API src

Physiological data of Pacific oyster Crassostrea (Magallana) gigas after exposure to intermittent hypoxia and the combination with (fluctuating) elevated temperature

Description: Organisms in intertidal zones experience fluctuations in environmental stressors such as hypoxia and temperature. These stressors and their fluctuations often appear in combination. Combination of stressors can have different effects compared to single stressors. In this study, we investigate the physiological effects of intermittent hypoxia in combination with different temperature regimes on the Pacific Oyster Crassostrea (Magallana) gigas. The oysters were exposed to hypoxic cycles (12h hypoxia by emersion/12h submersion) at normal (15°C), elevated (30°C) or fluctuating (15°C submersion/30°C emersion) temperature for 10 days. After the last submersion phase, the gills and digestive gland were sampled. We measured markers for bioenergetics and redox-balance in the gills and digestive gland using colorimetric methods as well as a set of metabolites (predominantly amino acids, osmolytes, anaerobic end products and energetic metabolites) in the gills using LC-MS/MS. Oysters kept submerged for up to 10 days were used as controls.

Global identifier: 

Doi(
    "10.1594/PANGAEA.980734",
)

Types: 
Measurements {
    domain: Unspecified,
    station: None,
    measured_variables: [
        "Experiment",
        "Type of study",
        "Laboratory",
        "Species, unique identification",
        "Origin",
        "Date/time end, experiment",
        "Treatment: temperature",
        "Treatment: temperature",
        "Treatment: air exposure",
        "Treatment: salinity",
        "Mass",
        "Length",
        "Width",
        "Sample ID",
        "Specimen identification",
        "Tank number",
        "Experimental treatment",
        "Tissue type",
        "Sampling date/time, experiment",
        "Experiment duration",
        "Carbohydrates, per fresh mass",
        "Electron transport system activity of oyxgen",
        "Proteins per fresh mass",
        "Lipid peroxidation, per fresh mass",
        "Protein carbonyl, per protein mass",
        "Antioxidant capacity, in Trolox Equivalents, per fresh mass",
        "Cystine, per fresh mass",
        "Asparagine, per fresh mass",
        "Aspartate, per fresh mass",
        "Serine, per fresh mass",
        "Alanine, per fresh mass",
        "Glycine, per fresh mass",
        "Glutamine, per fresh mass",
        "Threonine, per fresh mass",
        "Methionine sulfoxide, per fresh mass",
        "Glutamate, per fresh mass",
        "Proline, per fresh mass",
        "Lysine, per fresh mass",
        "Histidine, per fresh mass",
        "Adenosine monophosphate, per fresh mass",
        "Arginine, per fresh mass",
        "Valine, per fresh mass",
        "Methionine, per fresh mass",
        "Tyrosine, per fresh mass",
        "Isoleucine, per fresh mass",
        "Leucine, per fresh mass",
        "Phenylalanine, per fresh mass",
        "Tryptophan, per fresh mass",
        "Malate, per fresh mass",
        "Citrate, per fresh mass",
        "Succinate, per fresh mass",
        "Taurine, per fresh mass",
        "Lactate, per fresh mass",
        "Aconitate, per fresh mass",
        "gamma-Aminobutyric acid, per fresh mass",
        "Carnitine, per fresh mass",
        "Citrulline, per fresh mass",
        "Ornithine, per fresh mass",
        "Glutathione, per fresh mass",
        "Hydroxyproline, per fresh mass",
    ],
    methods: [
        "Caliper",
        "Caliper",
        "Multi-user microplate reader, Molecular devices, SpectraMax iD3",
        "Multi-user microplate reader, Molecular devices, SpectraMax iD3",
        "Multi-user microplate reader, Molecular devices, SpectraMax iD3",
        "Multi-user microplate reader, Molecular devices, SpectraMax iD3",
        "Multi-user microplate reader, Molecular devices, SpectraMax iD3",
        "Multi-user microplate reader, Molecular devices, SpectraMax iD3",
        "High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050",
        "High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050",
        "High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050",
        "High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050",
        "High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050",
        "High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050",
        "High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050",
        "High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050",
        "High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050",
        "High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050",
        "High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050",
        "High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050",
        "High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050",
        "High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050",
        "High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050",
        "High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050",
        "High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050",
        "High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050",
        "High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050",
        "High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050",
        "High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050",
        "High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050",
        "High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050",
        "High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050",
        "High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050",
        "High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050",
        "High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050",
        "High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050",
        "High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050",
        "High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050",
        "High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050",
        "High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050",
        "High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050",
        "High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050",
    ],
}
Dataset

Origins: /Wissenschaft/PANGAEA

Tags: Auster ? Ökophysiologie ? Physiologische Wirkung ? Kohlenhydrat ? Sauerstoff ? Gezeitenzone ? Wattenmeer ? Invasive Arten ? Lufttemperatur ? Temperatur ? Salzgehalt ? Daten ? Studie ? Laborversuch ? Energiereserven ? Stoffwechsel ? Verkehrssystem ? Magallana gigas ? bioenergetics ? crassotrea gigas ? fluctuation ? hypoxia ? intertidal ? metabolome ? metabolomics ? oxidaitive stress ? oxidative damage ? tidal cycle ?

Region: Königshafen

Bounding boxes: 8.43697° .. 8.43697° x 55.02906° .. 55.02906° 8.41589° .. 8.41589° x 55.04137° .. 55.04137°

License: cc-by/4.0

Language: Englisch/English

Organisations

Persons

Issued: 2025-04-09

Modified: 2025-04-10

Time ranges: 2020-06-17 - 2020-06-17

Resources

Status

Quality score

Accessed 1 times.