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OPTIBIOCAT is a 48 months project aimed at developing biocatalysts based on feruloyl esterases (FAEs) and glucuronoyl esterases (GEs) for production of phenolic fatty- and sugar- esters with antioxidant activity for cosmetic industry, expanding the number/type of industrial biotransformations. Selected FAEs and GEs available within the consortium will be improved for their thermo- and solvent- resistance and substrate specificity by site-directed mutagenesis and directed evolution. Novel enzymes will be discovered by mining for new genes from available genomes. An inventory of novel FAEs and GEs will be developed including 50 fungal and 500 bacterial esterases, 25 site-directed and 20 directed evolved mutants. Enzymatic performances will be optimized to enhance the yield (up to the theoretical yield of 100%) and productivity (up to 0.5-1 g/l/h) of reactions giving the main targeted antioxidants: butyl ferulate, p-coumarate, caffeate, sinapate and 5-O-(trans-feruloyl)-arabinofuranose (using FAEs), glucuronate and benzyl glucuronate (using GEs). FAEs and GEs will be also tested for production of other compounds with improved biological activity and properties of hydrophilicity/hydrophobicity for cosmetic applications. Cost-effective methods will be developed for production of the new biocatalysts, in the g/L scale, and for their technical application to produce antioxidants for cosmetic industry, up to 20L. Enzyme immobilization will increase their recyclability up to ten cycles. The ability of the developed catalysts to work in conditions miming the industrial ones with reduced use of solvents and lower temperature than the chemical routes will be demonstrated. The techno-economic viability and environmental friendliness will be assessed considering a full industrial scale scenario. OPTIBIOCAT involves a highly skilled and multidisciplinary partnership of 16 partners from 8 EU countries, and it is a strongly industry driven project through the participation of 8 SMEs and 1 large company.
The project GRAIL has been build with 15 partners from 9 different countries with the aim of finalising the solutions given previously to the valorization of glycerol and transform then in valuable products in a biorefinery approach. The overall concept of GRAIL project is the use, exploitation and further development of the state of the art in the field of bio-based products from glycerol and the development research-driven cluster for the use of crude glycerol for the production of high-value platforms, as well as valued end products, harnessing the biotech processes. Therefore GRAIL project has a strong business focus and its ultimate goal is to set up implantation of biorefineries in close relationship with biodiesel. This project's aim is to develop a set of technologies for converting waste glycerol from biodiesel production in a biorefinery concept to end with products of high value such as 1,3 propanediol, Fatty acid glycerol formal esters, PolyHydroxyAlkanoates (PHA), Hydrogen and Ethanol, Synthetic coatings, powder coating resins, Secondary Glycerol Amine, Biobutanol, Trehalose, Cyanocobalamin (Vitamin B12), ß-carotene, Docosahexaenoic acid (DHA), .The GRAIL project has designed an overall strategy based on three main pillars covering all the value chain: Pillar 1: Raw materials: Evaluation of crude glycerol and purification - Pillar 2: Product development: Research and development to transform crude glycerol into other high added value such as biofuels, green chemicals and food supplements - Pillar 3: Industrial feasibility aspects including economic and environmental evaluation. This pillar will take the results of GRAIL from the product development to the industrial site. To carry out that the technical feasibility will be study on a pilot plant in a Demonstration (and the results will be important to evaluate the LCA and the economic feasibility (WP6).
SYNPOL aims to propel the sustainable production of new biopolymers from feedstock. SYNPOL will theretoestablish a platform that integrates biopolymer production through modern processing technologies, withbacterial fermentation of syngas, and the pyrolysis of highly complex biowaste (e.g., municipal, commercial,sludge, agricultural). The R&D activities will focus on the integration of innovative physico-chemical, biochemical,downstream and synthetic technologies to produce a wide range of new biopolymers. The integration will engagenovel and mutually synergistic production methods as well as the assessment of the environmental benefitsand drawbacks. This integrative platform will be revolutionary in its implementation of novel microwave pyrolytictreatments together with systems-biology defined highly efficient and physiologically balanced recombinantbacteria. The latter will produce biopolymer building-blocks and polyhydroxyalkanoates that will serve tosynthesize novel bio-based plastic prototypes by chemical and enzymatic catalysis. Thus, the SYNPOL platformwill empower the treatment and recycling of complex biological and chemical wastes and raw materials in asingle integrated process. The knowledge generated through this innovative biotechnological approach will notonly benefit the environmental management of terrestrial wastes, but also reduce the harmful environmentalimpact of petrochemical plastics. This project offers a timely strategic action that will enable the EU to lead worldwide the syngas fermentation technology for waste revalorisation and sustainable biopolymer production.
Micro B3 will develop innovative bioinformatic approaches and a legal framework to make large-scale data on marine viral, bacteria; archaeal and protists genomes and metagenomes accessible for marine ecosystems biology and to define new targets for biotechnological applications. Micro B3 will build upon a highly interdisciplinary consortium of 32 academic and industrial partners comprising world-leading experts in bioinformatics, computer science, biology, ecology, oceanography, bioprospecting and biotechnology, as well as legal aspects. icro B3 is based on a strong user- and data basis from ongoing European sampling campaigns to long-term ecological research sites. For the first time a strong link between oceanographic and molecular microbial research will be established to integrate global marine data with research on microbial biodiversity and functions. The Micro B3 Information System will provide innovative open source software for data-processing, -integration, -visualisation, and -accessibility. Interoperability will be the key for seamless data transfer of sequence and contextual data to public repositories. Micro B3 will allow taking full advantage of current sequencing technologies to efficiently exploit large-scale sequence data in an environmental context. Micro B3 will create integrated knowledge to inform marine ecosystems biology and modelling. Moreover, it will facilitate detecting candidate genes to be explored by targeted laboratory experiments for biotechnology and for assigning potential functions to unknown genes. Micro B3 will develop clear IP agreements for the protection and sustainable use of pre-competitive microbial genetic resources and their exploitation in high potential commercial applications. To underline the translational character of Micro B3, outreach and training activities for diverse stakeholders are planned as well as an Ocean Sampling Day to transparently make project results accessible and gain valuable user feedback.
Das Potential der Nutzung von urbanen und landwirtschaftlichen biogenen Abfällen wird europaweit derzeit nur anteilig ausgeschöpft. FERTIPLUS zielt darauf ab, diese Potentiale im Hinblick auf die Verwertung der aus biogenen Abfällen generierten Produkte Biochar und Kompost zu bewerten. Des Weiteren wird das Projekt innovative Ansätze zur Produktion und Anwendung dieser Substrate betrachten und sich mit möglichen Risiken für die Umwelt und die Nahrungskette befassen.
The current project proposal discloses a novel biorefinery process for a sustainable, waste free, low energy conversion route of negative value marine waste streams into high value, high performance chemical intermediates and products for the polymer industry. The project has a strong emphasis on technology development and transfer to low-tech and developing countries in the EU and associated ICPC and therefore will significantly contribute to the technological and economic leadership of the EU. The technologies disclosed in this project will foster the natural growth of sustainable economies in the EU and beyond by eliminating the need for fossil resources to preserve and exceed the current standard of living. The innovative technologies developed in this project will apply novel concepts for the production of bio-based platform chemicals that act as 'drop-ins' for existing and novel polymer production processes with high atom efficiencies. The unique assembly of the current consortium consisting of academics, SME's and large scale chemical industry partners, clearly has the scientific and technical expertise to rapidly transform laboratory based results into novel product lines at an accelerated time frame. As a part of the strategy the consortium has included Demonstration Activities as require by the FP7-KBBE-Call.
PROMISE strives for multidimensional networking thus fostering integration. The primary strategic objective of PROMISE is to improve and increase the integration, collaboration and knowledge transfer between the new member states, old member states (EU15) and candidate countries through a collaborative workplan of exchange of expertise and regional training and dissemination actions, to tackle common food safety threats. PROMISE strives for sustainability through involvement of risk communicators. A further strategic objective is to integrate stakeholders like public health authorities and national food safety authorities from the old and new member countries in order to ensure the exploitation of research results into standardisation and harmonisation efforts. PROMISE will enhance the knowledge on pathogen transmission. While legal imports are well monitored for contamination and alerts are registered through the Rapid Alert System for Food and Feed RASFF notification systems, gates into the EU-27 could exist where food supply chains are not controllled. These uncontrolled imports present the risk that new strains of traditional pathogens will be transferred from third countries into the European Union. Analysing, assessing and interpreting this risk of introducing new strains of pathogens is one of the main objectives of PROMISE.
The provision of public goods (including landscape services) in rural areas is recognized as one of the key topics for the future of agriculture and rural policy. Agriculture plays a major role in landscape management through its complex interlinkages with landscape features. In turn, the Common Agricultural Policy (CAP) remains an important driver of landscape management due to its importance as a determinant of farming activities in the EU. The main objective of the CLAIM project is to provide the knowledge base to support an effective CAP policy design in the direction of improved landscape management, particularly providing insights into the ability of landscape to contribute to the production of added value for society in rural areas. CLAIM is focused in particular on understanding and enhancing the contribution of landscapes management to socio-economic development and agricultural competitiveness in rural areas. This will be based on a pragmatic consideration of landscape services and their analysis through a mixed-method approach, taking into account the wider EU policy strategies (in particular related to innovation and the bioeconomy). The main expected result of the CLAIM project is an evidence-based policy support framework on the different and possible contributions of agriculture and the CAP to landscape management. The framework will be mainly developed and validated through a set of 9 case studies, a strong involvement of stakeholders at different territorial levels and a wide coverage of the perspectives of EU and candidate countries. The framework will finally take the practical form of a web-based manual to be implemented in accordance to stakeholders needs and indications.
The BEE DOC comprises a network of eleven partners from honeybee pathology, chemistry, genetics and apicultural extension aiming to improve colony helath of honeybees. The BEE DOC will empirically and experimentally fill knowledge gaps in honey bee pest and diseases, including the 'colony collapse disorder' and quantify the impact of ineractions between parasites, pathogens and pesticides on honey bee mortality. Specifically BEE DOC will show for two model parasites (Nosema and Varroa mites), three model viruses (Deformed Wing Virus, Black Queen Cell Virus, Israel Acute Paralysis Virus) and two model pestcides (fipronil, A-fluvalinate) how interactions affect individual bees and colonies in different European areas. The BEE DOC will use transcriptome anayses to explore host-pathogen-pesticide interaction and identify novel genes for disease resistance. The BEE DOC will specifically address sublethal and chronic exposure to pesticides and screen how apicultural practices affect colony health. The BEE DOC will develop novel diagnostic screening methods and develop sustainable concepts for disease prevention using novel treatments and selection tools for resitant stock. The BEE DOC will be linked to various national and international ongoing European, North-, and Latin-American colony health monitoring and research programs, which will not only ensure a pan European but also a global visibility and the transfer of results to a world wide community of beekeepers.
SUNBIOPATH - towards a better sunlight to biomass conversion efficiency in microalgae - is an integrated program of research aimed at improving biomass yields and valorisation of biomass for two Chlorophycean photosynthetic microalgae, Chlamydomonas reinhardtii and Dunaliella salina. Biomass yields will be improved at the level of primary processes that occur in the chloroplasts (photochemistry and sunlight capture by the light harvesting complexes) and in the cell (biochemical pathways and signalling mechanisms that influence ATP synthesis). Optimal growth of the engineered microalgae will be determined in photobioreactors, and biomass yields will be tested using a scale up approach in photobioreactors of different sizes (up to 250 L), some of which being designed and built during SUNBIOPATH. Biomethane production will be evaluated. Compared to other biofuels, biomethane is attractive because the yield of biomass to fuel conversion is higher. Valorisation of biomass will also be achieved through the production of biologicals. Significant progress has been made in the development of chloroplast genetic engineering in microalgae such as Chlamydomonas, however the commercial exploitation of this technology still requires additional research. SUNBIOPATH will address the problem of maximising transgenic expression in the chloroplast and will develop a robust system for chloroplast metabolic engineering by developing methodologies such as inducible expression and trans-operon expression. A techno economic analysis will be made to evaluate the feasibility of using these algae for the purposes proposed (biologicals production in the chloroplast and/or biomethane production) taking into account their role in CO2 mitigation.
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