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Physical oceanography during RV HEINCKE cruise HE654/1

Conductivity-temperature-depth profiles were measured using a Seabird SBE 911plus CTD during RV HEINCKE cruise HE654/1. The CTD was equipped with duplicate sensors for temperature (SBE3plus), conductivity (SBE4) and oxygen (SBE43). Additional sensors such as a WET Labs C-Star transmissometer, a WET Labs ECO-AFL fluorometer and an altimeter (PSA-916 Teledyne (Benthos)) were mounted to the CTD. Temperature, conductivity and oxygen sensors are calibrated by the manufacturer once a year before being mounted in January. They are used throughout the year and no post-cruise or in-situ calibration is applied. All other sensors are calibrated irregularly. Data were connected to the station book of the specific cruise as available in the DSHIP database. Processing of the data including removal of obvious outliers followed the procedures described in CTD Processing Logbook of RV HEINCKE (hdl:10013/epic.47427). The processing report for this dataset is linked below.

Physical oceanography during RV HEINCKE cruise HE641

Conductivity-temperature-depth profiles were measured using a Seabird SBE 911plus CTD during RV HEINCKE cruise HE641. The CTD was equipped with duplicate sensors for temperature (SBE3plus), conductivity (SBE4) and oxygen (SBE43). Additional sensors such as a WET Labs C-Star transmissometer, a WET Labs ECO-AFL fluorometer and an altimeter (PSA-916 Teledyne (Benthos)) were mounted to the CTD. Temperature, conductivity and oxygen sensors are calibrated by the manufacturer once a year before being mounted in January. They are used throughout the year and no post-cruise or in-situ calibration is applied. All other sensors are calibrated irregularly. Data were connected to the station book of the specific cruise as available in the DSHIP database. Processing of the data including removal of obvious outliers followed the procedures described in CTD Processing Logbook of RV HEINCKE (hdl:10013/epic.47427). The processing report for this dataset is linked below.

Physical oceanography during RV HEINCKE cruise HE609

Conductivity-temperature-depth profiles were measured using a Seabird SBE 911plus CTD during RV Heincke cruise HE609. The CTD was equipped with duplicate sensors for temperature (SBE3plus), conductivity (SBE4) and oxygen (SBE43). Additional sensors such as a WET Labs C-Star transmissometer, a WET Labs ECO-AFL fluorometer and an altimeter (PSA-916 Teledyne (Benthos)) were mounted to the CTD. Temperature, conductivity and oxygen sensors are calibrated by the manufacturer once a year before being mounted in January. They are used throughout the year and no post-cruise or in-situ calibration is applied. All other sensors are calibrated irregularly. Data were connected to the station book of the specific cruise as available in the DSHIP database. Processing of the data including removal of obvious outliers followed the procedures described in CTD Processing Logbook of RV Heincke (hdl:10013/epic.47427). The processing report for this dataset is linked below.

Physical oceanography during RV HEINCKE cruise HE607

Conductivity-temperature-depth profiles were measured using a Seabird SBE 911plus CTD during RV Heincke cruise HE607. The CTD was equipped with duplicate sensors for temperature (SBE3plus), conductivity (SBE4) and oxygen (SBE43). Additional sensors such as a WET Labs C-Star transmissometer, a WET Labs ECO-AFL fluorometer and an altimeter (PSA-916 Teledyne (Benthos)) were mounted to the CTD. Temperature, conductivity and oxygen sensors are calibrated by the manufacturer once a year before being mounted in January. They are used throughout the year and no post-cruise or in-situ calibration is applied. All other sensors are calibrated irregularly. Data were connected to the station book of the specific cruise as available in the DSHIP database. Processing of the data including removal of obvious outliers followed the procedures described in CTD Processing Logbook of RV Heincke (hdl:10013/epic.47427). The processing report for this dataset is linked below.

Physical oceanography during RV HEINCKE cruise HE614

Conductivity-temperature-depth profiles were measured using a Seabird SBE 911plus CTD during RV HEINCKE cruise HE614. The CTD was equipped with duplicate sensors for temperature (SBE3plus), conductivity (SBE4) and oxygen (SBE43). Additional sensors such as a WET Labs C-Star transmissometer, a WET Labs ECO-AFL fluorometer and an altimeter (PSA-916 Teledyne (Benthos)) were mounted to the CTD. Temperature, conductivity and oxygen sensors are calibrated by the manufacturer once a year before being mounted in January. They are used throughout the year and no post-cruise or in-situ calibration is applied. All other sensors are calibrated irregularly. Data were connected to the station book of the specific cruise as available in the DSHIP database. Processing of the data including removal of obvious outliers followed the procedures described in CTD Processing Logbook of RV HEINCKE (hdl:10013/epic.47427). The processing report for this dataset is linked below.

Seawater carbonate chemistry and skeletal integrity of a marine keystone predator (Asterias rubens)

The current increase in atmospheric CO2 concentration induces changes in the seawater carbonate system resulting in decreased pH and calcium carbonate saturation state, a phenomenon called ocean acidification (OA). OA has long been considered as a major threat to echinoderms because their extensive endoskeleton is made of high‑magnesium calcite, one of the most soluble forms of calcium carbonate. Numerous studies addressed this question in sea urchins, but very few questioned the impact of OA on the sea star skeleton, although members of this taxon do not compensate their extracellular pH, contrary to most sea urchins. In the present study, adults of the common sea star, Asterias rubens from Kiel Fjord, a site experiencing natural acidification events exceeding pCO2 levels of 2500 μatm, were chronically exposed to different levels of simulated ocean acidification (pHT-SW 8.0, 7.4, 7.2), encompassing present and future conditions, for the duration of 109 days. Corrosion and mechanical properties of skeletal elements were studied using scanning electron microscopy, three-point bending tests as well as nanoindentation. The spines were significantly corroded at pHT-SW 7.4 and below while the ambulacral plates were only affected at pHT-SW 7.2. Nanoindentation of newly formed spines and ambulacral plates did not reveal significant CO2-induced differences in skeleton hardness or elasticity across treatments. Results of three-point bending tests revealed significantly reduced characteristic strength and fracture force of ambulacral plates from the median arm segment at pHT-SW 7.4 and below. These plates are those supporting the tube feet involved in the opening of bivalves during feeding and in the animal attachment to the substrate. Under reduced seawater pH, this might result in fracture of sea star plates during predation on mussel. The present results predict a possible impact of ocean acidification on the skeletal integrity of a marine keystone predator.

Seawater carbonate chemistry and benthic foraminifera Ammonia sp. uranium incorporation during experiments, 2013

The chemical and isotopic composition of foraminiferal shells (so-called proxies) reflects the physico-chemical properties of the seawater. In current day paleoclimate research, the reconstruction of past seawater carbonate system to infer atmospheric CO2 concentrations is one of the most pressing challenges and a variety of proxies have been investigated, such as foraminiferal U/Ca. Since in natural seawater and traditional CO2 perturbation experiments, the carbonate system parameters co-vary, it is not possible to determine the parameter of the carbonate system causing e.g. changes in U/Ca, complicating the use of the latter as a carbonate system proxy. We overcome this problem, by culturing the benthic foraminifer Ammonia sp. at a range of carbonate chemistry manipulation treatments. Shell U/Ca values were determined to test sensitivity of U incorporation to various parameters of the carbonate system. We argue that CO3 is the parameter affecting the U/Ca ratio and consequently, the partitioning coefficient for U in Ammonia sp DU. We can confirm the strong potential of foraminiferal U/Ca as a CO3 proxy.

Impact of ocean acidification on thermal tolerance and acid-base regulation of Mytilus edulis (L.) from the North Sea

Raw data of physical oceanography during RV HEINCKE cruise HE660

Raw physical oceanography data was acquired by a ship-based Seabird SBE911plus CTD-Rosette system onboard RV HEINCKE . The CTD was equipped with duplicate sensors for temperature (SBE3plus) and conductivity (SBE4) as well as one sensor for oxygen (SBE43). Additional sensors such as a WET Labs C-Star transmissometer, a WET Labs ECO-AFL fluorometer (FLRTD) and an altimeter (Teledyne Benthos PSA-916) were mounted to the CTD. The data was recorded using pre-cruise calibration coefficients. No correction, post-cruise calibration or quality control was applied. Processed profile data are available via the link below.

Raw data of physical oceanography during RV HEINCKE cruise HE654/1

Raw physical oceanography data was acquired by a ship-based Seabird SBE911plus CTD-Rosette system onboard RV HEINCKE . The CTD was equipped with duplicate sensors for temperature (SBE3plus) and conductivity (SBE4) as well as one sensor for oxygen (SBE43). Additional sensors such as a WET Labs C-Star transmissometer, a WET Labs ECO-AFL fluorometer (FLRTD) and an altimeter (Teledyne Benthos PSA-916) were mounted to the CTD. The data was recorded using pre-cruise calibration coefficients. No correction, post-cruise calibration or quality control was applied. Processed profile data are available via the link below.

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