Description: Surface sediment were extracted 4 times by ultrasonication with dichloromethane: methanol (9:1, v/v) for 15 min for FAs and alkanes. For quantification of FAs and alkanes, known amounts of 19-methylarachidic acid and squalane were added as internal standards prior to extraction. Supernatants from each extraction were obtained by centrifugation and combined. The total lipid extracts were concentrated and evaporated under a nitrogen stream. The total lipid extracts were saponified for 2 h at 80 °C with 1 mL of KOH (0.1 M) in methanol: H2O (9:1, v/v). After saponification, the neutral fractions were liquid-liquid extracted with n-hexane and alkanes were eluted from the neutral fractions by silica gel column chromatography with n-hexane. The remaining KOH solution was acidified to pH 1, from which FA were liquid-liquid extracted into dichloromethane. The extracted and dried FAs were converted to methyl ester derivatives (FAMEs) in methanol: HCl (95:5, v/v) at 60 °C for 12 h. After methylation, the FAME fraction was further purified by silica gel column chromatography using dichloromethane: hexane (2:1, v/v) to remove residual polar compounds. FAMEs and alkanes were analyzed on a 7890A gas chromatograph (GC) equipped with a DB-5MS fused silica capillary column (60 m, 250 µm, 0.25 µm) and a flame ionization detector (FID). Peak areas were determined by integrating the respective peaks and concentrations were calculated against the internal standards. FAME contents were subsequently corrected for the derivative methyl carbon to determine FA contents. FAs and alkanes were normalized to OC content.
Global identifier:
Doi(
"10.1594/PANGAEA.971522",
)
Measurements {
domain: Unspecified,
station: None,
measured_variables: [
"Event label",
"LATITUDE",
"LONGITUDE",
"DEPTH, sediment/rock",
"Depth, top/min",
"Depth, bottom/max",
"n-Alkane C14, per unit mass total organic carbon",
"n-Alkane C15, per unit mass total organic carbon",
"n-Alkane C16, per unit mass total organic carbon",
"n-Alkane C17, per unit mass total organic carbon",
"Pristane, per unit mass total organic carbon",
"n-Alkane C18, per unit mass total organic carbon",
"Phytane, per unit mass total organic carbon",
"n-Alkane C19, per unit mass total organic carbon",
"n-Alkane C20, per unit mass total organic carbon",
"n-Alkane C21, per unit mass total organic carbon",
"n-Alkane C22, per unit mass total organic carbon",
"n-Alkane C23, per unit mass total organic carbon",
"n-Alkane C24, per unit mass total organic carbon",
"n-Alkane C25, per unit mass total organic carbon",
"n-Alkane C26, per unit mass total organic carbon",
"n-Alkane C27, per unit mass total organic carbon",
"n-Alkane C28, per unit mass total organic carbon",
"n-Alkane C29, per unit mass total organic carbon",
"n-Alkane C30, per unit mass total organic carbon",
"n-Alkane C31, per unit mass total organic carbon",
"n-Alkane C32, per unit mass total organic carbon",
"n-Alkane C33, per unit mass total organic carbon",
"n-Alkane C34, per unit mass total organic carbon",
"n-Alkane C35, per unit mass total organic carbon",
"n-Alkane C36, per unit mass total organic carbon",
"n-Alkanes, sum, per unit mass total organic carbon",
"n-Alkanes C27+C29+C31+C35 per unit mass total organic carbon",
"n-Alkanes C26+C28+C30+C32 per unit mass total organic carbon",
"n-Alkane C27+C29+C31+C35 loading",
"n-Alkane C26+C28+C30+C32 loading",
],
methods: [
"Gas chromatograph, Agilent, 7890A; with flame ionization detection (FID)",
"Gas chromatograph, Agilent, 7890A; with flame ionization detection (FID)",
"Gas chromatograph, Agilent, 7890A; with flame ionization detection (FID)",
"Gas chromatograph, Agilent, 7890A; with flame ionization detection (FID)",
"Gas chromatograph, Agilent, 7890A; with flame ionization detection (FID)",
"Gas chromatograph, Agilent, 7890A; with flame ionization detection (FID)",
"Gas chromatograph, Agilent, 7890A; with flame ionization detection (FID)",
"Gas chromatograph, Agilent, 7890A; with flame ionization detection (FID)",
"Gas chromatograph, Agilent, 7890A; with flame ionization detection (FID)",
"Gas chromatograph, Agilent, 7890A; with flame ionization detection (FID)",
"Gas chromatograph, Agilent, 7890A; with flame ionization detection (FID)",
"Gas chromatograph, Agilent, 7890A; with flame ionization detection (FID)",
"Gas chromatograph, Agilent, 7890A; with flame ionization detection (FID)",
"Gas chromatograph, Agilent, 7890A; with flame ionization detection (FID)",
"Gas chromatograph, Agilent, 7890A; with flame ionization detection (FID)",
"Gas chromatograph, Agilent, 7890A; with flame ionization detection (FID)",
"Gas chromatograph, Agilent, 7890A; with flame ionization detection (FID)",
"Gas chromatograph, Agilent, 7890A; with flame ionization detection (FID)",
"Gas chromatograph, Agilent, 7890A; with flame ionization detection (FID)",
"Gas chromatograph, Agilent, 7890A; with flame ionization detection (FID)",
"Gas chromatograph, Agilent, 7890A; with flame ionization detection (FID)",
"Gas chromatograph, Agilent, 7890A; with flame ionization detection (FID)",
"Gas chromatograph, Agilent, 7890A; with flame ionization detection (FID)",
"Gas chromatograph, Agilent, 7890A; with flame ionization detection (FID)",
"Gas chromatograph, Agilent, 7890A; with flame ionization detection (FID)",
],
}
Dataset
Origins: /Wissenschaft/PANGAEA
Tags: Gesamter organischer Kohlenstoff ? Alkan ? Methanol ? Siliziumdioxid ? Methylester ? Sensor ? Stickstoff ? Geografische Koordinaten ? Chromatografie ? Sediment ? Rückstand ? Nordsee ? Carbon Burial ? Helgoland mud area ?
Region: North Sea
Bounding boxes: 7.919804° .. 8.7072° x 53.8983° .. 54.23°
License: cc-by/4.0
Language: Englisch/English
Issued: 2025-02-19
Modified: 2025-03-31
Time ranges: 2022-03-17 - 2022-03-30
Accessed 1 times.