Optimization of a Method for the Isolation of Chloroplasts from Fresh Plant Material for the Development of a Bioassay Test for the Determination of Pesticides in Water

Description: Das Projekt "Optimization of a Method for the Isolation of Chloroplasts from Fresh Plant Material for the Development of a Bioassay Test for the Determination of Pesticides in Water" wird/wurde gefördert durch: Kommission der Europäischen Gemeinschaften Brüssel. Es wird/wurde ausgeführt durch: Universität Bonn, Landwirtschaftliche Fakultät, Institut für Landwirtschaftliche Botanik und Landwirtschaftlich-Botanischer Garten.Bio-assays are increasingly used in supplement to classical analyses to determine the effect of contamination of waters with herbicides, some of which have been shown to be able to determine herbicides within the limits in compliance with EC-ordonance for drinking water. Preliminary work carried out at the University of Bonn has demonstrated that contamination of different water systems can be identified using inhibition of the light dependent production of oxygen by chloroplasts. Further experiments at IRMM have shown a potential to transfer membrane systems of chloroplasts into stable powder that can be used to carry out such bio-assays. Results: A method has been developed tor the isolation and breakage ot chloroplasts that allow freeze drying of the thylakoid membranes. The photosynthetic activity of the lyophilized material was maintained to 86 - 95 per cent. This powder can be stored for over five month without loss of activity.

SupportProgram

Origin: /Bund/UBA/UFORDAT

Tags: Schädlingsbekämpfungsmittel ? Herbizid ? Tierhaltungsanlage ? Chloroplasten ? Biomonitoring ? Sauerstoff ? Anlagenoptimierung ? Photosynthese ? Trinkwasseruntersuchung ? Verfahrensoptimierung ? Biotest ? Messverfahren ? Trennverfahren ? Trocknung ? Wassergefährdende Stoffe ? Wasserverschmutzung ? Pflanze ? Tiefkühlung ? Wasser ? Rückstand ? Gewässer ? Schadstoffnachweis ? Thylakoide ? Thylakoidmembran ? Zellfreies-System ?

Region: Nordrhein-Westfalen

Bounding boxes: 6.76339° .. 6.76339° x 51.21895° .. 51.21895°

License: cc-by-nc-nd/4.0

Language: Englisch/English

Organisations

• Commission of the European Communities, Joint Research Centre Geel, Institute for Reference Materials and Measurements (Mitwirkende)
• Kommission der Europäischen Gemeinschaften Brüssel (Bereitsteller*in)
• Umweltbundesamt (Bereitsteller*in)
• Universität Bonn, Landwirtschaftliche Fakultät, Institut für Landwirtschaftliche Botanik und Landwirtschaftlich-Botanischer Garten (Betreiber*in)

Time ranges: 1994-10-01 - 2025-04-25

Status

Aktiv

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