The studied core CON01-603-2 was recovered from the Continent site, Northern Basin from a water depth of 386 m (Fig. 1) (see Charlet et al., 2005-this volume). The analysed sequence (725.5–608 cm) consists of mainly of biogenic, diatomaceous sediments, although the upper part of the sequence between ca. 611–608 cm contains more silt particles and less diatoms than the lower part of the sequence. From a depth of 690 cm upwards the sediments are finely and coarsely laminated.Based on a standard technique for processing palynological samples, silicates were removed from the sediment by treatment with 40% HF for 3 days and with 50% HF for 1 day. Following Erdtmans acetolysis (Faegri and Iversen, 1989) sediment samples were sieved through 7-µm meshes in an ultrasonic water bath (Cwynar et al., 1979).
Sediment slices of 0.5 cm thickness were obtained from gravity core segments and of 1 cm thickness from the Vydrino piston core. Volumetric subsamples of 5 cm3 (10 cm3 in case of the lowermost samples from Continent core) were prepared according to standard procedures, including 7-μm ultrasonic fine-sieving (Cwynar et al., 1979, Fægri et al., 1989 K. Fægri, P.E. Kaland and K. Krzywinski, Textbook of Pollen Analysis (4th edition), John Wiley & Sons, Chichester (1989) 328 pp..Fægri et al., 1989 and PALE Steering Committee, 1994). Two tablets of Lycopodium marker spores were added to each sample for calculating total pollen and spore concentrations (Stockmarr, 1971). Water-free glycerol was used for storage and preparation of microscopic slides. The palynological samples were counted at magnifications of 400–600×, applying 1000× for the identification of difficult pollen types, e.g., including Saxifragaceae, Crassulaceae, and Rosaceae.
Sediment slices of 0.5 cm thickness were obtained from gravity core segments and of 1 cm thickness from the Vydrino piston core. Volumetric subsamples of 5 cm3 (10 cm3 in case of the lowermost samples from Continent core) were prepared according to standard procedures, including 7-μm ultrasonic fine-sieving (Cwynar et al., 1979, Fægri et al., 1989 K. Fægri, P.E. Kaland and K. Krzywinski, Textbook of Pollen Analysis (4th edition), John Wiley & Sons, Chichester (1989) 328 pp..Fægri et al., 1989 and PALE Steering Committee, 1994). Two tablets of Lycopodium marker spores were added to each sample for calculating total pollen and spore concentrations (Stockmarr, 1971). Water-free glycerol was used for storage and preparation of microscopic slides. The palynological samples were counted at magnifications of 400–600×, applying 1000× for the identification of difficult pollen types, e.g., including Saxifragaceae, Crassulaceae, and Rosaceae.
Sediment slices of 0.5 cm thickness were obtained from gravity core segments and of 1 cm thickness from the Vydrino piston core. Volumetric subsamples of 5 cm3 (10 cm3 in case of the lowermost samples from Continent core) were prepared according to standard procedures, including 7-μm ultrasonic fine-sieving (Cwynar et al., 1979, Fægri et al., 1989 K. Fægri, P.E. Kaland and K. Krzywinski, Textbook of Pollen Analysis (4th edition), John Wiley & Sons, Chichester (1989) 328 pp..Fægri et al., 1989 and PALE Steering Committee, 1994). Two tablets of Lycopodium marker spores were added to each sample for calculating total pollen and spore concentrations (Stockmarr, 1971). Water-free glycerol was used for storage and preparation of microscopic slides. The palynological samples were counted at magnifications of 400–600×, applying 1000× for the identification of difficult pollen types, e.g., including Saxifragaceae, Crassulaceae, and Rosaceae.
Sediment slices of 0.5 cm thickness were obtained from gravity core segments and of 1 cm thickness from the Vydrino piston core. Volumetric subsamples of 5 cm3 (10 cm3 in case of the lowermost samples from Continent core) were prepared according to standard procedures, including 7-μm ultrasonic fine-sieving (Cwynar et al., 1979, Fægri et al., 1989 K. Fægri, P.E. Kaland and K. Krzywinski, Textbook of Pollen Analysis (4th edition), John Wiley & Sons, Chichester (1989) 328 pp..Fægri et al., 1989 and PALE Steering Committee, 1994). Two tablets of Lycopodium marker spores were added to each sample for calculating total pollen and spore concentrations (Stockmarr, 1971). Water-free glycerol was used for storage and preparation of microscopic slides. The palynological samples were counted at magnifications of 400–600×, applying 1000× for the identification of difficult pollen types, e.g., including Saxifragaceae, Crassulaceae, and Rosaceae.