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Total carbohydrates quantified in sediment cores from coastal vegetated ecosystems

Description: 50-cm deep sediment cores were taken in saltmarsh, seagrass, mangroves and unvegetated areas around the German Bight, Malaysia and Columbia in 2022 and 2023. Up to 3 points per ecosystem were sampled along a transect, in total 93 cores were analysed. Carbohydrates were sequentially extracted using MilliQ-water and 0.3 M EDTA for later analyses. The total carbohydrate content was assessed using the phenol-sulfuric acid assay (Dubois et al., 1956). Briefly, 100 µL of resuspended samples or extracts were mixed with 100 µL of 5% phenol solution, followed by the addition of 500 µL of concentrated sulfuric acid. The reaction mixture was incubated at room temperature for 10 minutes, then further incubated at 30°C for 20 minutes. Absorbance at 490 nm was measured using a Spectramax Id3 plate reader (Molecular Devices) and quantified against a glucose standard curve.

Global identifier:

Doi(
    "10.1594/PANGAEA.979910",
)

Types:
DataMeasurements(
    DataMeasurements {
        domain: Unspecified,
        station: None,
        measured_variables: [
            "Carbohydrates, total, per unit sediment dry mass",
            "Comment",
            "Country",
            "DATE/TIME",
            "DEPTH, sediment/rock",
            "Depth, sediment/rock, bottom/maximum",
            "Depth, sediment/rock, top/minimum",
            "Ecosystem",
            "Event label",
            "Identification",
            "LATITUDE",
            "LONGITUDE",
            "Layer description",
            "Layer number",
            "Location",
            "Ocean and sea region",
            "Sample code/label",
        ],
        methods: [
            "Phenol-sulfuric acid assay according to Dubois et al. (1956)",
        ],
    },
)

Tags: Phenol ? Kohlenhydrat ? Schwefelsäure ? Geografische Koordinaten ? Temperatur ? Sediment ? Meeresgewässer ? Deutsche Bucht ? Carbon Dioxide Removal ? Ökosystem ? Glycans ? coastal vegetated ecosystems ? marine carbon sequestration ?

Region: Colombia

Bounding boxes: -75.682996° .. 103.439555° x 4.658409° .. 54.692271°

License: cc-by/4.0

Language: Englisch/English

Organisations

Persons

Issued: 2025-04-28

Modified: 2026-01-14

Time ranges: 2021-11-08 - 2022-10-11

Resources

Status

Quality score

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