Description: Das Projekt "Phytochelatins and thiol peptides in freshwater algae in response to metal exposure: intracellular and extracellular ligands" wird/wurde gefördert durch: Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung. Es wird/wurde ausgeführt durch: Eawag - Das Wasserforschungsinstitut des ETH-Bereichs.Heavy metals such as cadmium, lead, copper are ubiquitous water pollutants, which may affect aquatic organisms. Metal homeostasis mechanisms mainly involve metal binding proteins that chelate, transport and sequester metals to provide intracellular uptake, distribution and detoxification of metals. Phytochelatins are a class of intracellular metal binding ligands, which are known to be involved in cadmium detoxification in algae. In the present project we aim at examining the role of phytochelatins and of other thiol peptides as metal binding ligands, for the response of freshwater algae to elevated metal exposure and accumulation, and in natural freshwaters. The main objectives of the present project are: (i) to better understand the factors influencing the content of phytochelatins, as well as of other thiols, in response to metal exposure in freshwater algae; (ii) to examine the role of phytochelatins in intracellular sequestration of metals and in their detoxification; (iii) to assess the intracellular distribution of metals in freshwater algae and to examine relationships between metal accumulation, metal partitioning and toxic effects. To answer these questions, experiments with algae cultures exposed to several metals (lead, cadmium, arsenic) will be carried out. The kinetics of formation and elimination of phytochelatins will be determined under various conditions. The intracellular complexes of phytochelatins with these metals will be examined using size exclusion chromatography and electrospray ionization mass spectrometry. Intracellular distribution of metals among phytochelatin complexes and other cytosolic ligands will be studied. The results of this project will provide new insights into the complex interactions between heavy metal inputs in natural freshwaters, biota and natural organic matter. This work contributes to the scientific basis of regulatory issues of heavy metals in natural waters.
SupportProgram
Origin: /Bund/UBA/UFORDAT
Tags: Kupfer ? Peptid ? Thiol ? Blei ? Cadmium ? Textilchemikalien ? Arsen ? Schwermetall ? Brunnen ? Entgiftung ? Organisches Material ? Süßwasser ? Metall ? Chromatografie ? Bildung ? Beschlagnahme ? Bewässerung ? Exposition ? Protein ? Toxikologie ? Toxizität ? Gewässerorganismen ? Wassergefährdende Stoffe ? Algen ? Wasser ? Anreicherung ? Arbeit ? Organismen ? Umwelt ? Gewässer ? Verkehr ? GEGENWART ? VERFRACHTUNG ? FRAGE ? VERHAELTNIS ? BEWERTEN ? Bemessung ? MECHANISMEN ? VERTEILUNG ? Ionisation ? PROJEKT ? ERGEBNIS ? GEMISCHT ? SONSTIG ? UNTER ? WISSENSCHAFTLICH ? GEHALT ? Muskelarbeit ? NEU ? VERANTWORTUNG ?
License: cc-by-nc-nd/4.0
Language: Englisch/English
Time ranges: 2006-07-01 - 2009-06-30
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