Propionate accumulates during start-up, restart and process imbalances in anaerobic biowaste digesters and rapidly decreases when obtaining steady-state conditions. In this project, propionate formation and degradation in biowaste (Karlsruhe) or market wastes (Bandung/Indonesia) in different anaerobic digesters or digestion regimes will be investigated. The influence of composition, pH, buffers and of (bioavailable) trace metals on propionate formation and degradation, shock-loading or restart of digesters will be investigated in Karlsruhe. Propionate degradation via the methylmalonyl-CoA or non-randomizing C6-dismutation pathway in different reactor systems and with different substrates will be investigated with pulse addition of specifically 14C-labeled propionate. Results should allow conclusions on the type of predominant propionate degraders. At LIPI, Bandung/Indonesia, the digestion of market waste suspensions in one stage or two-stage (acetogenic and methanogenic stage separated) CSTR reactor systems will be optimized and at ITB, Bandung/Indonesia, waste will first be separated into a liquid and solid phase and both fractions will then be digested either in a fixed-bed reactor (liquid) or in a dry fermentation system (solids). Such process alternatives are state of the art for wastewater treatment, but even in Europe experience with biowaste or biowaste fractions is scarce.
Die Biogasanlage Gebr. Kesseler Bioenergie GmbH, Scholzehof, 56826 Lutzerath beantragt die immissionsschutzrechtliche Genehmigung für die wesentliche Änderung einer Anlage zur biologischen Behandlung von Gülle, soweit die Behandlung aus-schließlich zur Verwertung durch anaerobe Vergärung (Biogaserzeugung) dient (hier: Biogasanlage) durch Errichtung und Betrieb eines gasdichten Gärrestlagers II inkl. Abfüllfläche II, Errichtung und Betrieb einer Separation für Rindergülle und für Gärreste, Umstellung auf Trockenfermentation, Erweiterung der Positivkataloges, Erhöhung der Durchsatzkapazität und der Gasproduktion sowie der Gasspeicherkapazität und die Errichtung eines Havariewalles auf dem Betriebsgelände in 56825 Schmitt (Gemarkung Schmitt, Flur 5, Flurstücke 29/6 und 29/7).
Aim: Valorisation of side-streams of the Citrus industry using the genetic diversity of monokarya from the basidiomycete Pleurotus sapidus. The genetic diversity of the basidiospores of Pleurotus sapidus (MKs) obtained from two dikaryotic strains of P. sapidus (Dk421 and Dk3174) will be exploited. Mks with high growth rate on milled Citrus peel, pulp and seed of orange, tangerine, lemon will be selected and grown as solid state and submerged fermentation (SF). Metabolites will be extracted and evaluated for biological activities. Samples before and after the fungal transformation taken from SSF and SF cultures will be analysed. Rapid product analyses using TLC and established coupled HPLC-DAD-ELSD will focus on the most promising strains. Specific targets are flavonoids with an increased number of hydroxyl groups on the B-ring, unsaturated carbonyls and terpenoids from the oxo-functionalisation of limonene, citronellal and farnesene isomers. High resolution and multi-dimensional GC-MS and multireaction monitoring (varying MS collision energies) will be used. Extracts from various strain/culture combinations (SSF or SF) will be lyophilized. One fraction of each sample will be tested for its biopesticide action, and another one for its quality as a feed supplement. SSF will be carried out in a rotary drum solid-substrate fermentation system.
The project is comprised of seven major work packages: 1. Generation and selection of the monokaryons (CITER) 2. Growth of the monokaryons (CITER) 3. Selection of the optimal culture conditions to obtain bioactive compounds using the selected Mk form step 2. (CITER, LUH, JLU, JUB) 4. Analytical evaluation of the biotransformation/conversion products (LUH, JLU) 5. Automated screening of Mks by chiral GC-GC (JLU) 6. Bioactivity test of crude extracts obtained from SSF and SF (IMBIV, IIB) 7. Bioprocess design and scale-up (JLU, JUB).