Description: Um G. oxydans als Ganzzell-Katalysator ökonomisch einsetzbar zu machen, soll die Produktion von Biomasse in hoher Zelldichte ermöglicht werden. Untersuchungen zur Funktionalität und Regulation der für das Wachstum wichtigen Stoffwechselwege mittels Transkriptom und Proteom Analysen und mittels Ausschalten oder Überexprimieren verschiedener Schlüsselenzyme. Das vorliegende Projekt widmet sich zukunftsorientierten, umweltfreundlichen Verfahren, die mittelfristig die klassischen chemischen Verfahren, z.B. zur Herstellung von Vitamin C und Essigsäure, ablösen werden.
Types:
SupportProgram
Origins:
/Bund/UBA/UFORDAT
Tags:
Wachstumsregulator
?
Essigsäure
?
Ascorbinsäure
?
Katalysator
?
Kohlenstoff
?
Physiologie
?
Vitamin
?
Material
?
Habitat
?
Biotechnologie
?
Chemisches Verfahren
?
Energiequelle
?
Verfahrenstechnik
?
Wirtschaftlichkeit
?
Mikroorganismen
?
Biomasse
?
Stoffwechsel
?
GreenTech
?
Biomasseproduktion
?
Enzym
?
Region:
Nordrhein-Westfalen
Bounding boxes:
6.76339° .. 6.76339° x 51.21895° .. 51.21895°
License: cc-by-nc-nd/4.0
Language: Deutsch
Organisations
Time ranges:
2006-06-01 - 2009-10-31
Alternatives
-
Language: Englisch/English
Title: Growth physiology of Gluconobacter oxydans: entry site of carbon into metabolism and sources of energy
Description: Gluconobacter oxydans strains are useful and versatile catalysts for regioselective oxidations of sugars and sugar alcohols. Much research effort has been focused on membrane-bound enzymes catalyzing these oxidations by channeling the substrate-derived electrons via the respiratory chain to oxygen. The growth physiology of this organism has been investigated to a much lesser extent. Inefficient coupling between energy generation and growth is a characteristic trait of the genus Gluconobacter. Prerequisite for an economical industrial exploitation of G. oxydans as a whole-cell catalyst is the production of cell mass under retention of the organisms capability for periplasmatic regioselective oxidation. Aim of the work is the elucidation of the reasons of extracellular accumulation of intermediates during glucose oxidation catalyzed by membrane-bound dehydrogenases. Since this accumulation of intermediates influences growth as well as product yields, the planned work will contribute to a better understanding of the coupling of the energy yielding, membrane associated and energy consuming, cytoplasmic pathways. The studies will comprise transcription analyses of the effects of glucose and gluconate on the expression of the PPW and ED genes, in vivo and in vitro inhibition studies of the membrane bound sorbitol dehydrogenase and determination of the P/O ratios for glucose, gluconate and ketogluconates for membrane associated and cytoplasmic pathways. Further studies will examine the function of cytochrome C in the respiratory chain of G. oxydans.
https://ufordat.uba.de/UFORDAT/pages/PublicRedirect.aspx?TYP=PR&DSNR=1007846
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