Description: 50-cm deep sediment cores were taken in saltmarsh, seagrass, mangroves and unvegetated areas around the German Bight, Malaysia and Columbia in 2022 and 2023. Up to 3 points per ecosystem were sampled along a transect, in total 93 cores were analysed. Carbohydrates were sequentially extracted using MilliQ-water and 0.3 M EDTA for later analyses. Polysaccharides were screened using microarray analysis following the method described by Vidal-Melgosa et al. (2022). Briefly, sediment extracts from MilliQ-water and EDTA were combined in equal volumes, and 30 µL of the mixture was transferred into wells of 384-microwell plates. Two consecutive two-fold dilutions were performed using a printing buffer (55.2% glycerol, 44% water, 0.8% Triton X-100). The plates were then centrifuged at 3,500 × g for 10 minutes at 15 °C. Each microarray was individually probed with a monoclonal antibody (mAb), and binding was detected using a secondary antibody conjugated to alkaline phosphatase. In the presence of its substrate, this reaction produced a colorimetric signal. Developed arrays were scanned at 2400 dots per inch, and binding signal intensity was quantified using Array-Pro Analyzer 6.3 software (Media Cybernetics).
Global identifier:
Doi(
"10.1594/PANGAEA.979911",
)
DataMeasurements(
DataMeasurements {
domain: Unspecified,
station: None,
measured_variables: [
"Alginate mannuronate-guluronate, relative BAM7 antibody signal",
"Arabinogalactan protein glycan, relative JIM13 antibody signal",
"Arabinogalactan protein glycan, relative JIM8 antibody signal",
"Arabinogalactan protein glycan, relative MAC207 antibody signal",
"Country",
"DATE/TIME",
"DEPTH, sediment/rock",
"Depth comment",
"Depth, sediment/rock, bottom/maximum",
"Depth, sediment/rock, top/minimum",
"Ecosystem",
"Event label",
"Fucan, sulfated epitope, relative BAM2 antibody signal",
"Fucan, sulfated epitope, relative BAM4 antibody signal",
"Grass xylan, relative LM27 antibody signal",
"Homogalacturonan, methyl-esterified, relative JIM7 antibody signal",
"Homogalacturonan, partially methyl-esterified/de-esterified, relative JIM5 antibody signal",
"LATITUDE",
"LONGITUDE",
"Location",
"Rhamnogalacturonan I backbone, relative INRA-RU1 antibody signal",
"Sample code/label",
"alpha-1,5-L-arabinan, relative LM6 antibody signal",
"alpha-1,5-L-arabinan, relative LM6-M antibody signal",
"beta-1,3-D-glucan, relative BS-400-2 antibody signal",
"beta-1,4-D-galactan, relative LM5 antibody signal",
],
methods: [
"Microarray analysis according to Vidal-Melgosa et al. (2022)",
],
},
)
Comment: The samples were analysed on 2024-10-09 including the standards for the microarray analysis.
Tags: Süßgräser ? Kohlenhydrat ? Geografische Koordinaten ? Software ? Sediment ? Wasser ? Deutsche Bucht ? Carbon Dioxide Removal ? Ökosystem ? Glycans ? coastal vegetated ecosystems ? marine carbon sequestration ?
Region: Colombia
Bounding boxes: -75.682996° .. 11.02179° x 10.136577° .. 54.692271°
License: cc-by/4.0
Language: Englisch/English
Issued: 2025-04-28
Modified: 2026-01-14
Time ranges: 2021-11-08 - 2022-08-30
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