leucine",
"Lysine",
"Malonate",
"Methylamine",
"N-Methylhydantoin",
"Methylmalonate",
"O-Acetylcholine",
"O-Phosphocholine",
"Ornithine",
"Phenylalanine",
"Proline",
"Sarcosine",
"Serine",
"Succinate, per wet mass",
"Taurine",
"Threonine",
"Trimethylamine N-oxide",
"Tryptophan",
"Tyrosine",
"Metabolite, unassigned",
"Metabolite, unassigned",
"Metabolite, unassigned",
"Metabolite, unassigned",
"Valine",
"sn-Glycero-3-phosphocholine",
"beta-Alanine",
"pi-Methylhistidine",
"tau-Methylhistidine",
],
methods: [
"Visual identification",
"Visual identification",
"Visual identification",
"Calculated average/mean values",
"Ion chromatography",
"Ion chromatography",
"Ion chromatography",
"Ion chromatography",
"Ion chromatography",
"Ion chromatography",
"Flame photometry",
"Flame photometry",
"Flame photometry",
"Freeze-depression osmometer, Gonotec, Osmomat 030",
"1H NMR spectroscopy",
"1H NMR spectroscopy",
"1H NMR spectroscopy",
"1H NMR spectroscopy",
"1H NMR spectroscopy",
"1H NMR spectroscopy",
"1H NMR spectroscopy",
"1H NMR spectroscopy",
"1H NMR spectroscopy",
"1H NMR spectroscopy",
"1H NMR spectroscopy",
"1H NMR spectroscopy",
"1H NMR spectroscopy",
"1H NMR spectroscopy",
"1H NMR spectroscopy",
"1H NMR spectroscopy",
"1H NMR spectroscopy",
"1H NMR spectroscopy",
"1H NMR spectroscopy",
"1H NMR spectroscopy",
"1H NMR spectroscopy",
"1H NMR spectroscopy",
"1H NMR spectroscopy",
"1H NMR spectroscopy",
"1H NMR spectroscopy",
"1H NMR spectroscopy",
"1H NMR spectroscopy",
"1H NMR spectroscopy",
"1H NMR spectroscopy",
"1H NMR spectroscopy",
"1H NMR spectroscopy",
"1H NMR spectroscopy",
"1H NMR spectroscopy",
"1H NMR spectroscopy",
"1H NMR spectroscopy",
"1H NMR spectroscopy",
"1H NMR spectroscopy",
"1H NMR spectroscopy",
"1H NMR spectroscopy",
"1H NMR spectroscopy",
"1H NMR spectroscopy",
"1H NMR spectroscopy",
"1H NMR spectroscopy",
"1H NMR spectroscopy",
"1H NMR spectroscopy",
"1H NMR spectroscopy",
"1H NMR spectroscopy",
"1H NMR spectroscopy",
"1H NMR spectroscopy",
"1H NMR spectroscopy",
"1H NMR spectroscopy",
"1H NMR spectroscopy",
"1H NMR spectroscopy",
"1H NMR spectroscopy",
"1H NMR spectroscopy",
"1H NMR spectroscopy",
"1H NMR spectroscopy",
"1H NMR spectroscopy",
],
},
)
Origins: /Wissenschaft/PANGAEA
Tags: Nitrit ? Nitrat ? Wassertemperatur ? Habitat ? Wirbellose ? Salzgehalt ? Studie ? Laborversuch ? Meeresgewässer ? Klimaforschung ? Klimawandel ? Wasseraufbereitung ? cellular volume regulation ? osmoconformer ? osmolytes ? osmoregulation ? salinity tolerance ?
Region: Kiel Bay Baltic Sea, Kattegat Baltic Sea, Eckernförde Bay
Bounding boxes: 10.135° .. 11.35111111° x 54.31861111° .. 56.4025°
License: cc-by/4.0
Language: Englisch/English
Issued: 2023-06-20
Modified: 2023-06-20
Time ranges: 2023-06-20 - 2023-06-20
Accessed 1 times.