Chlorophyll a, extracellular polymeric substance concentration and 16S rRNA gene copy numbers in sediments in response to a marine heatwave

Description: This dataset originates from a laboratory mesocosm experiment investigating the effects of simulated marine heatwaves (MHWs) on benthic microbial communities from sublittoral sediments of Spiekeroog (Wadden Sea, Germany). Surface sediments were sieved (1 mm) and incubated under controlled light and temperature conditions before exposure to three temperature treatments (19 °C control, 23 °C, and 25 °C). Following a gradual warming phase, MHW conditions were maintained for six days, followed by a recovery phase at ambient temperature. Sediment samples were collected repeatedly throughout the experiment. Surface sediments (upper 2 cm) were collected using syringe cores. Pooled samples were analyzed for chlorophyll a as a proxy for microphytobenthos biomass using ethanol extraction and spectrophotometric pigment analysis. Extracellular polymeric substances (EPS) were quantified using EDTA extraction followed by phenol–sulfuric acid carbohydrate analysis. DNA was extracted from sediment subsamples using a Qiagen PowerSoil kit. Prokaryotic abundance was estimated by quantitative PCR targeting the 16S rRNA gene (primers 519F/907R), using an Escherichia coli 16S rRNA gene standard curve. The dataset includes chlorophyll a concentrations (µg g⁻¹ dry sediment), EPS carbohydrate concentrations, and prokaryotic 16S rRNA gene copy numbers for all sampling times, elevations, and treatments.

Global identifier:

Doi(
    "10.1594/PANGAEA.994659",
)

DataMeasurements(
    DataMeasurements {
        domain: Unspecified,
        station: None,
        measured_variables: [
            "16S rRNA gene, copy number, per unit sediment dry mass",
            "Chlorophyll a, per unit sediment mass",
            "DEPTH, sediment/rock",
            "Day of experiment",
            "Depth, bottom/max",
            "Depth, top/min",
            "Event label",
            "Extrapolymeric substances",
            "Mesocosm label",
            "Treatment: temperature",
            "Type of study",
        ],
        methods: [
            "Microplate reader, BioTek, Synergy MX",
            "quantitative polymerase chain reaction (qPCR) primer: 519F/907R; LightCycler 480, Roche",
        ],
    },
)

Tags: Ethanol ? Kohlenhydrat ? Pigment ? Wattenmeer ? Deutschland ? Marine Hitzewelle ? Temperatur ? Studie ? Sediment ? Biomasse ? bacterial abundance ? benthic microalgae ? marine heatwaves (MHWs) ? mesocosm experiment ?

Region: Spiekeroog, German Bight, North Sea

Bounding boxes: 7.74874° .. 7.74874° x 53.749384° .. 53.749384°

License: Creative Commons Namensnennung 4.0 (Data access requires registration)

Language: Englisch/English

Organisations

• Carl von Ossietzky Universität Oldenburg, Germany (Mitwirkung) [1]
• Deutsche Bundesstiftung Umwelt (Mitwirkung)
• Deutsche Forschungsgemeinschaft (Finanzielle Förderung)
• GEOMAR - Helmholtz Centre for Ocean Research Kiel (Mitwirkung) [1]
• Helmholtz Institute for Marine Functional Biodiversity, Carl von Ossietzky University Oldenburg, Oldenburg, Germany (Mitwirkung)
• Institute for Chemistry and Biology of the Marine Environment, Carl von Ossietzky Universität Oldenburg (Management, Mitwirkung) [1]
• PANGAEA (Herausgeberschaft)

Persons

• Bert Engelen (Beitragende*r) [ORCID]
• Birte Matthiessen (Beitragende*r) [1] [ORCID]
• Daniela Pieck (Beitragende*r) [1] [ORCID]
• Helmut Hillebrand (Beitragende*r) [1] [ORCID]
• Kertu Lõhmus (Beitragende*r) [1] [ORCID]
• Mike Smykala (Beitragende*r) [1] [ORCID]
• Stefanie Moorthi (Beitragende*r) [1]

Modified: 2026-05-13

Last harvest: 20.05.2026 01:43

Time ranges: 2024-06-26 - 2024-06-26

Resources

• https://doi.pangaea.de/10.1594/PANGAEA.994659?format=textfile (TSV) ?

Status

Aktiv

Quality score

• Overall: 0.85
• Findability: 0.73

  • Title: 0.60
  • Description: 0.31
  • Identifier: true
  • Keywords: 0.71
  • Spatial: Point (0.75)
  • Temporal: true
• Accessibility: 0.67

  • Landing page: Specific (1.00)
  • Direct access: true
  • Publicly accessible: false
• Interoperability: 1.00

  • Open file format: true
  • Media type: true
  • Machine-readable metadata: true
  • Machine-readable data: true
• Reusability: 1.00

  • License: ClearlySpecifiedAndFree (1.00)
  • Contact info: true
  • Publisher info: true

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