Chlorinated ethylenes are prevalent groundwater contaminants. Numerous studies have addressed the mechanism of their reductive dehalogenation during biodegradation and reaction with zero-valent iron. However, despite insight with purified enzymes and well-characterized chemical model systems, conclusive evidence has been missing that the same mechanisms do indeed prevail in real-world transformations. While dual kinetic isotope effect measurements can provide such lines of evidence, until now this approach has not been possible for chlorinated ethylenes because an adequate method for continuous flow compound specific chlorine isotope analysis has been missing. This study attempts to close this prevalent research gap by a combination of two complementary approaches. (1) A novel analytical method to measure isotope effects for carbon and chlorine. (2) A carefully chosen set of well-defined model reactants representing distinct dehalogenation mechanisms believed to be important in real-world systems. Isotope trends observed in biotic and abiotic environmental dehalogenation will be compared to these model reactions, and the respective mechanistic hypotheses will be confirmed or discarded. With this hypothesis-driven approach it is our goal to elucidate for the first timdehalogenation reactions.
BACKGROUND: The Kingdom of Jordan belongs to the ten water scarcest countries in the world, and climate change is likely to increase the frequency of future droughts. Jordan is considered among the 10 most water impoverished countries in the world, with per capita water availability estimated at 170 m per annum, compared to an average of 1,000 m per annum in other countries. Jordan Government has taken the strategic decision to develop a conveyor system including a 325 km pipe to pump 100 million cubic meters per year of potable water from Disi-Mudawwara close to the Saudi Border in the south, to the Greater Amman area in the north. The construction of the water pipeline has started end of 2009 and shall be finished in 2013. Later on, the pipeline could serve as a major part of a national water carrier in order to convey desalinated water from the Red Sea to the economically most important central region of the country. The conveyor project will not only significantly increase water supplies to the capital, but also provide for the re-allocation of current supplies to other governorates, and for the conservation of aquifers. In the context of the Disi project that is co-funded by EIB two Environmental and Social Management Plans have been prepared: one for the private project partners and one for the Jordan Government. The latter includes the Governments obligation to re-balance water allocations to irrigation and to gradually restore the protected wetlands of Azraq (Ramsar site) east of Amman that has been depleted due to over-abstraction by re-directing discharge of highland aquifers after the Disi pipeline becomes operational. The Water Strategy recognizes that groundwater extraction for irrigation is beyond acceptable limits. Since the source is finite and priority should be given to human consumption it proposes to tackle the demand for irrigation through tariff adjustments, improved irrigation technology and disincentive to water intensive crops. The Disi aquifer is currently used for irrigation by farms producing all kinds of fruits and vegetables on a large scale and exporting most of their products to the Saudi and European markets and it is almost a third of Jordan's total consumption. The licenses for that commercial irrigation were finished by 2011/12. Whilst the licenses will be not renewed the difficulty will be the enforcement and satellite based information become an important supporting tool for monitoring. OUTLOOK: The ESA funded project Water management had the objective to support the South-North conveyor project and the activities of EIB together with the MWI in Jordan to ensure the supply of water for the increasing demand. EO Information provides a baseline for land cover and elevation and support the monitoring of further stages. usw.
Bamboos (Poaceae) are widespread in tropical and subtropical forests. Particularly in Asia, bamboos are cultivated by smallholders and increasingly in large plantations. In contrast to trees, reliable assessments of water use characteristics for bamboo are very scarce. Recently we tested a set of methods for assessing bamboo water use and obtained first results. Objectives of the proposed project are (1) to further test and develop the methods, (2) to compare the water use of different bamboo species, (3) to analyze the water use to bamboo size relationship across species, and (4) to assess effects of bamboo culm density on the stand-level transpiration. The study shall be conducted in South China where bamboos are very abundant. It is planned to work in a common garden (method testing), a botanical garden (species comparison, water use to size relationship), and on-farm (effects of culm density). Method testing will include a variety of approaches (thermal dissipation probes, stem heat balance, deuterium tracing and gravimetry), whereas subsequent steps will be based on thermal methods. The results may contribute to an improved understanding of bamboo water use characteristics and a more appropriate management of bamboo with respect to water resources.
Farm structures are often characterized by regional heterogeneity, agglomeration effects, sub-optimal farm sizes and income disparities. The main objective of this study is to analyze whether this is a result of path dependent structural change, what the determinants of path dependence are, and how it may be overcome. The focus is on the German dairy sector which has been highly regulated and subsidized in the past and faces severe structural deficits. The future of this sector in the process of an ongoing liberalization will be analyzed by applying theoretical concepts of path dependence and path breaking. In these regards, key issues are the actual situation, technological and market trends as well as agricultural policies. The methodology will be based on a participative use of the agent-based model AgriPoliS and participatory laboratory experiments. On the one hand, AgriPoliS will be tested as a tool for stakeholder oriented analysis of mechanisms, trends and policy effects. This part aims to analyze whether and how path dependence of structural change can be overcome on a sector level. In a second part, AgriPoliS will be extended such that human players (farmers, students) can take over the role of agents in the model. This part aims to compare human agents with computer agents in order to overcome single farm path dependence.
It has been suggested that dying and decaying fine roots and root exudation represent important, if not the most important, sources of soil organic carbon (SOC) in forest soils. This may be especially true for deep-reaching roots in the subsoil, but precise data to prove this assumption are lacking. This subproject (1) examines the distribution and abundance of fine roots (greater than 2 mm diameter) and coarse roots (greater than 2 mm) in the subsoil to 240 cm depth of the three subsoil observatories in a mature European beech (Fagus sylvatica) stand, (2) quantifies the turnover of beech fine roots by direct observation (mini-rhizotron approach), (3) measures the decomposition of dead fine root mass in different soil depths, and (4) quantifies root exudation and the N-uptake potential with novel techniques under in situ conditions with the aim (i) to quantify the C flux to the SOC pool upon root death in the subsoil, (ii) to obtain a quantitative estimate of root exudation in the subsoil, and (iii) to assess the uptake activity of fine roots in the subsoil as compared to roots in the topsoil. Key methods applied are (a) the microscopic distinction between live and dead fine root mass, (b) the estimation of fine and coarse root age by the 14C bomb approach and annual ring counting in roots, (c) the direct observation of the formation and disappearance of fine roots in rhizotron tubes by sequential root imaging (CI-600 system, CID) and the calculation of root turnover, (d) the measurement of root litter decomposition using litter bags under field and controlled laboratory conditions, (e) the estimation of root N-uptake capacity by exposing intact fine roots to 15NH4+ and 15NO3- solutions, and (f) the measurement of root exudation by exposing intact fine root branches to trap solutions in cuvettes in the field and analysing for carbohydrates and amino acids by HPLC and Py-FIMS (cooperation with Prof. A. Fischer, University of Trier). The obtained data will be analysed for differences in root abundance and activity between subsoil (100-200 cm) and topsoil (0-20 cm) and will be related to soil chemical and soil biological data collected by the partner projects that may control root turnover and exudation in the subsoil. In a supplementary study, fine root biomass distribution and root turnover will also be studied at the four additional beech sites for examining root-borne C fluxes in the subsoil of beech forests under contrasting soil conditions of different geological substrates (Triassic limestone and sandstone, Quaternary sand and loess deposits).
Dairy farming across Germany displays diverse production systems. Factor endowment, management, technology adoption as well as competitive dynamics in the local or regional land, agribusiness and dairy processing sectors contribute to this differentiation on farm level. These differences impact on the ability of dairy farms and regional dairy production systems to successfully respond to pressures arising from future market and policy changes. The overall objective of the research activities of which this project is a part of, is to develop a thorough understanding of the processes that govern the spatial dynamics of dairy farm development in different regions in Germany. The central hypothesis of this research project is that management system and technological choices differ systematically across local production and market conditions. The empirical approach will focus on the estimation of farm specific nonparametric cost functions for dairy farms located in across Germany differentiated by time and location. A spatially differentiated data base with information on input use, resource availability, as well as local market conditions for land and output markets will be compiled. The nonparametric approach is specifically suited to disclose a more accurate representation of dairy production system heterogeneity across locations and time compared to parametric concepts as it provides the necessary flexibility to accommodate non-linearities relevant for a wide domain of explanatory variables. The methodology employed goes beyond the state of the art of the literature as it combines kernel density estimation with a Bayesian sampling approach to provide theory consistent parameters for each farm in the data sample.The specific methodological hypothesis is that the nonparametric approach is superior to current parametric techniques and this hypothesis is tested using statistical model evaluation. Regarding the farm management and technological choices, we hypothesize that land suitability for feed production determines the farm intensity of dairy production and thus management and technological choices. With respect to the ability of farms to successfully respond to market pressures we hypothesize that farms at the upper and lower tail of the intensity distribution both can generate positive returns from dairy production. These last two hypotheses will be tested using the estimated spatially differentiated farm specific costs and marginal costs.The expected outcomes are of relevance for the agricultural sector and the food supply chain economy as a whole as fundamental market structure changes in the dairy sector are ongoing due to the abolition of the quota regulation in the years 2014/2015. Thus, exact knowledge about differences and development of dairy cost heterogeneity of farms within and between regions are an important factor for the actors involved in the market as well as the political support of this process.
In many plant species, FLOWERING LOCUS T and related proteins are the mobile signal that communicates information on photoperiod from the leaves to the shoots, where the transition to flowering is realized. FT expression is tightly controlled at the transcriptional level so that it is restricted to leaves, occurs only in appropriate photoperiods, and integrates ambient temperature and developmental cues, as well as information on biotic and abiotic stress. We previously established that FT transcription in the model plant Arabidopsis thaliana requires proximal promoter cis-elements and a distal enhancer, both evolutionary conserved among Brassicacea species. In addition, FT transcription is blocked prior vernalization in biannual accessions and vernalization-dependency of FT is controlled through a CArG-box located in the first intron that binds the transcriptional repressor FLOWERING LOCUS C (FLC). Chromatin-mediated repression by the Polycomb Group (PcG) pathway is required for photoperiod-dependent FT regulation and participates in FT expression level modulation in response to other cues.In this project, I propose to explore the available sequence data from the 1001 genome project in Arabidopsis to evaluate how often changes in regulatory cis-elements at FT have occurred and how these translate into an adaptive value. Allele-specific FT expression pattern will be measured in F1 hybrids of different accessions in response to varying environmental conditions. FT alleles that show cis-regulatory variation will be further analyzed to pinpoint the causal regulatory changes and study their effect in more detail. The allotetrapolyploid species Brassica napus is a hybrid of two Brassiceae species belonging to the A- and C-type genome, which are in turn mesopolyploid due to a genome triplication that occurred ca. 10x106 years ago. We will determine allele-specific expression of FT paralogs from both genomes of a collection of B. napus accessions. The plants will be grown in the field in changing environmental conditions to maximize the chance to detect expression variation of the paralogs. We will compare the contribution of the founder genomes to the regulation of flowering time and asses variation in this contribution. A particular focus will be to study the impact of chromatin-mediated repression on allele selection in B. napus.
Steroid hormones are essential in orchestrating oocyte maturation, i.e. estrogens of follicular origin support the development of the female gamete and fertilization. In this project the concentration of free and conjugated estrogens during follicular development will be analysed and compared to local concentrations in the developing follicle. Cattle are suitable animal models because of the accessibility and suitability for frequent examination and sampling. Furthermore, it has been useful for understanding several features of human reproduction including follicular dynamics, the fate of the emerging follicles is orchestrated mainly by gonadotropins and steroid hormones in a similar manner. Ovarian SULT1E1 participates locally in the regulation of follicular estrogen activity. The ESTcatalysed down-regulation of estrogen activity enables normal ovulation. Conversely, sulfoconjugated estrogens may also be precursors of the production of free estrogens depending on estrogen sulfatase (StS) acitivity. In mammals, follicular luteinisation/ovulation is triggered by a surge in LH and is characterised by numerous physical and biochemical changes, including the decreased production of estradiol (E2). This loss in E2 biosynthetic capacity has been explained by a marked decrease in the expression of key steroidogenic enzymes involved in the follicular production of active estrogens. However, little is known about the regulation of enzymes/proteins responsible for the inactivation and elimination of estrogens, as mediated for example by EST during this period.
Perennial fodder cropping potentially increases subsoil biopore density by formation of extensive root systems and temporary soil rest. We will quantify root length density, earthworm abundance and biopore size classes after Medicago sativa, Cichorium intybus and Festuca arundinacea grown for 1, 2 and 3 years respectively in the applied research unit's Central Field Trial (CeFiT) which is established and maintained by our working group. Shoot parameters including transpiration, gas exchange and chlorophyll fluorescence will frequently be recorded. Precrop effects on oilseed rape and cereals will be quantified with regard to crop yield, nutrient transfer and H2-release. The soil associated with biopores (i.e. the driloshpere) is generally rich in nutrients as compared to the bulk soil and is therefore supposed to be a potential hot spot for nutrient acquisition. However, contact areas between roots and the pore wall have been reported to be low. It is still unclear to which extent the nutrients present in the drilosphere are used and which potential relevance subsoil biopores may have for the nutrient supply of crops. We will use a flexible videoscope to determine the root-soil contact in biopores. Nitrogen input into the drilosphere by earthworms and potential re-uptake of nitrogen from the drilosphere by subsequent crops with different rooting systems (oilseed rape vs. cereals) will be quantified using 15N as a tracer.
Beach sand deposits are widespread in the area around Sandefjord, at the western coast of the Oslofjord, southern Norway. The age of the deposits continuously increases with elevation, as the area has been subject to steady glacio-isostatic uplift throughout the Holocene. Existing local sea level curves provide age control related to elevation. Thus, the area offers excellent conditions to test hypotheses on soil formation and OSL dating. A chronosequence covering the last 10 000 years will be established. A preliminary study showed that soil formation leads to Podzols within 4300 - 6600 years. Micromorphological analyses suggest that clay illuviation takes place before and below podzolisation. It is hypothesised that clay translocation goes on contemporarily with podzolisation, but at greater soil depth, where the chemical conditions are suitable. This hypothesis will be proved by more detailed micromorphological investigation and chemical analyses. The factors controlling soil forming processes and their rates, will be determined by analyzing elemental composition, primary minerals and clay mineralogy. Preliminary OSL dating tests suggest that the beach sand deposits are OSL dateable despite the high latitude. This hypothesis will be checked by comparing OSL datings to ages derived from the 14C-based sea level curves.
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