Description: Surface sediment were extracted 4 times by ultrasonication with dichloromethane: methanol (9:1, v/v) for 15 min for FAs and alkanes. For quantification of FAs and alkanes, known amounts of 19-methylarachidic acid and squalane were added as internal standards prior to extraction. Supernatants from each extraction were obtained by centrifugation and combined. The total lipid extracts were concentrated and evaporated under a nitrogen stream. The total lipid extracts were saponified for 2 h at 80 °C with 1 mL of KOH (0.1 M) in methanol: H2O (9:1, v/v). After saponification, the neutral fractions were liquid-liquid extracted with n-hexane and alkanes were eluted from the neutral fractions by silica gel column chromatography with n-hexane. The remaining KOH solution was acidified to pH 1, from which FA were liquid-liquid extracted into dichloromethane. The extracted and dried FAs were converted to methyl ester derivatives (FAMEs) in methanol: HCl (95:5, v/v) at 60 °C for 12 h. After methylation, the FAME fraction was further purified by silica gel column chromatography using dichloromethane: hexane (2:1, v/v) to remove residual polar compounds. FAMEs and alkanes were analyzed on a 7890A gas chromatograph (GC) equipped with a DB-5MS fused silica capillary column (60 m, 250 µm, 0.25 µm) and a flame ionization detector (FID). Peak areas were determined by integrating the respective peaks and concentrations were calculated against the internal standards. FAME contents were subsequently corrected for the derivative methyl carbon to determine FA contents. FAs and alkanes were normalized to OC content.
Global identifier:
Doi(
"10.1594/PANGAEA.971523",
)
Measurements {
domain: Unspecified,
station: None,
measured_variables: [
"Event label",
"LATITUDE",
"LONGITUDE",
"DEPTH, sediment/rock",
"Depth, top/min",
"Depth, bottom/max",
"Tetradecanoic acid, per unit mass total organic carbon",
"13-methyl-Tetradecanoic acid, per unit mass total organic carbon",
"12-methyl-Tetradecanoic acid, per unit mass total organic carbon",
"Pentadecanoic acid, per unit mass total organic carbon",
"Hexadecanoic acid, per unit mass total organic carbon",
"15-methyl-Hexadecanoic acid, per unit mass total organic carbon",
"14-methyl-Hexadecanoic acid, per unit mass total organic carbon",
"Heptadecanoic acid, per unit mass total organic carbon",
"Octadecanoic acid, per unit mass total organic carbon",
"Octadecanoic acid, per unit mass total organic carbon",
"Icosanoic acid, per unit mass total organic carbon",
"Heneicosanoic acid, per unit mass total organic carbon",
"Docosanoic acid, per unit mass total organic carbon",
"Tricosanoic acid, per unit mass total organic carbon",
"Tetracosanoic acid, per unit mass total organic carbon",
"Pentacosanoic acid, per unit mass total organic carbon",
"Hexacosanoic acid, per unit mass total organic carbon",
"Heptacosanoic acid, per unit mass total organic carbon",
"Octacosanoic acid, per unit mass total organic carbon",
"Nonacosanoic acid, per unit mass total organic carbon",
"Triacontanoic acid, per unit mass total organic carbon",
"Hentriacontanoic acid, per unit mass total organic carbon",
"Dotriacontanoic acid, per unit mass total organic carbon",
"Fatty acids, per unit mass total organic carbon",
"Fatty acids, per unit mass total organic carbon",
"Fatty acids, per unit mass total organic carbon",
"Carbon Preference Index, fatty acids",
"Fatty acids, per unit mass total organic carbon",
"Fatty acids, per unit mass marine organic carbon",
"Fatty acids, per unit mass terrestrial organic carbon",
"Fatty acids, loading",
"Fatty acids, loading",
],
methods: [
"Gas chromatograph, Agilent, 7890A; with flame ionization detection (FID)",
"Gas chromatograph, Agilent, 7890A; with flame ionization detection (FID)",
"Gas chromatograph, Agilent, 7890A; with flame ionization detection (FID)",
"Gas chromatograph, Agilent, 7890A; with flame ionization detection (FID)",
"Gas chromatograph, Agilent, 7890A; with flame ionization detection (FID)",
"Gas chromatograph, Agilent, 7890A; with flame ionization detection (FID)",
"Gas chromatograph, Agilent, 7890A; with flame ionization detection (FID)",
"Gas chromatograph, Agilent, 7890A; with flame ionization detection (FID)",
"Gas chromatograph, Agilent, 7890A; with flame ionization detection (FID)",
"Gas chromatograph, Agilent, 7890A; with flame ionization detection (FID)",
"Gas chromatograph, Agilent, 7890A; with flame ionization detection (FID)",
"Gas chromatograph, Agilent, 7890A; with flame ionization detection (FID)",
"Gas chromatograph, Agilent, 7890A; with flame ionization detection (FID)",
"Gas chromatograph, Agilent, 7890A; with flame ionization detection (FID)",
"Gas chromatograph, Agilent, 7890A; with flame ionization detection (FID)",
"Gas chromatograph, Agilent, 7890A; with flame ionization detection (FID)",
"Gas chromatograph, Agilent, 7890A; with flame ionization detection (FID)",
"Gas chromatograph, Agilent, 7890A; with flame ionization detection (FID)",
"Gas chromatograph, Agilent, 7890A; with flame ionization detection (FID)",
"Gas chromatograph, Agilent, 7890A; with flame ionization detection (FID)",
"Gas chromatograph, Agilent, 7890A; with flame ionization detection (FID)",
"Gas chromatograph, Agilent, 7890A; with flame ionization detection (FID)",
"Gas chromatograph, Agilent, 7890A; with flame ionization detection (FID)",
],
}
Dataset
Origins: /Wissenschaft/PANGAEA
Tags: Gesamter organischer Kohlenstoff ? Alkan ? Methanol ? Siliziumdioxid ? Methylester ? Sensor ? Stickstoff ? Geografische Koordinaten ? Chromatografie ? Sediment ? Rückstand ? Nordsee ? Carbon Burial ? Helgoland mud area ? fatty acids ?
Region: North Sea
Bounding boxes: 7.919804° .. 8.7072° x 53.8983° .. 54.23°
License: cc-by/4.0
Language: Englisch/English
Issued: 2025-02-19
Modified: 2025-03-31
Time ranges: 2022-03-17 - 2022-03-30
Accessed 1 times.