Durum wheat is mainly grown as a summer crop. An introduction of a winter form failed until now due to the difficulty to combine winter hardiness with required process quality. Winter hardiness is a complex trait, but in most regions the frost tolerance is decisive. Thereby a major QTL, which was found in T. monococcum, T.aestivum, H. vulgare and S.cereale on chromosome 5, seems especially important. With genotyping by sequencing it is now possible to make association mapping based on very high dense marker maps, which delivers new possibilities to detect main and epistatic effects. Furthermore, new sequencing techniques allow candidate gene based association mapping. The main aim of the project is to unravel the genetic architecture of frost tolerance and quality traits in durum. Thereby, the objectives are to (1) determine the genetic variance, heritability and correlations among frost tolerance and quality traits, (2) examine linkage disequilibrium and population structure, (3) investigate sequence polymorphism at candidate genes for frost tolerance, and (4) perform candidate gene based and genome wide association mapping.
Cydia pomonella granulovirus (CpGV, Baculoviridae) is one of the most important agents for the control of codling moth (CM, Cydia pomonella, L.) in both biological and integrated pest management. The rapid emergence of resistance against CpGV-M, which was observed in about 40 European CM field populations from 2003 on, could be traced back to a single, dominant, sex-linked gene. Since then, resistance management has been based on mixtures of new CpGV isolates (CpGV-I12, -S), which are able to overcome this resistance. Recently, resistance even to these novel isolates was observed in CM field populations. This resistance does not follow the described dominant, sex-linked inheritance trait. At the same time, another isolate CpGV-V15 was identified showing high virulence against these resistant populations. To elucidate this novel resistance mechanism and to identify the resistance gene(s) involved, we propose a comprehensive analysis of this resistance on the cellular and genomic level of codling moth. Because of the lack of previous knowledge of the molecular mechanisms of virus resistance in insects, several different and complementary approaches will be pursued. This study will not only give an in-depth insight into the genetic possibilities for development of baculovirus resistance in CM field populations and how the virus overcomes it, but can also serve as an important model for other baculovirus-host interaction systems.
Ziel des Projektes ist eine Bestandsaufnahme der Wassermassenverteilung und der Zirkulation im Arktischen Ozean. Stabile Sauerstoffisotopen (delta18O) des Wassers ist ein konservativer Tracer und werden zusammen mit hydrochemischen Daten dazu verwendet das vom Schelf stammende Süßwasser (Flusswasser und Meereis-Schmelze oder Bildung) und die aus dem Pazifik stammende Komponente zu untersuchen. Auf diese Weise wird der Einfluss dieser Wassermassen in der arktischen Salzgehaltsschichtung (Halokline), dem Atlantischen Zwischenwasser und dem Tiefen- und Bodenwasser des Arktischen Ozeans quantifiziert werden. Es ist bekannt, dass die Verteilung der Pazifischen Komponente starken Veränderungen auf dekadischen Zeitskalen unterliegt aber auch in den Süßwasserverteilungen im Transpolaren Drift Strom wurden 2007 starke Variationen beobachtet welche somit auf zusätzliche jährliche Variationen hinweisen. Es ist nicht bekannt ob die 2007 beobachteten Variationen ein permanentes Phänomen sind und ob diese mit dem weitgehenden Fehlen des Pazifischen Wassers in diesem Zeitraum zusammenhängen. Die geplante flächendeckende und quantitative Erfassung der Süßwasserverteilung und des Pazifischen Wassers werden daher dazu beitragen, den Einfluss und die möglichen Rückkopplungsmechanismen der arktischen Hydrographie auf den arktischen und globalen Klimawandel weitergehend zu verstehen.
This subproject aims to analyze the fragmentation of forest policy at both an international and national level for the selected countries, employing a discourse analysis approach. It is split into two sub-subprojects (SSPs). 'SSPa' conducts an analysis of discursive genealogies of forest policy in Germany, Sweden, and the US. 'SSPb' investigates the history of forest related discourses in three global environmental policy processes (UNFF, CBD, and UNFCCC). In doing so, both SSPs follow a three step procedure: In the first work package, relevant literature is reviewed and a theoretical and analytical framework is developed. In the second work package, empirical data (mostly formal and informal policy documents) are gathered and analyzed. In the third work package, emphasis is placed on the role of political 'elites' in the creation of fragmented forest policy discourses at different levels; in-depth interviews with policy stakeholders and experts add another perspective to the analysis in this work package. The project is expected to develop a new understanding not only of the fragmentation of multi-level and multi-sector forest policy discourses, but also of the way in which 'discourse elites' interact with and within these discourses. The results of the work packages will be published in peer reviewed journals and discussed with policy stakeholders and scientists in conferences and workshops.
In recent years science has taken an increased interest in mineralization processes in tropical soils in particular under minimal tillage operations. Plant litter quality and management strongly affect mineralization-nitrification processes in soil and hence the fate of nitrogen in ecosystems and the environment. Plant secondary metabolites like lignin and polyphenols are poorly degradable and interact with proteins (protein binding capacity) and hence protect them from microbial attack. Nitrification, a microbiological process, directly and indirectly influences the efficiency of recovery of N in the vegetation as well as the loss of N (through denitrification and leaching) causing environmental pollution to water bodies and contributes to global warming (e.g. the greenhouse gas N2O is emitted as a by-product of nitrification and denitrification). Nitrifiers comprise a relatively narrow species diversity (at least as known to date) and are generally thought to be sensitive to low soil pH and stress. Despite these properties nitrification occurs in acid tropical soils with high levels of aluminium and manganese. Thus the main objective of the project will be the identification of micro-organisms and mechanisms responsible for mineralization-nitrification processes in acid tropical soils and the influence of long-term litter input of different chemical qualities and minimal tillage options. The project will include the use of stable isotopes (15N, 13C), mass spectrometry, gas chromatography (CO2, N2O), biochemical methods (PLFA) and molecular biology (16s rRNA., PCR, DGGE)
Die ständige Weiterentwicklung und Verbesserung der Wetter- und Klimamodelle stellt die Fernerkundung der Atmosphäre vor große Herausforderungen. Für die Evaluierung der Modelle werden immer besser aufgelöste Messungen und Methoden benötigt. Herkömmliche Ansätze scheitern hier vor allem an fehlenden kontinuierlichen Beobachtungen der Temperatur und Feuchte bei allen Wetterbedingungen und insbesondere bei Regen. Ein Windprofiler ist allerdings auch bei solchen Bedingungen in der Lage Vertikalinformationen der Temperatur- und Feuchtegradienten zu messen. Der hier vorgeschlagene neuartige Ansatz aus einer Synergie aus Windprofiler (inklusive Radio Acoustic Sounding System), Ramanlidar, Mikrowellenradiometer und Wolkenradar ermöglicht eine automatisierte und kontinuierliche Erstellung von Temperatur- und Feuchteprofilen sogar bei Niederschlägen. Die zu verwendende variationelle Methode (optimale Schätzung, in engl. â€Ìoptimal estimationâ€Ì) bietet dabei ein robustes Hilfsmittel für die Kombination mehrerer Messgeräte unter Einbeziehung der Unsicherheiten der einzelnen Systeme. Bei der optimalen Schätzung wird ein vorgegebener Anfangszustand (z.B. die Klimatologie des Standorts oder der letzte bekannte Zustand) so lange iterativ variiert, bis er mit den Beobachtungen der verschiedenen Messgeräte innerhalb der Unsicherheiten übereinstimmt. Die Methode ermöglicht auch eine ausführliche Analyse der Unsicherheiten der Resultate und eine Einschätzung der Beiträge der einzelnen Geräte.Die langen Zeitreihen an Daten und die Kombination an sich ergänzenden Messinstrumenten, insbesondere mit dem 482 MHz Windprofiler am Meteorologischen Observatorium Lindenberg â€Ì Richard Aßmann Observatorium (MOL-RAO), sind einzigartig. Der Antragsteller kann hier seine umfangreichen Erfahrungen mit Instrumentensynergie und der Entwicklung von Algorithmen zur Ableitung atmosphärischer Variablen einbringen, um eine kontinuierliche Zeitreihe von Temperatur- und Feuchteprofilen mit bisher nicht erreichter Genauigkeit innerhalb und oberhalb von Wolken und insbesondere bei Niederschlag zu erstellen. Die thermodynamischen Profile bieten die ideale Möglichkeit, die Verdunstungsraten und die daraus resultierende Abkühlung mit einer verbesserten Genauigkeit zu quantifizieren. Die Unsicherheiten, die durch ungenaue Profile der relativen Feuchte und Temperatur entstehen, werden mit Hilfe von Simulationen abgeschätzt. Langzeitbeobachtungen an MOL-RAO werden genutzt, um aussagekräfige Statistiken über die Verdunstungs- und Abkühlungsraten zu erstellen. Die Ergebnisse werden für verschiedene Bedingungen wie stratiformen und konvektiven Niederschlag und für verschiedenen Jahreszeiten evaluiert. Dies wird den Modellieren helfen, die Parametrisierungen der Verdunstungsraten in kleinskaligen Modellen zu evaluieren.
It is well established that reduced supply of fresh organic matter, interactions of organic matter with mineral phases and spatial inaccessibility affect C stocks in subsoils. However, quantitative information required for a better understanding of the contribution of each of the different processes to C sequestration in subsoils and for improvements of subsoil C models is scarce. The same is true for the main controlling factors of the decomposition rates of soil organic matter in subsoils. Moreover, information on spatial variabilities of different properties in the subsoil is rare. The few studies available which couple near and middle infrared spectroscopy (NIRS/MIRS) with geostatistical approaches indicate a potential for the creation of spatial maps which may show hot spots with increased biological activities in the soil profile and their effects on the distribution of C contents. Objectives are (i) to determine the mean residence time of subsoil C in different fractions by applying fractionation procedures in combination with 14C measurements; (ii) to study the effects of water content, input of 13C-labelled roots and dissolved organic matter and spatial inaccessibility on C turnover in an automatic microcosm system; (iii) to determine general soil properties and soil biological and chemical characteristics using NIRS and MIRS, and (iv) to extrapolate the measured and estimated soil properties to the vertical profiles by using different spatial interpolation techniques. For the NIRS/MIRS applications, sample pretreatment (air-dried vs. freeze-dried samples) and calibration procedures (a modified partial least square (MPLS) approach vs. a genetic algorithm coupled with MPLS or PLS) will be optimized. We hypothesize that the combined application of chemical fractionation in combination with 14C measurements and the results of the incubation experiments will give the pool sizes of passive, intermediate, labile and very labile C and N and the mean residence times of labile and very labile C and N. These results will make it possible to initialize the new quantitative model to be developed by subproject PC. Additionally, we hypothesize that the sample pretreatment 'freeze-drying' will be more useful for the estimation of soil biological characteristics than air-drying. The GA-MPLS and GA-PLS approaches are expected to give better estimates of the soil characteristics than the MPLS and PLS approaches. The spatial maps for the different subsoil characteristics in combination with the spatial maps of temperature and water contents will presumably enable us to explain the spatial heterogeneity of C contents.
Boron (B) is an essential microelement for plants. Despite the use of modern fertilization methods, B deficiency still causes losses in agricultural plant production. Even though many positive effects of B on plant growth and physiology have been reported, a large majority of B functions and the regulatory mechanisms controlling the B nutritional status remain unknown. The main objective of this project is to elucidate how the greatly B deficiency-sensitive Brassica crop plants process and regulate their B status during vegetative and reproductive growth. In this context, the project aims at identifying the mode of action of B in mechanisms regulating the B status itself and uncovering those mechanisms contributing to B efficiency in different genotypes. Plant species subjected to investigation will be the agronomically important oilseed and vegetable plant Brassica napus (rapeseed) and its close relative the genetic and molecular model plant Arabidopsis thaliana. Questions addressed within the scope of this project should lead to a detailed understanding of mechanisms controlling B uptake and allocation from the level of the whole plant down to the cellular level. B transport routes and rates will be determined in sink- and source tissues and in developmental periods with a particularly high B demand. A special focus will be on the identification of B transport bottlenecks and the analysis of B deficiency-sensitive transport processes to and within the highly B-demanding reproductive organs. Recent studies in Arabidopsis suggest that Nodulin26-like Intrinsic Proteins (NIPs), which belong to the aquaporin channel protein family, are essential for plant B uptake and distribution. The systematic focus on the molecular and physiological characterization of B. napus NIPs will clarify their role in B transport and will identify novel NIP-associated mechanisms playing key roles in the B response network.To further resolve the mostly unknown impact of the B nutritional status on gene regulation and metabolism, a transcript and metabolite profile of B-sufficient and B-deficient rapeseed plants will be generated. Additionally, an Arabidopsis transcription factor knockout collection (greater 300 lines) will be screened for abnormalities in responses to the B nutritional status. This will identify yet unknown B-responsive genes (transcription factors and their targets) and gene products (enzymes or metabolite variations) playing key roles in signalling pathways and mechanisms regulating the B homeostasis. Boron (in form of boric acid) and arsenite (As) share in all likelihood the same NIP-mediated transport pathways. To assess the consequences of this dual transport pathway the so far unstudied impact of the plants B nutritional status on the accumulation and distribution of As will be investigated in B. napus. Moreover, the current dimension of the As contamination of Brassica-based food products, to which consumers are exposed to, will be analyzed. usw.
In diesem Vorhaben wird die Wechselwirkung von Photosynthesepigmenten und Proteinen auf molekularer Ebene untersucht, um besser zu verstehen, unter welchen Bedingungen absorbiertes Licht für die Photosynthese genutzt wird und unter welchen Bedingungen die Energie als Wärmeabstrahlung verloren geht. Ein genaueres Verständnis der zugrunde liegenden Funktionsmechanismen ist z.B. notwendig, um in Zukunft pflanzliche Carotinoide verstärkt in der Biotechnologie einsetzen zu können. In dem Vorhaben wird die molekulare Pigmentorganisation und ihre lichtabhängige Dynamik in einer einzelligen Alge untersucht, die sich durch eine besonders reichhaltige Pigmentierung auszeichnet. Es konnte gezeigt werden, dass der dem Lichtschutz dienende Xanthophyllzyklus bei Diatomeen zusätzlich eine zentrale Rolle in der Regulation der Pigmentbiosynthese spielt. In Mantoniella konnte ein unvollständiger Xanthopyllzyklus nachgewiesen werden. Damit eröffnet sich die Möglichkeit, die Evolution des Lichtschutzes in der Photosynthese experimentell zu untersuchen.
In subsoils, organic matter (SOM) concentrations and microbial densities are much lower than in topsoils and most likely highly heterogeneously distributed. We therefore hypothesize, that the spatial separation between consumers (microorganisms) and their substrates (SOM) is an important limiting factor for carbon turnover in subsoils. Further, we expect microbial activity to occur mainly in few hot spots, such as the rhizosphere or flow paths where fresh substrate inputs are rapidly mineralized. In a first step, the spatial distribution of enzyme and microbial activities in top- and subsoils will be determined in order to identify hot spots and relate this to apparent 14C age, SOM composition, microbial community composition and soil properties, as determined by the other projects within the research unit. In a further step it will be determined, if microbial activity and SOM turnover is limited by substrate availability in spatially distinct soil microsites. By relating this data to root distribution and preferential flow paths we will contribute to the understanding of stabilizing and destabilizing processes of subsoil organic matter. As it is unclear, at which spatial scale these differentiating processes are effective, the analysis of spatial variability will cover the dm to the mm scale. As spatial segregation between consumers and substrates will depend on the pore and aggregate architecture of the soil, the role of the physical integrity of these structures on SOM turnover will also be investigated in laboratory experiments.
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