API src

Polysaccharide fucoidan BAM1 and arabinogalactan-protein glycan JIM13 antibody binding in sediment cores from coastal vegetated ecosystems

Description: 50-cm deep sediment cores were taken in saltmarsh, seagrass, mangroves and unvegetated areas around the German Bight, Malaysia and Columbia in 2022 and 2023. Up to 3 points per ecosystem were sampled along a transect, in total 93 cores were analysed. Carbohydrates were sequentially extracted using MilliQ-water and 0.3 M EDTA for later analyses. For more specific analysis enzyme-linked immunosorbent assay (ELISA) was used for detection of fucoidan and arabinogalactan-protein glycan as described in the following studies (Vidal-Melgosa et al., 2021 and Cornuault et al., 2014). In short, 100 µL of sediment extracts were added to a pre-coated 96-well plate and incubated overnight at 4°C. The signal was developed using primary antibodies, BAM1 (fucoidan) and JIM13 (arabinogalactan-protein glycan), diluted 1:10 in skim milk PBS solution, followed by anti-rat antibody at a 1:1000 dilution in the same solution. Absorbance was measured at 450 nm using a Spectramax Id3 plate reader (Molecular Devices).

Global identifier: 

Doi(
    "10.1594/PANGAEA.979912",
)

Types: 
DataMeasurements(
    DataMeasurements {
        domain: Unspecified,
        station: None,
        measured_variables: [
            "Arabinogalactan protein glycan, relative JIM13 antibody signal",
            "Comment",
            "Country",
            "DATE/TIME",
            "DEPTH, sediment/rock",
            "Depth comment",
            "Depth, sediment/rock, bottom/maximum",
            "Depth, sediment/rock, top/minimum",
            "Ecosystem",
            "Event label",
            "Identification",
            "LATITUDE",
            "LONGITUDE",
            "Layer description",
            "Layer number",
            "Location",
            "Polysaccharides, relative BAM1 antibody signal",
            "Sample code/label",
        ],
        methods: [
            "Enzyme-linked immunosorbent assay (ELISA) according to Cornuault et al. (2014)",
        ],
    },
)

Comment: The samples were analysed on 2024-08-07 including the processing blanks for ELISA analysis.

Tags: Kohlenhydrat ? Geografische Koordinaten ? Studie ? Sediment ? Deutsche Bucht ? Carbon Dioxide Removal ? Ökosystem ? Glycans ? coastal vegetated ecosystems ? marine carbon sequestration ?

Region: Colombia

Bounding boxes: -75.682996° .. 103.439555° x 4.660548° .. 54.692271°

License: cc-by/4.0

Language: Englisch/English

Organisations

Persons

Issued: 2025-04-28

Modified: 2026-01-14

Time ranges: 2021-11-08 - 2022-10-11

Resources

Status

Quality score

Accessed 1 times.