Das Projekt "Glufosinat: Metabolismus in transgenen und nicht-transgenen Pflanzengeweben sowie Schicksal im Boden" wird vom Umweltbundesamt gefördert und von RWTH Aachen University, Institut für Umweltforschung, Biologie V, Lehrstuhl für Umweltbiologie und -chemodynamik durchgeführt. Glufosinat (oder Phosphinotricin) ist ein vergleichsweise modernes Herbizid, das seit etwa 25 Jahren in Gebrauch ist. Bei der Verbindung handelt es sich um eine Aminosäure; üblicherweise bezeichnet man das DL-Racemat als Glufosinat, das L-Enantiomer als Phosphinothricin. Die Verbindung ist Teilstruktur eines von den Pilzen Streptomyces viridochromogenes und Streptomyces hygroscopicus produzierten natürlichen Antibiotikums (Tripeptid: L-Alanin-L-Alanin-L-Phosphinothricin). Neben seiner antibakteriellen Wirkung zeigt Glufosinat eine nicht-selektive herbizide Wirkung. Der antibakterielle und herbizide Effekt geht nur vom L-Enantiomer aus; das D-Enantiomer ist inaktiv. Sowohl Glufosinat (Racemat) als auch das Tripeptid (Bialaphos oder Bilanaphos; mit L-Enantiomer) werden als Herbizide vermarktet. Die herbizide Wirkung von Phosphinothricin beruht auf einer Inhibition der Glutaminsynthetase. Glufosinat weist günstige ökotoxikologische Eigenschaften auf, z.B. bezüglich Versickerung, Abbau sowie Toxizität gegenüber Tier und Mensch. Auf Grund dieser Eigenschaften ist Glufosinat ein geeigneter Kandidat zur Herstellung gentechnisch modifizierter Herbizid-resistenter Pflanzen, um Glufosinat auch selektiv - im Nachauflauf - einsetzen zu können. Dazu wurden verschiedene Spezies, wie z.B. die Zuckerrübe, mit dem bar-Gen aus Streptomyces hygroscopicus transformiert. Das bar-Gen codiert für eine Phosphinothricin-N-acetyltransferase, die Phosphinothricin zum nicht herbizid-wirksamen, stabilen N-Acetylderivat umsetzt. Bei entsprechend hoher Expression des bar-Gens resultiert eine Glufosinat-resistente Pflanze. Ein Ziel unseres Forschungsvorhabens war es, den Metabolismus von Glufosinat und der einzelnen Enantiomere (L- und D-Phyosphinothricin) in transgenen und nicht transgenen Pflanzenzellkulturen zu untersuchen. Die transgenen Kulturen, die von der Zuckerrübe (Beta vulgaris) stammten, waren mit dem bar-Gen transformiert, exprimierten demnach die Phosphinothricin-N-acetyltransferase. Sie wurden aus entsprechenden Sprosskulturen initiiert. Daneben wurden nicht-transgene Kulturen von Zuckerrübe, Karotte (Daucus carota), Fingerhut (Digitalis purpurea) und Stechapfel (Datura stramonium) untersucht. In einer zweiten Versuchsserie wurden abgetrennte Sprosse und Blätter von 20 Wildpflanzen auf den Metabolismus von Glufosinat untersucht. Es sollte überprüft werden, ob qualitative und quantitative Unterschiede im Umsatz des Herbizids im Pflanzenreich vorkommen und möglicherweise eine natürliche (teilweise) Resistenz gegenüber Glufosinat existiert. Schließlich wurde das Schicksal des Herbizids im Boden (Abbau, Versickerung) nach Aufbringung des Wirksstoffs in einer handelsüblichen Formulierung auf ein bewachsenes Versuchsfeld im Freiland untersucht.
Das Projekt "The European aeroemissions network (AERONET)" wird vom Umweltbundesamt gefördert und von Deutsches Zentrum für Luft- und Raumfahrt e.V., Institut für Antriebstechnik durchgeführt. One of the major problems that civil aeronautics will have to face over the next twenty or thirty years is to accommodate the predicted growth in demand of air transport without creating unacceptable adverse environmental effects. It is to be expected that new scientific results, increasing public concerns over the environment and future restrictive regulations with respect to aircraft emissions will force airline companies to take ecological considerations much more into account than it does at present. Consequently, for European aircraft manufacturers it is of high importance to react early and to guide their research and development resources into the most important and efficient direction. The aim of the AERONET project is to support coordination ' a postiori' of existing European and national projects or programmes dealing with the contribution of air traffic emissions to anthropogenic climate and atmospheric changes. For this purpose AERONET seeks to : - bring together experts from engine technology, atmospheric research and operations as well as programme responsible to exchange knowledge and opinions and to discuss necessary future actions on the basis of jointly defined goals and time scales, - produce competitive advantage for Europe through enhanced information echoing in the field of atmospheric effects of air traffic emissions, - strengthen a common European position in global technical and political discussions - support the Commission in identifying topics for the 5th Framework Programme, - identify gaps and help prepare a coordinated submission of proposals. European Dimension and Partnership: Europe is, beside the US, one of the two biggest aircraft manufacturers. One supposition for the economic success of European aircraft industry is not only to fulfill the existing regulations but, due to the long development times of 5-10 years and the long lifetimes of aircraft of more than 20 years, also to take the trend of future regulations development into account at a very early stage. This needs continuous and fast information exchange and discussions between atmospheric scientists, aircraft engineers and regulatory organisations. To be successful with an effort of this dimension, optimal coordination of national and European programmes in all three fields is required. Thus the network brings together representatives of all programmes and institutions concerned, helps to integrate activities through better information exchange, tries to identify the most urgent themes for R&D activities and intends to give recommendations for the Fifth Framework Programme. Potential Applications: Understanding the atmospheric impacts, the technical consequences and development perspectives, and the operational impacts as a whole is absolutely necessary to strengthen the European position in global regulatory committees on the on side and to gain competitive advantages for the European aircraft and airline industries on the other side. usw
Das Projekt "Effect of weed management strategies on the risk of enteric pathogen transfer into the food chain and lettuce yield and quality" wird vom Umweltbundesamt gefördert und von Universität Bonn, Institut für Organischen Landbau durchgeführt. The risk of pathogen transfer from soil to plant, here: lactuca sativa var. capitata, under organic farming conditions is to be investigated within the scope of the QLIF project. When brute fertilisers are applied during production, a health risk by consuming raw eadibles, as e.g. lettuce, is often discussed because of the demanding high standard of sanitation. The type of fertiliser might promote transfer of Enterobacteriaceae, and among these possibly human pathogens. Splash-effects during rainfall and irrigation as well as transfer of soil particles during mechanical weed control. Risks of the pathogen transfer into lettuce will be examined by use of different fertilisation and weed control management strategies, the latter being compared regarding their effectiveness in reducing pathogen transfer. Different field trials with organic fertilisation will be performed in 2006 and 2007. The contents of Enterobacteriaceae, coliforms and E. coli are used as sanitation indicators for the assessment of the effectivity of weed control strategies. Therefore, the contents will be measured in soil as well as in plants. Furthermore, the quality of lettuce will be acquired by analyses of nutrient composition and morphological measurements.
Das Projekt "Wirkung von Mykorrhiza auf das Wachstum von Bäumen in verschiedenen Substraten - Folgeversuch" wird vom Umweltbundesamt gefördert und von Bayerische Landesanstalt für Weinbau und Gartenbau, Abteilung Landespflege durchgeführt. In zunehmende Maße werden Mykorrhiza-Präparate zur Verbesserung des Pflanzenwachstums angeboten. Jedoch fehlen experimentell abgesicherte Ergebnisse über die Wirksamkeit weitgehend im deutschsprachigen Raum. Dieser Versuch ist der Folgeversuch zu Nr. L015 05. Die im Rahmen dieses Versuchs in Töpfen kultivierten Gehölze wurden ausgepflanzt und z.T. erneut oder erstmalig mit Mykorrhiza geimpft. Der Versuch soll zeigen, ob die mit Mykorrhiza geimpften Baumarten ein besseres Wachstum zeigen, insbesondere unter Stress. Wurden die Gehölze im vorangegangenen Versuch L015 05 in Töpfen kultiviert, sind sie in der zweiten Stufe ausgepflanzt und z.T. erneut oder erstmals mit Mykorrhiza geimpft worden. Unter den deutlich erschwerten Standortbedingungen sollten sich deutlichere Unterschiede zwischen den mit Mykorrhiza geimpften und den nicht geimpften Varianten zeigen als im vorangegangenen Gefäßversuch.
Das Projekt "Impact of transgenic crops on fertility of soils with different management history" wird vom Umweltbundesamt gefördert und von Forschungsinstitut für biologischen Landbau Deutschland e.V. durchgeführt. What impact does transgenic maize have on soil fertility? Among the factors that determine soil fertility is the diversity of the bacteria living in it. This is in turn affected by the form of agriculture practiced on the land. What role do transgenic plants play in this interaction? Background Soil fertility is the product of the interactions between the parental geological material from which the soil originated, the climate and colonization by soil organisms. Soil organisms and their diversity play a major role in soil fertility, and these factors can be affected by the way the soil is managed. The type of farming, i.e. how fertilizers and pesticides are used, has a major impact on the fertility of the soil. It is known that the complex interaction of bacterial diversity and other soil properties regulates the efficacy of plant resistance. But little is known about how transgenic plants affect soil fertility. Objectives The project will investigate selected soil processes as indicators for how transgenic maize may possibly alter soil fertility. The intention is in particular to establish whether the soil is better able to cope with such effects if it contains a great diversity of soil bacteria. Methods Transgenic maize will be planted in climate chambers containing soils managed in different ways. The soil needed for these trials originates from open field trials that have been used for decades to compare various forms of organic and conventional farming. These soils differ, for example, in the way they have been treated with pesticides and fertilizers and thus also with respect to their diversity of bacteria. The trial with transgenic maize will measure various parameters: the number of soil bacteria and the diversity of their species, the quantity of a small number of selected nutrients and the decomposition of harvest residues. It will be possible to conclude from this work how transgenic plants affect soil fertility. Significance The project will create an important basis for developing risk assessments that incorporate the effects of transgenic plants on soil fertility.
Das Projekt "Forest management in the Earth system" wird vom Umweltbundesamt gefördert und von Max-Planck-Institut für Meteorologie durchgeführt. The majority of the worlds forests has undergone some form of management, such as clear-cut or thinning. This management has direct relevance for global climate: Studies estimate that forest management emissions add a third to those from deforestation, while enhanced productivity in managed forests increases the capacity of the terrestrial biosphere to act as a sink for carbon dioxide emissions. However, uncertainties in the assessment of these fluxes are large. Moreover, forests influence climate also by altering the energy and water balance of the land surface. In many regions of historical deforestation, such biogeophysical effects have substantially counteracted warming due to carbon dioxide emissions. However, the effect of management on biogeophysical effects is largely unknown beyond local case studies. While the effects of climate on forest productivity is well established in forestry models, the effects of forest management on climate is less understood. Closing this feedback cycle is crucial to understand the driving forces behind past climate changes to be able to predict future climate responses and thus the required effort to adapt to it or avert it. To investigate the role of forest management in the climate system I propose to integrate a forest management module into a comprehensive Earth system model. The resulting model will be able to simultaneously address both directions of the interactions between climate and the managed land surface. My proposed work includes model development and implementation for key forest management processes, determining the growth and stock of living biomass, soil carbon cycle, and biophysical land surface properties. With this unique tool I will be able to improve estimates of terrestrial carbon source and sink terms and to assess the susceptibility of past and future climate to combined carbon cycle and biophysical effects of forest management. Furthermore, representing feedbacks between forest management and climate in a global climate model could advance efforts to combat climate change. Changes in forest management are inevitable to adapt to future climate change. In this process, is it possible to identify win-win strategies for which local management changes do not only help adaptation, but at the same time mitigate global warming by presenting favorable effects on climate? The proposed work opens a range of long-term research paths, with the aim of strengthening the climate perspective in the economic considerations of forest management and helping to improve local decisionmaking with respect to adaptation and mitigation.
Das Projekt "Pasture rehabilitation on, and management of degraded areas in the Andes of South Ecuador" wird vom Umweltbundesamt gefördert und von Universität Bayreuth, Fachgruppe Biologie, Bayreuther Zentrum für Ökologie und Umweltforschung (BayCEER), Lehrstuhl für Pflanzenphysiologie durchgeführt. Project abstract: This knowledge transfer project will be centered in the San Francisco valley in the South Ecuadorian Andes. However, the problem of abandoning pastures because of heavy infestation by weeds, in particular by bracken fern (Pteridium spec.), is a general issue in the tropical Andes. Pastures which have been abandoned for that reason amount meanwhile to 11Prozent of the area of the San Francisco valley. Infestation by bracken fern and shrubs is a consequence of the traditional use of fire for clearing of the natural forest and pasture management. Growth of both, bracken and woody weeds, is fostered by recurrent burning. In a 2-phase experiment on a heavily bracken-infested slope at c. 2000 m altitude, substantial control of the weed and subsequent pasture rehabilitation could be achieved. In the planned project, this procedure shall be scaled-up to farm level and the altitudinal range of repasturisation shall be extended from 1000 m to 2400 m altitude. To that end local farmers will put respective parts of their land to the projects disposal and public authorities will provide man-power. There are several challenges to be met: (i) Long-term bracken infestation has depleted the soils from nutrients, in particular P and N. Therefore targeted fertilization is needed for profitable grass productivity. (ii) Since bracken can never be completely eradicated, its regrowth must be suppressed by trampling, i.e. frequent grazing. A sustainable grazing management has to be developed which corresponds to soil fertility. (iii) The dominating pasture grass is the C4-type grass Setaria sphacelata. It is growing well in a warm climate but its competitive strength in the harsher climate above 2000 m is low. Bracken as a C3-type plant is less dependent on the temperature. To this adds that it occurs in 2 species in the area, one of which is an upland type. Thus the climate gradient over the elevational transect will influence the competitive strength of both competitors. Therefore the suitability of the traditional monoculture of this grass species for pasture rehabilitation shall be tested in the context of a comprehensive pasture management experiment which the farmers will be involved. (iv) A special problem is the high oxalate concentration in the growing parts of the Setaria leaves which can cause calcaemia in cattle. In a pot experiment which will be run in collaboration with a research team of the UTPL, feeding quality and toxicity of a monotonous diet of Setaria will be tested. Beside the experimental areas, demonstration sites will be installed where regular training workshops will be organized to encourage the farmers to apply the developed rehabilitation and management strategy to their own farms.
Das Projekt "Biopores in the subsoil: Formation, nutrient turnover and effects on crops with distinct rooting systems (BioFoNT)" wird vom Umweltbundesamt gefördert und von Universität Bonn, Institut für Organischen Landbau durchgeführt. Perennial fodder cropping potentially increases subsoil biopore density by formation of extensive root systems and temporary soil rest. We will quantify root length density, earthworm abundance and biopore size classes after Medicago sativa, Cichorium intybus and Festuca arundinacea grown for 1, 2 and 3 years respectively in the applied research unit's Central Field Trial (CeFiT) which is established and maintained by our working group. Shoot parameters including transpiration, gas exchange and chlorophyll fluorescence will frequently be recorded. Precrop effects on oilseed rape and cereals will be quantified with regard to crop yield, nutrient transfer and H2-release. The soil associated with biopores (i.e. the driloshpere) is generally rich in nutrients as compared to the bulk soil and is therefore supposed to be a potential hot spot for nutrient acquisition. However, contact areas between roots and the pore wall have been reported to be low. It is still unclear to which extent the nutrients present in the drilosphere are used and which potential relevance subsoil biopores may have for the nutrient supply of crops. We will use a flexible videoscope to determine the root-soil contact in biopores. Nitrogen input into the drilosphere by earthworms and potential re-uptake of nitrogen from the drilosphere by subsequent crops with different rooting systems (oilseed rape vs. cereals) will be quantified using 15N as a tracer.
Das Projekt "Plant-soil interactions in changing rice cropping systems and their influence on C and N dynamics" wird vom Umweltbundesamt gefördert und von Universität Bonn, Institut für Nutzpflanzenwissenschaften und Ressourcenschutz - Pflanzenernährung (Prof. Werner) durchgeführt. Plant-soil interactions drive the input, cycling and losses of C and N in soil. This subproject aims at elucidating the input and fate of C in the soil-plant systems and its effect of N retention in soil under different paddy management (continuous vs. alternating with maize cropping). In particular we will investigate (i) how much of the assimilate C is released by the plants into the rhizosphere soil, and how this rhizodeposition is affected by N supply, soil density and crop variety during plant development, (ii) how the exudation of C and N responds to land use change, (iii) how C released into the rhizosphere affects the turnover of soil C and utilization of fertilizer N, and (iv) to what degree leaching contributes to the loss of C and N from the rooted surface soil. To answer these questions, we will combine the use of isotopic 13C and 15N labeling in laboratory and field experiments with a sophisticated characterization of root exudates, root border cells, and compound-specific isotope tracing in the residues of bacteria and fungi in rhizosphere, bulk soil as well as within different dissolved organic and inorganic carbon species in soil leachates. In this way and in collaboration with SP 2, 5, 6, and 7 of this research unit, our project links the cycling of C and N in paddy soils to one of its most prominent drivers, the release of organic compounds by roots.
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