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Optimization of a Method for the Isolation of Chloroplasts from Fresh Plant Material for the Development of a Bioassay Test for the Determination of Pesticides in Water

Bio-assays are increasingly used in supplement to classical analyses to determine the effect of contamination of waters with herbicides, some of which have been shown to be able to determine herbicides within the limits in compliance with EC-ordonance for drinking water. Preliminary work carried out at the University of Bonn has demonstrated that contamination of different water systems can be identified using inhibition of the light dependent production of oxygen by chloroplasts. Further experiments at IRMM have shown a potential to transfer membrane systems of chloroplasts into stable powder that can be used to carry out such bio-assays. Results: A method has been developed tor the isolation and breakage ot chloroplasts that allow freeze drying of the thylakoid membranes. The photosynthetic activity of the lyophilized material was maintained to 86 - 95 per cent. This powder can be stored for over five month without loss of activity.

Linking internal pattern dynamics and integral responses - Identification of dominant controls with a strategic sampling design

In hydrology, the relationship between water storage and flow is still fundamental in characterizing and modeling hydrological systems. However, this simplification neglects important aspects of the variability of the hydrological system, such as stable or instable states, tipping points, connectivity, etc. and influences the predictability of hydrological systems, both for extreme events as well as long-term changes. We still lack appropriate data to develop theory linking internal pattern dynamics and integral responses and therefore to identify functionally similar hydrological areas and link this to structural features. We plan to investigate the similarities and differences of the dynamic patterns of state variables and the integral response in replicas of distinct landscape units. A strategic and systematic monitoring network is planned in this project, which contributes the essential dynamic datasets to the research group to characterize EFUs and DFUs and thus significantly improving the usual approach of subdividing the landscape into static entities such as the traditional HRUs. The planned monitoring network is unique and highly innovative in its linkage of surface and subsurface observations and its spatial and temporal resolution and the centerpiece of CAOS.

CO2 degassing from karstic springs in Southern Germany

This study examines characteristics of dissolved inorganic carbon (DIC) and partial pressures of CO2 characteristics (pCO2) in the source springs and headwaters of four karstic watersheds, via dissolved inorganic carbon concentration and stable carbon isotope measurements. All four spring sources are located in Southern Germany and were measured for water chemistry and stable isotopes with nearby headwater stream points, which were located up to 100 m downstream of the discharge points. Seasonal sampling covered winter, spring, summer, and autumn in 2018.

Results of palynological analysis from 2020 of the varved MO-05 core from Lake Mondsee (Austria) section (249-526 cm)

This study reports a precisely dated pollen record with a 20-year resolution from the varved sediments of Lake Mondsee in the north-eastern European Alps (47°49′N, 13°24′E, 481 m above sea level). The analysed part of core spans the interval between 1500 BCE and 500 CE and allows changes in vegetation composition in relation to climatic changes and human activities in the catchment to be inferred. Intervals of distinct but modest human impact are identified at ca. 1450-1220, 740-490 and 340-190 BCE and from 80 BCE to 180 CE. While the first two intervals are synchronous with prominent salt mining phases during the Bronze Age and Early Iron Age at the nearby UNESCO World Heritage Site of Hallstatt, the last two intervals fall within the Late Iron Age and Roman Imperial Era, respectively. Comparison with published records of extreme runoff events obtained from the same sediment core shows that human activities (including agriculture and logging) around Lake Mondsee were low during intervals of high flood frequency as indicated by a higher number of intercalated detrital event layers, but intensified during hydrologically stable intervals. Comparison of the pollen percentages of arboreal taxa with the stable oxygen isotope and potassium ion records of the NGRIP and GISP2 ice cores from Greenland reveals significant positive correlations for Fagus and negative correlations for Betula and Alnus. This underlines the sensitivity of vegetation around Lake Mondsee to temperature fluctuations in the North Atlantic as well as to moisture fluctuations controlled by changes in the intensity of the Siberian High and the North Atlantic Oscillation (NAO) regime.

Alkane abundance and stable isotope measurements from ELSA stack lake sediments, Eifel Volcanic Field, Germany

n-alkane peak areas from GC-FID measurements. Compound specific hydrogen and carbon isotope measurements made using GC-IRMS. Samples taken from Auel Maar, Holzmaar, and Schalkenmehrener maar lake sediment cores spanning 60,000 years. Age model information and additional proxy data from the ELSA-20 stack are found in Sirocko et al., 2021 (Nature Geoscience) and Sirocko et al., 2022 (Scientific Reports). Full methodological details are found in Zander et al., 2025 (Rapid Communications in Mass Spectrometry).

Geochemical parameters in peat depth profiles from ombrotrophic bogs in North and Central Europe. Fochteloër Veen, the Netherlands

This dataset contains geochemical variables measured in six depth profiles from ombrotrophic peatlands in North and Central Europe. Peat cores were taken during the spring and summer of 2022 from Amtsvenn (AV1), Germany; Drebbersches Moor (DM1), Germany; Fochteloër Veen (FV1), the Netherlands; Bagno Kusowo (KR1), Poland; Pichlmaier Moor (PI1), Austria and Pürgschachen Moor (PM1), Austria. The cores AV1, DM1 and KR1 were taken using a Wardenaar sampler (Royal Eijkelkamp, Giesbeek, the Netherlands) and had diameter of 10 cm. The cores FV1, PM1 and PI1 had an 8 cm diameter and were obtained using an Instorf sampler (Royal Eijkelkamp, Giesbeek, the Netherlands). The cores FV1, DM1 and KR1 were 100 cm, core AV1 was 95 cm, core PI1 was 85 cm and core PM1 was 200 cm. The cores were subsampeled in 1 cm (AV1, DM1, KR1, FV1) and 2 cm (PI1, PM1) sections. The subsamples were milled after freeze drying in a ballmill using tungen carbide accesoires. X-Ray Fluorescence (WD-XRF; ZSX Primus II, Rigaku, Tokyo, Japan) was used to determine Al (μg g-1), As (μg g-1), Ba (μg g-1), Br (μg g-1), Ca (g g-1), Cl (μg g-1), Cr (μg g-1), Cu (μg g-1), Fe (g g-1), K (g g-1), Mg (μg g-1), Mn (μg g-1), Na (μg g-1), P (μg g-1), Pb (μg g-1), Rb (μg g-1), S (μg g-1), Si (μg g-1), Sr (μg g-1), Ti (μg g-1) and Zn (μg g-1). These data were processed and calibrated using the iloekxrf package (Teickner & Knorr, 2024) in R. C, N and their stable isotopes were determined using an elemental analyser linked to an isotope ratio mass spectrometer (EA-3000, Eurovector, Pavia, Italy & Nu Horizon, Nu Instruments, Wrexham, UK). C and N were given in units g g-1 and stable isotopes were given as δ13C and δ15N for stable isotopes of C and N, respectively. Raw data C, N and stable isotope data were calibrated with certified standard and blank effects were corrected with the ilokeirms package (Teickner & Knorr, 2024). Using Fourier Transform Mid-Infrared Spectroscopy (FT-MIR) (Agilent Cary 670 FTIR spectromter, Agilent Technologies, Santa Clara, Ca, USA) humification indices (HI) were determined. Spectra were recorded from 600 cm-1 to 4000 cm-1 with a resolution of 2 cm-1 and baselines corrected with the ir package (Teickner, 2025) to estimate relative peack heights. The HI (no unit) for each sample was calculated by taking the ratio of intensities at 1630 cm-1 to the intensities at 1090 cm-1. Bulk densities (g cm-3) were estimated from FT-MIR data (Teickner et al., in preparation).

The diet contribution and habitat selection of the common periwinkle Littorina littorea depending on infection with parasites and seasonality

The common periwinkle Littorina littorea is an ecologically important grazer and serves as the first intermediate host for several trematode species in the Baltic Sea, especially for the fluke Cryptocotyle lingua. In this series of experiments and analyses, we tested whether the food sources contributing to the diet and the habitat selection differ depending on the infection status of the periwinkle and the season. (1) A spatial pattern analysis was conducted to investigate the habitat composition and availability of food sources at the study site Möltenort, Kiel Bight (54.37°N, 10.19°E), (2) the habitat choice of the periwinkle was observed in-situ by a mark and recapture experiment, and (3) the composition of the diet of L. littorea (based on stable isotope composition of carbon and nitrogen isotopes) was analysed. All experiments were conducted in spring, summer and autumn.

Environmental Determinants of Microbial Biopolymer Degraders in Agricultural Soil

The structural polysaccharides cellulose and chitin of plants, fungi, and arthropods are major components of organic matter in agricultural soils. These biopolymers are carbon sources of soil microbial communities linked to soil redox processes. Soil aggregates of waterunsaturated soil form natural boundaries of oxic conditions outside and oxygen-limited conditions inside. These biogeochemical interfaces lead to a highly heterogeneous oxygen distribution on a millimetre scale. The effects and mechanisms of the toxicity of herbicides on biopolymer degrading communities in such highly compartmentalized soils have not been resolved. The proposed study is a continuation of a project funded within Priority Program 1315 'Biogeochemical Interfaces in Soil'. The preceding project resolved phylogenetic identities of known and novel prokaryotes linked to cellulose degradation under both oxic and anoxic conditions, and demonstrated that the acidic herbicides Bentazon and MCPA impair microbial processes involved in cellulose degradation. The proposed project will (I) identify chitin-degrading prokaryotes, fungi, and protists that are active in oxic and anoxic microzones, (II) determine the tolerance of various cellulolytic and chitinolytic taxa to Bentazon and MCPA, (III) characterize key chitin-degraders, and (IV) will quantitatively assess oxygen distribution in during biopolymer degradation in an agricultural soil. Central methods will include stable isotope probing, analyses of 16S rRNA, 18S rRNA, and chitinase genes, HPLC, GC, and oxygen sensing via analysis of fluorescence dyes.

Immissions- und Strahlenschutz (GB 2)

• Überwachung der Radioaktivität in der Umwelt nach dem Strahlenschutzvorsorgegesetz für den Freistaat Sachsen • Überwachung der anlagenbezogenen Radioaktivität nach dem Atomgesetz am Forschungsstandort Rossendorf • Überwachung von Lebensmitteln (u. a. Amtshilfe für die Landesuntersuchungsanstalt für das Gesundheits- und Veterinärwesen Sachsen) • Betrieb der Radonberatungsstelle • Überwachung der anlagenbezogenen Radioaktivität nach der Verordnung zur Gewährleistung von Atomsicherheit und Strahlenschutz an den Standorten der Wismut GmbH • Überwachung der anlagenbezogenen Radioaktivität an den Altstandorten des Uranerzbergbaus • Aufsichtliche Messungen nach der Strahlenschutzverordnung inkl. Sicherheitstechnisch bedeutsame Ereignisse und Nukleare Nachsorge • Der Geschäftsbereich ist akkreditiert nach ISO 17025 für alle relevanten Prüfverfahren im Bereich Immission und Emission. Fachbereich 20 - Zentrale Aufgaben • Probenentnahmen und Feldmessungen (ohne Messungen und Probenentnahmen im Rahmen der Radonberatung) u. a. Probenentnahmen aus Fließgewässern, Messung der nuklidspezifischen Gammaortsdosisleistung • Organisation und Logistik für die von externen Probenehmern gewonnenen und dem Geschäftsbereich 2 zu übergebenden Proben. Betrieb der Landesdatenzentrale und der Datenbank zur Umweltradioaktivität im Freistaat Sachsen • Unterstützung der beiden Landesmessstellen bei der Einführung und Pflege radiochemischer Verfahren Fachbereiche 21, 22 - Erste und Zweite Landesmessstelle für Umweltradioaktivität Laboranalysen • nach dem Strahlenschutzvorsorgegesetz • zur Überwachung der Wismut-Standorte • zur Überwachung des Forschungsstandort Rossendorf • zur Überwachung der Altstandorte des Uranbergbaus • zur Lebensmittelüberwachung • zu den aufsichtlichen Kontrolltätigkeiten des Sächsischen Landesamtes für Umwelt, Landwirtschaft und Geologie und des Sächsischen Staatsministeriums für Umwelt und Landwirtschaft u. a. in den Medien Wasser, Boden, Luft, Nahrungs- und Futtermittel. Analysierte Parameter: u. a. gamma- und alphastrahlende Radionuklide (z. B. Cäsium-137, Cobalt-60, Kalium-40, Uran-238); Strontium-90; Radium-226 und Radium-228). Fachbereich 23 - Immissionsmessungen Kontinuierliche Überwachung der Luftqualität durch Betrieb des stationären Luftmessnetzes des Freistaates (Online-Betrieb von 30 stationären Messstationen mit Übergabe der Messdaten ins Internet): • Laufende Messung der Luftgüteparameter SO2, NOx, Ozon, Benzol, Toluol, Xylole, Schwebstaub, Ruß • Gewinnung meteorologischer Daten zur Einschätzung der Luftgüteparameter • Sammlung von Schwebstaub (PM 10- und PM 2,5-Fraktionen) und Sedimentationsstaub zur analytischen Bestimmung von Schwermetallen, polyzyklischen Kohlenwasserstoffen (PAK) und Ruß • Absicherung der Messdatenverarbeitung und Kommunikation • Betreiben einer Messnetzzentrale, Plausibilitätskontrolle der Daten und deren Übergabe an das Landesamt für Umwelt, Landwirtschaft und Geologie und an die Öffentlichkeit • Absicherung und Überwachung der vorgegebenen Qualitätsstandards bei den Messungen durch den Betrieb eines Referenz- und Kalibrierlabors • Sicherung der Verfügbarkeit aller Messdaten zu > 95% • Weiterentwicklung des Luftmessnetzes entsprechend den gesetzlichen Anforderungen • Betreuung eines Depositionsmessnetzes (Niederschlag) mit zehn Messstellen • Betrieb von drei verkehrsnahen Sondermessstellen an hoch belasteten Straßen • Durchführung von Sondermessungen mit Immissionsmesswagen und mobilen Containern • Betrieb von Partikelmesssystemen im Submikronbereich (Zählung ultrafeiner Partikel) in Dresden • Betrieb von Verkehrszähleinrichtungen und Übernahmen dieser Verkehrszähldaten sowie von Pegelmessstellen der Städte in den Datenbestand des Luftmessnetzes Fachbereich 24 - Emissionsmessungen, Referenz- und Kalibrierlabor Der Fachbereich befasst sich mit der Durchführung von Emissionsmessungen an ausgewählten Anlagen aus besonderem Anlass im Auftrag des LfULG. Beispiele: • Emissionsmessungen an Blockheizkraftwerken in der Landwirtschaft (Geruch, Stickoxide, Gesamtkohlenstoff und Formaldehyd). • Ermittlung der Stickstoff-Deposition aus Tierhaltungsanlagen für Geflügel und Rinder (Emissionsmessungen von Ammoniak, Lachgas, Methan, Wasser, Kohlendioxid, Feuchte, Temperatur und Luftströmung , Ammoniak-Immissionsmessung mit DOAS-Trassenmesssystem). • Untersuchung von Emissionen aus holzgefeuerten Kleinfeuerungsanlagen zur Abschätzung von Auswirkungen der novellierten 1. BImSchV. • Unterstützung des LfULG bei der Überwachung bekannt gegebener Messstellen nach § 26 BImSchG.

Agroecological Transitions for Climate Adaptation and Mitigation

Globally, agriculture covers 40% of the earth’s surface and food systems are responsible for one-third of humanity’s contribution to global climate change. Yet, smallholder and subsistence farmers are among the most vulnerable to climate change, with extreme weather events and related food price volatility affecting livelihoods, biodiversity, and food security at multiple scales. This project builds on transdisciplinary research on agroecological transitions in vulnerable farming communities in Canada, Germany, India and Brazil. We will examine the influence of agroecological networks (farming organizations, institutional actors, and consumer groups) in promoting the perennialization of agriculture to support climate adaptation (improving resilience in livelihoods and food security) and mitigation (increasing carbon sequestration). Perennialization of agriculture integrates annual and perennial crops and trees into the same farming system. Compared to annual cropping systems which currently dominate global agriculture and markets, perennial crops show promise for climate adaptation and mitigation because of their contributions to carbon sequestration in tree biomass and soil organic carbon, and their buffering effects against soil degradation, drought, and other forms of extreme weather and climate variability. From a social wellbeing perspective, agroforestry and other diversified perennial systems offer opportunities to adapt to climate change and escape poverty traps, including higher and more stable farm incomes, balanced agricultural labour across growing seasons, improved working conditions compared to more input-intensive forms of agriculture and improved nutrition and health. Using a participatory action research approach, this project will use a novel methodology to test the relationships between personal, political, and practical leverage points driving the adoption of agroforestry and other practices supporting agricultural perennialization. We will sample farms and organizations in each case study across a diversification gradient from low-diversity farming systems to perennial and agroforestry-based management systems. We will then use qualitative and quantitative methods to assess climate resilience outcomes and estimate the potential of scaling adoption of perennial and agroforestry practices. A cross-case synthesis will take local institutional, environmental, and relational contexts into account to inform decision-making.

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