Description: To investigate the effect of temperature on a North Sea spring bloom community, we performed an incubation experiment in the mesocosm facility of the Institute for Chemistry and Biology of the Marine Environment (ICBM) in Wilhelmshaven. The plankton community was sampled from the long-term ecological research station Helgoland Roads (https://deims.org/1e96ef9b-0915-4661-849f-b3a72f5aa9b1) on the 6ᵗʰ of March, 2022. Collection of the surface community was conducted from the RV Heincke with a pipe covered with a 200 µm net that was attached to a diaphragm pump. The month-long incubation was started on the 7ᵗʰ of March in twelve indoor mesocosms, the Planktotrons (Gall et al., 2017). We chose three temperatures along the ascending part of the thermal performance curve (TPC) of the in situ community: the minimum temperature for positive growth (6°C, also the field temperature), the middle between the minimum and the optimum temperature (12 °C), and the optimum temperature for growth (18 °C). Ramping up the temperatures was conducted by 1 °C per day until the treatment temperatures were reached, resulting in a ramp phase (first twelve days) and a constant temperature phase. This dataset comprises all data collected within the experiment. Temperature, oxygen, pH, salinity, and in vivo fluorescence were measured daily at 10 am. Samples for dissolved nutrients (nitrate, nitrite, phosphate, silicate), chlorophyll a, DNA, particulate nutrients (biogenic silica, particulate organic carbon/nitrogen/phosphorus), as well as flow cytometric counts of bacteria (stained) and the unstained community were sampled every third day at the same time. The mesocosm water was generally filtered over a 200 µm mesh before sampling to exclude mesozooplankton. However, due to the appearance of large Phaeocystis colonies, additional samples without pre-filtration were taken for particulate organic carbon, nitrogen, phosphorus, and chlorophyll a starting on incubation day 15. PAR, total nitrogen and phosphorus as well as total alkalinity were measured at the start, in the middle, and at the end of the incubation. Samples for Mesozooplankton enumeration were taken and plankton species identified at the end of the experiment. All analysis scripts can be found on github (https://github.com/AntoniaAhme/TopTrons22MesocosmIncubation). The sequence data are available at the European Nucleotide Archive (ENA).
Global identifier:
Doi(
"10.1594/PANGAEA.961155",
)
DataMeasurements(
DataMeasurements {
domain: Unspecified,
station: None,
measured_variables: [
"Alkalinity, total",
"Bacteria",
"Bicarbonate ion",
"Biogenic silica",
"Carbon dioxide, dissolved",
"Carbon dioxide, partial pressure",
"Carbon trioxide",
"Carbon, inorganic, dissolved",
"Carbon, organic, particulate",
"Chlorophyll a",
"Cryptophytes",
"Cyanobacteria",
"Date/time end, experiment",
"Date/time start, experiment",
"Event label",
"Fluorescence, in vivo",
"Incubation duration",
"Mesocosm label",
"Mesozooplankton",
"Method comment",
"Nitrate and Nitrite",
"Nitrogen, organic, particulate",
"Nitrogen, total dissolved",
"Oxygen",
"Phosphate",
"Phosphorus, organic, particulate",
"Phosphorus, total dissolved",
"Phytoplankton, total",
"Plankton",
"Radiation, photosynthetically active",
"Replicate",
"Salinity",
"Sample code/label",
"Silicate",
"Species",
"Temperature, water",
"Treatment: temperature",
"Treatment: temperature description",
"Type of study",
"pH, NBS scale",
],
methods: [
"Carbon and hydrogen and nitrogen (CHN) analyzer, Thermo Scientific, FlashEA 1112",
"Cell Sorter, BD Biosciences, BD Influx",
"Conductivity and oxygen sensor, WTW, ConOx-1.5; measured with Handheld meter, WTW, Multi 3630 IDS",
"Conductivity probe, WTW, TetraCon 925/C; measured with Handheld meter, WTW, Multi 3630 IDS",
"Elemental analyzer, EuroVector, EA 3000",
"Flow cytometer, BD Biosciences, BD Accuri C6",
"Handheld Fluorometer, Turner Designs, AquaFluor",
"Measured according to Grasshoeff et al. (2009)",
"Measured according to Wetzel and Likens (2003)",
"Microplate reader, BioTek, Synergy H1",
"Oxygen sensor, PreSens Precision Sensing GmbH, OXYBase WR-RS485-L5",
"Pressure resistant ORP electrode, WTW, SensoLyt ORP 900-P; measured with Handheld meter, WTW, Multi 3630 IDS",
"Segmented Flow Analyzer, SEAL Analytical, SEAL AA500",
"Stereo microscope, Leica Microsystems, S8 APO",
"Temperature probe, TC Mess- und Regeltechnik GmbH, PT100; measured with Temperature controller, Temperature Control GmbH, custom-built",
"Titration analyzer, Schott Instruments, TitroLine alpha plus",
"calculated from carbonate chemistry using the CO2Sys Excel sheet (Pierrot, Lewis & Wallace, 2006)",
],
},
)
Comment: For more information about the research site Helgoland Roads visit https://deims.org/1e96ef9b-0915-4661-849f-b3a72f5aa9b1
Origins: /Wissenschaft/Helmholtz-Gemeinschaft /Wissenschaft/PANGAEA
Tags: Nitrit ? Siliziumdioxid ? Nitrat ? Helgoland ? Cyanobakterien ? Nährstoff ? Phosphor ? Phytoplankton ? Wassertemperatur ? Bakterien ? Gesamtkohlenstoff ? Gesamtstickstoff ? Kohlendioxid ? Marines Ökosystem ? Plankton ? Strahlung ? Salzgehalt ? Daten ? Studie ? Wasserprobe ? Wasser ? Freilandversuch ? Rohrleitung ? Wasseraufbereitung ? Nordsee ? community composition ? mesocosm experiment ? mesozooplankton ? plankton community ? spring bloom ? stoichiometry ? temperature stress ? Planktotron ? thermal performance curve ? incubation experiment ?
Region: North Sea
Bounding boxes: 7.9° .. 7.9° x 54.183333° .. 54.183333°
License: cc-by/4.0
Language: Englisch/English
Issued: 2023-08-25
Modified: 2024-02-12
Time ranges: 2022-03-06 - 2022-03-06
Accessed 1 times.