Existing models of soil organic matter (SOM) formation consider plant material as the main source of SOM. Recent results from nuclear magnetic resonance analyses of SOM and from own incubation studies, however, show that microbial residues also contribute to a large extent to SOM formation. Scanning electron microscopy showed that the soil mineral sur-faces are covered by numerous small patchy fragments (100 - 500 nm) deriving from microbial cell wall residues. We will study the formation and fate of these patchy fragments as continuously produced interfaces in artificial soil systems (quartz, montmorillonite, iron oxides, bacteria and carbon sources). We will quantify the relative contributions of different types of soil organisms to patchy fragment formation and elucidate the effect of redox con-ditions and iron mineralogy on the formation and turnover of patchy fragments. The develop-ment of patchy fragments during pedogenesis will be followed by studying soil samples from a chronosequence in the forefield of the retreating Damma glacier. We will characterize chemical and physical properties of the patchy fragments by nanothermal analysis and microscale condensation experiments in an environmental scanning electron microscope. The results will help understanding the processes at and characteristics of biogeochemical interfaces.
Methane (CH4) is a major greenhouse gas of which the atmospheric concentration has more than doubled since pre-industrial times. Soils can act as both, source and sink for atmospheric CH4, while upland forest soils generally act as CH4 consumers. Oxidation rates depend on factors influenced by the climate like soil temperature and soil moisture but also on soil properties like soil structure, texture and chemical properties. Many of these parameters directly influence soil aeration. CH4 oxidation in soils seems to be controlled by the supply with atmospheric CH4, and thus soil aeration is a key factor. We aim to investigate the importance of soil-gas transport-processes for CH4 oxidation in forest soils from the variability the intra-site level, down to small-scale (0.1 m), using new approaches of field measurements. Further we will investigate the temporal evolution of soil CH4 consumption and the influence of environmental factors during the season. Based on previous results, we hypothesize that turbulence-driven pressure-pumping modifies the transport of CH4 into the soil, and thus, also CH4 consumption. To improve the understanding of horizontal patterns of CH4 oxidation we want to integrate the vertical dimension on the different scales using an enhanced gradient flux method. To overcome the constraints of the classical gradient method we will apply gas-diffusivity measurements in-situ using tracer gases and Finite-Element-Modeling. Similar to the geophysical technique of Electrical Resistivity Tomography we want to develop a Gas Diffusivity Tomography. This will allow to derive the three-dimensional distribution of soil gas diffusivity and methane oxidation.
Mikroorganismen sind im Boden, in kryptogamen Gemeinschaften und in der Atmosphäre von zentraler Bedeutung. Verschiedene Spezies von Bakterien, Pilzen, Flechten und Pollen wurden bereits als Eiskeime, welche eine Eisbildung bei relativ hohen Temperaturen initiieren können, identifiziert, und besonders biologische Bestandteile aus dem Boden sind eine vermutlich bedeutsame Quelle atmosphärischer Eiskeime. Die genauen Quellen biologischer Eiskeime in der Atmosphäre sind jedoch kaum bekannt, obwohl ein potentieller Beitrag dieser, zur Eis- und Niederschlagsbildung mittlerweile von verschiedenen Studien untermauert wird. Aktuelle Untersuchungen verschiedener Boden- und Luftproben zeigen Hinweise, dass verschiedene eisaktive Pilze unterschiedlicher Phyla nicht nur im Boden und in der Luft vorhanden sind, sondern auch häufig in der kultivierbaren Fraktion vorkommen können. Aus diesem Grund befasst sich das vorgeschlagene Projekt mit der Suche nach weiteren bisher unbekannten eisaktiven Mikroorganismen und Bestandteilen aus dem Boden, von Pflanzen und kryptogamen Gemeinschaften und mit der Erforschung ihres Einflusses auf die Eiskeimaktivität des Bodens. Die nötigen Methoden für ein Screening verschiedenster Kulturen z.B. von Cyanobakterien sind in unserem Labor gut etabliert. Zudem sollen die jeweiligen Eiskeime der neu gefundenen eisaktiven Organismen auf molekularer Ebene charakterisiert werden.
In diesem Vorhaben wird die Wechselwirkung von Photosynthesepigmenten und Proteinen auf molekularer Ebene untersucht, um besser zu verstehen, unter welchen Bedingungen absorbiertes Licht für die Photosynthese genutzt wird und unter welchen Bedingungen die Energie als Wärmeabstrahlung verloren geht. Ein genaueres Verständnis der zugrunde liegenden Funktionsmechanismen ist z.B. notwendig, um in Zukunft pflanzliche Carotinoide verstärkt in der Biotechnologie einsetzen zu können. In dem Vorhaben wird die molekulare Pigmentorganisation und ihre lichtabhängige Dynamik in einer einzelligen Alge untersucht, die sich durch eine besonders reichhaltige Pigmentierung auszeichnet. Es konnte gezeigt werden, dass der dem Lichtschutz dienende Xanthophyllzyklus bei Diatomeen zusätzlich eine zentrale Rolle in der Regulation der Pigmentbiosynthese spielt. In Mantoniella konnte ein unvollständiger Xanthopyllzyklus nachgewiesen werden. Damit eröffnet sich die Möglichkeit, die Evolution des Lichtschutzes in der Photosynthese experimentell zu untersuchen.
In subsoils, organic matter (SOM) concentrations and microbial densities are much lower than in topsoils and most likely highly heterogeneously distributed. We therefore hypothesize, that the spatial separation between consumers (microorganisms) and their substrates (SOM) is an important limiting factor for carbon turnover in subsoils. Further, we expect microbial activity to occur mainly in few hot spots, such as the rhizosphere or flow paths where fresh substrate inputs are rapidly mineralized. In a first step, the spatial distribution of enzyme and microbial activities in top- and subsoils will be determined in order to identify hot spots and relate this to apparent 14C age, SOM composition, microbial community composition and soil properties, as determined by the other projects within the research unit. In a further step it will be determined, if microbial activity and SOM turnover is limited by substrate availability in spatially distinct soil microsites. By relating this data to root distribution and preferential flow paths we will contribute to the understanding of stabilizing and destabilizing processes of subsoil organic matter. As it is unclear, at which spatial scale these differentiating processes are effective, the analysis of spatial variability will cover the dm to the mm scale. As spatial segregation between consumers and substrates will depend on the pore and aggregate architecture of the soil, the role of the physical integrity of these structures on SOM turnover will also be investigated in laboratory experiments.
Electrical conductivity is a key parameter in models of magnetic field generation in planetary interiors through magneto-hydrodynamic convection. Measurements of this key material parameter of liquid metals is not possible to date by experiments at relevant conditions, and dynamo models rely on extrapolations from low pressure/temperature experiments, or more recently on ab-initio calculations combining molecular dynamics and linear response calculations, using the Kubo-Greenwood formulation of transport coefficients. Such calculations have been performed for Fe, Fe-alloys, H, He and H-He mixtures to cover the interior of terrestrial and giant gas planets. These simulations are computationally expensive, and an efficient accurate scheme to determine electrical conductivities is desirable. Here we propose a model that can, at much lower computational costs, provide this information. It is based on Ziman theory of electrical conductivity that uses information on the liquid structure, combined with an internally consistent model of potentials for the electron-electron, electron-atom, and atom-atom interactions. In the proposal we formulate the theory and expand it to multi-component systems. We point out that fitting the liquid structure factor is the critical component in the process, and devise strategies on how this can be done efficiently. Fitting the structure factor in a thermodynamically consistent way and having a transferable electron-atom potential we can then relatively cheaply predict the electrical conductivity for a wide range of conditions. Only limited molecular dynamics simulations to obtain the structure factors are required.In the proposed project we will test and advance this model for liquid aluminum, a free-electron like metal, that we have studied with the Kubo-Greenwood method previously. We will then be able to predict the conductivities of Fe, Fe-light elements and H, He, as well as the H-He system that are relevant to the planetary interiors of terrestrial and giant gas planets, respectively.
Soil organic matter (SOM) controls large part of the processes occurring at biogeochemical interfaces in soil and may contribute to sequestration of organic chemicals. Our central hypothesis is that sequestration of organic chemicals is driven by physicochemical SOM matrix aging. The underlying processes are the formation and disruption of intermolecular bridges of water molecules (WAMB) and of multivalent cations (CAB) between individual SOM segments or between SOM and minerals in close interaction with hydration and dehydration mechanisms. Understanding the role of these mediated interactions will shed new light on the processes controlling functioning and dynamics of biogeochemical interfaces (BGI). We will assess mobility of SOM structural elements and sorbed organic chemicals via advanced solid state NMR techniques and desorption kinetics and combine these with 1H-NMR-Relaxometry and advanced methods of thermal analysis including DSC, TGADSC- MS and AFM-nanothermal analysis. Via controlled heating/cooling cycles, moistening/drying cycles and targeted modification of SOM, reconstruction of our model hypotheses by computational chemistry (collaboration Gerzabek) and participation at two larger joint experiments within the SPP, we will establish the relation between SOM sequestration potential, SOM structural characteristics, hydration-dehydration mechanisms, biological activity and biogechemical functioning. This will link processes operative on the molecular scale to phenomena on higher scales.
Cydia pomonella granulovirus (CpGV, Baculoviridae) is one of the most important agents for the control of codling moth (CM, Cydia pomonella, L.) in both biological and integrated pest management. The rapid emergence of resistance against CpGV-M, which was observed in about 40 European CM field populations from 2003 on, could be traced back to a single, dominant, sex-linked gene. Since then, resistance management has been based on mixtures of new CpGV isolates (CpGV-I12, -S), which are able to overcome this resistance. Recently, resistance even to these novel isolates was observed in CM field populations. This resistance does not follow the described dominant, sex-linked inheritance trait. At the same time, another isolate CpGV-V15 was identified showing high virulence against these resistant populations. To elucidate this novel resistance mechanism and to identify the resistance gene(s) involved, we propose a comprehensive analysis of this resistance on the cellular and genomic level of codling moth. Because of the lack of previous knowledge of the molecular mechanisms of virus resistance in insects, several different and complementary approaches will be pursued. This study will not only give an in-depth insight into the genetic possibilities for development of baculovirus resistance in CM field populations and how the virus overcomes it, but can also serve as an important model for other baculovirus-host interaction systems.
Durum wheat is mainly grown as a summer crop. An introduction of a winter form failed until now due to the difficulty to combine winter hardiness with required process quality. Winter hardiness is a complex trait, but in most regions the frost tolerance is decisive. Thereby a major QTL, which was found in T. monococcum, T.aestivum, H. vulgare and S.cereale on chromosome 5, seems especially important. With genotyping by sequencing it is now possible to make association mapping based on very high dense marker maps, which delivers new possibilities to detect main and epistatic effects. Furthermore, new sequencing techniques allow candidate gene based association mapping. The main aim of the project is to unravel the genetic architecture of frost tolerance and quality traits in durum. Thereby, the objectives are to (1) determine the genetic variance, heritability and correlations among frost tolerance and quality traits, (2) examine linkage disequilibrium and population structure, (3) investigate sequence polymorphism at candidate genes for frost tolerance, and (4) perform candidate gene based and genome wide association mapping.
Neue Befunde zeigen, dass die Umsetzung von organischem Material durch Bakterioneuston in marinen Oberflächenfilmen (engl. sea-surface microlayer, SML) eine Rolle spielen, bei der Kontrolle des Flusses von klimarelevanten Spurengasen, sowie bei der Speicherung von anthropogenen Ablagerungen aus der Atmosphäre. Unsere früheren Untersuchungen weisen auf die Bedeutung der bakteriellen Aktivität bzgl. des Kohlenstoffzyklus in der SML hin, heben aber auch die vielen unbekannten Einflüsse des Bakterioneuston in der SML hervor. Besonders photochemische Umwandlung von organischem Material, in Verbindung mit schädlichen Effekten durch Sonnenstrahlung auf die Bakterien in der SML, wurden bisher nicht detailliert untersucht. Demzufolge besteht unsere Motivation für das vorgeschlagene Projekt darin, das Wissen über Stoffwechselprozesse und Anpassungen von Bakterien in der SML in ihrer Gesamtheit, aber auch auf Einzelzell- und Stammebene, in Bezug auf physikalische und chemische Variabilität in dieser Grenzschicht zu untersuchen. Des Weiteren ist das Zooplankton eine wichtige Komponente für die Verbindung zwischen Phytoplankton, Bakterien und den höheren Ebenen des Nahrungsnetzes in der SML, wobei auch hier für das Verständnis relevante Daten fehlen. Daher werden wir im Rahmen des Projektes zum ersten Mal Studien zur Verbreitung des Zooplanktons sowie zu dessen Fressverhalten in der SML der Nordsee durchführen.
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