Description: Das Projekt "Culturable and Nonculturable Cells of Pseudomonas Introduced Into Soil for Sustaining Plant Health: Mechanisms and Impact on Ecosystems" wird/wurde gefördert durch: Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung. Es wird/wurde ausgeführt durch: Eidgenössische Technische Hochschule Zürich, Institut für Pflanzenwissenschaften.Leading Questions: - the occurrence of different physiological states of strain CHA0 in soil and in the rhizosphere of crop plants, - environmental factors and bacterial signals influencing culturability and resuscitation of non-culturable cells- the physiological and genetic mechanisms governing culturability vs. Nonculturability. Abstract: Field application of beneficial Pseudomonas bacteria for protection of crops against root deseases or bioremediation of polluted soils implies the release of high numbers of wild-type or genetically-engineered bacterial cells into the soil environment. Since very little is known on the fate of bacterial inoculants in the environment and their impact on the soil ecosystem, concern about the ecological safety of such releases has been raised. The first objective of this project is to assess the persistence of the beneficial bacterium Pseudomonas fluorescens CHA0, which protects the roots of plants from certain soil-borne fungal pathogens, once it is introduced into the soil environment. Particular attention is being paid to the survival of the bacterium as viable but non-culturable cells, because these cannot be monitored by standard methods. We investigate (i) the occurrence of different physiological states of strain CHA0 in soil and in the rhizosphere of crop plants, (ii) environmental factors and bacterial signals influencing culturability and resuscitation of non-culturable cells, and (iii) the physiological and genetic mechanisms governing culturability vs. non-culturability. The second objective of the project is to evaluate, under contained conditions, the ecological impact of strain CHA0 and its derivatives on the diversity of selected indigenous microbial populations and key biological processes in the rhizosphere. Since suppression of diseases by strain CHA0 involves the production of antifungal compounds, it is important to analyse the impact of these compounds on the microorganisms naturally present in the soil environment. Results will help us predict the fate and impact of bacterial inoculants in the environment and improve monitoring of these microorganisms with respect to biosafety.
SupportProgram
Origin: /Bund/UBA/UFORDAT
Tags: Getreide ? Kulturpflanze ? Physiologie ? Pseudomonas ? Feldfrucht ? Bodenmikroorganismen ? Bodenverunreinigung ? Pflanzenwurzel ? Bakterien ? Bodenökosystem ? Bodensanierung ? Bodenschutz ? Gentechnisch veränderte Organismen ? Monitoring ? Nützling ? Ökologische Wirksamkeit ? Pflanzenkrankheit ? Mikroorganismen ? Pilz ? Pflanzengesundheit ? Aufbereitungstechnik ? Krankheit ? Krankheitserreger ? Ökologischer Faktor ? Ökosystem ? Persistenz ? Pflanzenschutz ? Population ? Sanierung ? Kulturstamm-CHA0 ? Pseudomonas-fluorescens ?
License: cc-by-nc-nd/4.0
Language: Englisch/English
Time ranges: 1996-02-01 - 2000-01-31
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