Although the use of genetically modified plants for bioremediation, or the in situ cleaning of contaminated sites, has been known for quite some time, little attention has so far been paid to the production of antibodies in plants and their ex vivo application in selective depletion. Therefore, highly affine and specific antibodies against algal toxins using microcystin as an example will be produced in plants at low cost within this research project. The basis is a monoclonal antibody (Mab 10E7, species: mouse) generated in a former research project. The sequence of the variable domains will be determined, optimized for plants and sub cloned into suitable plant transformation vectors, which already contain constant antibody sequences. In addition, a scFv fragment containing different tag sequences and fusion proteins will be constructed. Leaf-based (tobacco) as well as seed-based (barley) systems will be used.Affinity-purified plant-produced antibodies (plantibodies) will be characterized in detail for their binding properties using microtitre plate-ELISA and surface plasmon resonance (SPR). The monoclonal mouse antibody will be used as reference. To assure cost-efficiency for future applications, roughly purified fractions (sequential pH and temperature treatment followed by filtration) will be tested for the upscaling. Following immobilization of the plantibody fractions on suitable substrates, for instance membranes, porous polymer monoliths or in porous glasses, their application for depletion will be defined using model water samples spiked fortified with microcystins.
In strict nature reserves and core zones of protected areas hunting and forestry operations are often restricted or banned. However, regarding the management of Wild boar, such hunt-free zones are discussed controversially and can lead to conflict. Hunters whose areas border no-hunting zones (and who have to reimburse farmers for crop damages caused by Wild boar) are concerned that the boars may evade effective population management by staying within the limits of the no-hunting zone, and farmers fear increased crop damage in the surroundings of such areas. Some conservationists are also concerned because Wild boars increasingly root protected habitats and can cause damage to rare plant assemblies. The three-year project Wild boar problem in the vicinity of protected areas by the Game Research Institute (Wildforschungsstelle) at the Centre for Agriculture Baden-Württemberg (LAZBW) aims at investigating if and how no-hunting zones might affect Wild boar activity, movement patterns, home range size, and habitat use, as well as crop damage caused by boars, by comparing these aspects between hunting-free zones and unprotected areas. Although there have already been a number of telemetry studies on Wild boar, including space use in the context of hunting activity, to date there is no study that has specifically investigated spatial and ecological aspects in and around protected areas. My dissertation Ecology of Wild boar Sus scrofa in the vicinity of protected areas is being carried out within the scope of the Game Research Institutes project and apart from the aims outlined above, further aspects of Wild boar ecology will be investigated, especially the role of Wild boar as bio-engineer and habitat creator for other species vs. unwanted damages at protected sites. Twenty-seven Vectronic GPS-GSM satellite collars with integrated activity sensors are available to tag Wild boars in three study areas: the non-protected Altdorfer Forest near Aulendorf with regular hunting activity and forestry, the nature reserve Wurzacher Ried with its ca. 700 ha core zone that is a strict reserve with no human activity, and the Biosphere Reserve Swabian Jura, especially in the surroundings of the former military training area near Münsingen and the 170 ha no-usage-area Föhrenberg.
Climate conditions along the Antarctic Peninsula have considerably changed in the last 50 years. The glaciers on the Western Antarctic Peninsula (WAP) have already shown reactions of change by speedup and surface lowering. The disintegration of the Larsen-A and B Ice Shelves, the ice shelves in the Larsen Inlet, Prinz-Gustav-Channel and Wordie Ice Shelf have led to a surge-type behaviour of tributary glaciers to which much of the current contribution of Antarctic Peninsula ice to sea level rise is attributed. However, quantifications of mass loss from the peninsula using different observations and methods are still ambiguous. We propose to improve the quantifications of mass loss in the area of the former Northern Larsen-A embayment as well as for Western Antarctic Peninsula glaciers including tributaries to former Wordie Ice Shelf. In order to achieve those goals we analyse time series of SAR satellite data from the ESA archive to determine glacier velocity changes for these regions over the last 20 years. We use data from the new national mission TerraSAR-X and TanDEM-X to generate ice velocity fields, to determine surface elevation changes and to map the grounding line of tidewater glaciers. We will back our products with ground truth measurements from two field campaigns and laser altimetry from collaborating partners. In an integrated analysis we link those data sets to achieve a better glaciological understanding of underlying processes. We apply a novel approach to quantify volume changes and to derive ice thickness maps for selected areas using a mass conserving approach that leverages on the various remote sensing data sets generated.
In my Phd-study, I examine the resource conflict over industrial logging in the South Moresby area on Haida Gwaii (former Queen Charlotte Islands) from 1974 to 1993. On this remote archipelago on British Columbias west-coast, a fierce dispute over logging practices, land rights, Aboriginal land claims and environmental issues had emerged in the face of devastating logging practices were not only threatening to destroy large parts of Moresby Island but also resulted in the destruction of salmon streams. The two major natural resources available on the islands were at stake during the 'war in the woods.' Together with environmentalist, the Haida First Nation successfully fought for the preservation of Gwaii Haanas (South Moresby Island) with blockades, protests, environmental campaigns, lobbying and legal action. The area is now protected as the 'Gwaii Haanas National Park Reserve.-' Using a cultural approach combining aspects of 'storytelling,' the 'cultural memory' and 'actor-network-theory' (ANT), the core question arises whether the (Western) dualisms between 'nature' and 'culture' and between 'fact' and 'fiction' should be dissolved. Latour argues that such dualisms and even the separation between the human and non-human world (i.e., the world of things) do not exist. Assmann claims that we do not necessarily remember what has really happened', but what was repeatedly told us to have happened. Along with Thomas King, J. Edward Chamberlin and William Cronon, I stress the point that stories - fictitious or based on facts - do matter. The effect of stories on the way we understand our past, might often be more important than that of 'hard' facts: Stories shape both a societys concept of the past and its present identity. Canada, with its heterogeneous population, is a particularly interesting place to conduct research about different concepts of 'nature' and culture'. Analyzing the different stories that evolved around South Moresby, and taking into account diverging messages of native and non-native stories, offers a new perspective on similar resource conflicts that continue to exist and arise all over the world.
The HyTEC project will expand the existing European network of hydrogen demonstration sites into two of the most promising early markets for hydrogen and fuel cells, Denmark and the UK. The capital cities of Copenhagen and London will deploy a fleet of 30 next generation hydrogen fuel cell passenger vehicles in three different vehicle classes taxis, passenger cars and scooters. These state-of-the-art vehicles will be used in day to day urban fleet operations, thus taking a step forward from technology demonstration projects, towards fuel cell vehicles in the hands of real users. New publicly accessible fuelling stations will lead to genuine refueling networks covering each capital city. These will be appropriate for expanding the pre-commercial rollout of hydrogen vehicles. An extensive research program will assess the technical performance of the vehicles over a two year demonstration period, a life cycle impact assessment of the vehicles will demonstrate the overall well to wheels impacts and social studies will investigate the non-technical barriers. The results of the project will inform future commercialization and infrastructure rollout planning by policy makers and industry players. A widespread dissemination campaign will be aimed at improving public awareness of the potential for hydrogen vehicles as a future low carbon transport solution. This will be backed up by a more targeted campaign aiming at industry players, opinion formers and key policy makers across Europe. For the first time, the project will create genuine links between the new and existing European hydrogen demonstration projects, with a view to informing ongoing strategic planning for hydrogen rollout and also ensuring a common voice towards the expansion of the hydrogen vehicle fleet in Europe towards commercialization.
Large areas of abandoned pasture land in the Alps are currently encroached by shrub at a breathtaking speed, with green alder (Alnus viridis) playing the most prominent role. Dense Alnus thickets reduce plant diversity in former species-rich upper montane grassland, prevent natural forest succession, change the water relations at the landscape scale by reducing runoff, contribute through symbiotic N2 fixation substantially to eutrophication by leaching nitrate to the river system and exert risks to local drinking water springs. Paradoxically, this conversion into species-poor, nitrogen enriching and releasing Alnus thickets occurs in mountain regions considered particularly rich in biodiversity and essential for the continuing provision of many ecosystem goods and services, particularly for clean and plentiful water. Except from local sources, critical loads for nitrogen by atmospheric deposition are only rarely exceeded in these high elevation regions. The project ALNEX is designed (1) to quantify the water quality impact of Alnus by measuring the nitrogen pools and fluxes from single alder shrub to catchment-wide landscapes, (2) to elaborate land management guidelines towards preserving of open, centuries-old pasture land and (3) to assess the policy implications in a critical load and biodiversity context.
Arbuscular- (AM) and ectomycorrhizal (EM) fungi influence soil organic matter (SOM) quantity and quality using different mechanisms. So far little is known how changes in the dominating mycorrhiza type occurring after land use change from arable crops to short rotation forestry (SRF) affect the quantity, composition and turnover of SOM. Therefore, we will manipulate the dominating mycorrhiza type in field and lab experiments to test the following hypotheses: (1) A change from AM to EM, expected under SRF, results in SOM storage due to mycelium formation and SOM stabilising hydrophobin production; (2) A back-change to AM in crops after SRF stimulates saprotrophs to decompose the previously stored SOM and forms new SOM using aggregating effects of glomalin. Accordingly, we will sample recent and former long term SRF and their corresponding reference sites. We will investigate the mycorrhizal colonisation, the amount and molecular composition of SOM including markers (chitin, hydrophobin, glomalin). The microbial decomposition of SOM in the presence and absence of EM will be investigated using 13C studies in incubation experiments. This will prove the importance and sustainability of mycorrhizal C for SOM formation.
Implementation of EU waste legislation shows large differences in the EU Member States especially with regard to municipal waste management. Major discrepancies prevail particularly in the implementation and application of the Waste Framework Directive and proper transposition of EU requirements into national legislation. The waste management performance of all EU Member States was subject to screening to identify those Member States with the largest implementation gaps, in particular in relation to municipal waste management. For screening the main elements and legal requirements stemming from EU waste directives (mainly from the Waste Framework and the Landfill Directive) were considered for the design of suitable criteria. These core elements comprise the practical implementation of the waste management hierarchy, application of economic and legal instruments to move up the waste hierarchy, sufficiency of treatment infrastructure and quality of waste management planning, the fulfilment of targets and infringement procedures. These elements were assessed by 18 criteria for each Member State taking into account information sources at EU, national or regional level. Latest available statistical data and data of former years for comparison of development within a country were extracted from the EUROSTAT database. References comprised reports published by the European Commission, the European Topic Centre on Sustainable Consumption and Production, internal working documents of EUROSTAT and the EU Commission as well as national/regional Waste Management Plans. Where available also Waste Prevention Programmes were screened. The screening results confirmed the assumption of large differences within the EU-27 with regard to treatment of municipal waste, compliance with the WFD and Landfill Directives and application of legal or economic instruments as well as planning quality. For each criterion two, one or zero points could be achieved, leading to maximum points of 42 for all criteria. The methodology includes weighting of results for three selected criteria related to the application of the treatment options recycling, energy recovery and disposal of municipal waste.
IAGOS is a design study pursuing the preparation of a resilient distributed infrastructure for routine observations of atmospheric composition, aerosols, clouds and contrails on the global scale from commercial in-service aircraft. Observations in the Up per Troposphere and Lower Stratosphere (UTLS) are critical for improving the scientific understanding of chemistry-climate interactions, particularly those associated with the roles of clouds, aerosols and chemical composition. This information is essen tial for improving the scientific basis related to predictions of global climate change and for the assessment of surface air pollution, including the influence of aviation impacts and of emissions from other parts of the world on Europe. In IAGOS, new instrument packages will be developed which include state of the art developments based on the former MOZAIC instrumentation for O3, H2O, CO and NOy/NOx with significant reductions in size and weight. A central element is the certification of the packa ges for installation and deployment on Airbus longrange aircraft and for maintenance in compliance with aeronautical regulations. New instrumentation will be designed for aerosol, cloud particles and for stratospheric water vapour. Another important elem ent is the design of realtime data provision from the new instruments to meteorological services. Finally, IAGOS will establish the logistic and financial boundary conditions for the operation of the new infrastructure and will initiate the dialog betwe en scientific partners, users and airlines interested in supporting the new infrastructure. IAGOS is epected to make a significant step forward in the development of a globally operated in situ ob-servation network for the climate system.
Within the project 'Causes and effects of natural and man-made soil degradation in Bashkortostan - basic investigations for sustainable agricultural management strategies in semiarid regions (project manager Prof. Dr. M. Frühauf , Institute of Geosciences, workgroup Geoecology, Martin-Luther University of Halle-Wittenberg), different tasks and theses should be developed for PhD- qualification. Main topics are the development and evaluation of indicators of soil degradation in the forest steppe zone of Bashkortostan. In this process measurements for quantification of soil degradation and soil water balance under different land use will take place. Natural soils of the forest steppe zone of Bashkortostan will act as reference. Furthermore relationships between climate parameters, soil properties, soil water budgets/ dynamics and yield development will be investigated. Developing of a GIS structure gather the main causal connections between land use, soil degradation patterns and soil erosion. In addition spatial and temporal analyses of yield development and soil quality will describe. It is note one of the most important tasks will be the regionalization from farm scale to the rayon level as step towards the regionalization of the whole forest steppe zone. These activities should be a contribution to development of regionally adapted strategies for soil protection and sustainable land use.
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