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Human influences on forests in southern Ethiopia: the case of Shashemane-Munessa-forest

Especially during the last decades, the natural forests of Ethiopia have been heavily disturbed by human activities. Some forests have been totally cleared and converted into fields for agricultural use, other suffered from different influences, such as heavy grazing and selective logging. The ongoing research in the Shashemane-Munessa-study area (Gu 406/8-1,2) showed clearly that, in spite of interdiction and control, forests continue to be cleared and degraded. However, it is not yet sufficiently known, how and why these processes are still going on. Growing population pressure and economic constraints for the people living in and around the forests contribute to the actual situation but allow no final answers to the complex situation. Concerning a sustainable management of the forests there is to no solid basis for recommendations from the socioeconomic and socio-cultural view. Therefore, a comprehensive analysis of the traditional needs and forms of forest use, including all forest products, is necessary. The objective of this project is, to achieve this basis by carrying out intensive field observations, the consultation of aerial photographs, satellite imagery and above all semi-structured interviews with the population in the study area in order to contribute to the recommendations for a sustainable use of the Munessa Shasemane forests.

Stakeholders, Interests and Power as Drivers of Community Forestry: Comparative Analysis of Albania, Germany, Cameroon, Indonesia, Namibia, Nepal and Thailand

Community forestry has not met the great public expectations on a significant contribution to sustainable forestry yet. Recent research in the management and policy of community forestry describes a complex process of multi level social choice which determines the outcomes. Our hypothesis is that the key factors determining the outcomes of community forestry are the interests and power of the external stake holders. This hypothesis will be tested in a comparative quantitative and qualitative analysis. In seven countries comprising developed and developing countries 84 cases will be used for comparison. The comparative analysis will be carried out by one PhD student financed by the project. He will do the field work in close cooperation with PhD students who are already conducting their PhD analysis the different countries. The comparative analysis is aimed to explore key drivers of community forestry which are not yet identified in literature.

Simulated field environment with combined salt and drought stresses as a platform for phenotyping plant tolerance to salinity

Salinity occurs often simultaneously with drought stress. Therefore, breeding for tolerance to combined both stresses can contribute significantly to crop yield. However, classical selection in salinity has generally been unsuccessful, partly due to high variability of salt stress resulting from the different salinity and drought status. Unfortunately, the use of unrealistic stress protocols for mimicking salinity and drought stress is the norm rather than the exception in biotechnological studies. Therefore, the great challenge is to gain knowledge required to develop plants with enhanced tolerance to field conditions. Our overall hypothesis is that a realistic stress protocol simulating a field environment with combined salt and drought stress as a platform for precision phenotyping of plant tolerance to salinity may solve this problem. This study will demonstrate that highly managed stress environments can be created and key traits of plants can be characterised by using advanced non-destructive sensors that are able to identify relevant traits of plants.

Linking soil architecture formation with changing permafrost regime to carbon turnover in high latitude soils at multiple spatial scales

Most soils develop distinct soil architecture during pedogenesis and soil organic carbon (SOC) is sequestered within a hierarchical system of mineral-organic associations and aggregates. Permafrost soils store large amounts of carbon due to their permanently frozen subsoil and a lack of oxygen in the active layer, but they lack complex soil structure. With permafrost thaw more oxidative conditions and increasing soil temperature presumably enhance the build-up of more complex units of soil architecture and may counterbalance, at least partly, SOC mineralization. We aim to explore the development of mineral-organic associations and aggregates under different permafrost impact with respect to SOC stabilization. This information will be linked to environmental control factors relevant for SOC turnover at the pedon and stand scale to bridge processes occurring at the aggregate scale to larger spatial dimensions. We will combine in situ spectroscopic techniques with fractionation approaches and identify mechanisms relevant for SOC turnover at different scales by multivariate statistics and variogram analyses. From this we expect a deeper knowledge about soil architecture formation in the transition of permafrost soils to terrestrial soils and a scale-spanning mechanistic understanding of SOC cycling in permafrost regions.

Effects of water content, input of roots and dissolved organic matter and spatial inaccessibility on C turnover & determination of the spatial variability of subsoil properties

It is well established that reduced supply of fresh organic matter, interactions of organic matter with mineral phases and spatial inaccessibility affect C stocks in subsoils. However, quantitative information required for a better understanding of the contribution of each of the different processes to C sequestration in subsoils and for improvements of subsoil C models is scarce. The same is true for the main controlling factors of the decomposition rates of soil organic matter in subsoils. Moreover, information on spatial variabilities of different properties in the subsoil is rare. The few studies available which couple near and middle infrared spectroscopy (NIRS/MIRS) with geostatistical approaches indicate a potential for the creation of spatial maps which may show hot spots with increased biological activities in the soil profile and their effects on the distribution of C contents. Objectives are (i) to determine the mean residence time of subsoil C in different fractions by applying fractionation procedures in combination with 14C measurements; (ii) to study the effects of water content, input of 13C-labelled roots and dissolved organic matter and spatial inaccessibility on C turnover in an automatic microcosm system; (iii) to determine general soil properties and soil biological and chemical characteristics using NIRS and MIRS, and (iv) to extrapolate the measured and estimated soil properties to the vertical profiles by using different spatial interpolation techniques. For the NIRS/MIRS applications, sample pretreatment (air-dried vs. freeze-dried samples) and calibration procedures (a modified partial least square (MPLS) approach vs. a genetic algorithm coupled with MPLS or PLS) will be optimized. We hypothesize that the combined application of chemical fractionation in combination with 14C measurements and the results of the incubation experiments will give the pool sizes of passive, intermediate, labile and very labile C and N and the mean residence times of labile and very labile C and N. These results will make it possible to initialize the new quantitative model to be developed by subproject PC. Additionally, we hypothesize that the sample pretreatment 'freeze-drying' will be more useful for the estimation of soil biological characteristics than air-drying. The GA-MPLS and GA-PLS approaches are expected to give better estimates of the soil characteristics than the MPLS and PLS approaches. The spatial maps for the different subsoil characteristics in combination with the spatial maps of temperature and water contents will presumably enable us to explain the spatial heterogeneity of C contents.

Schwerpunktprogramm (SPP) 1158: Antarctic Research with Comparable Investigations in Arctic Sea Ice Areas; Bereich Infrastruktur - Antarktisforschung mit vergleichenden Untersuchungen in arktischen Eisgebieten, Biogene Opalisotope - neue Proxies zur Untersuchung vergangener Nährstoffkreisläufe und hydrographischer Strukturen im Südpazifik in Beziehung zu der Entwicklung des Klimas und der antarktischen Kryosphäre

Der Verlauf der atmosphärischen CO2-Konzentrationen während der vergangenen Klimazyklen ist durch ein Sägezahnmuster mit Maxima in Warmzeiten und Minima in Kaltzeiten geprägt. Es besteht derzeit Konsens, dass insbesondere der Süd Ozean (SO) eine Schlüsselfunktion bei der Steuerung der CO2-Entwicklung einnimmt. Allerdings sind die dabei wirksamen Mechanismen, die in Zusammenhang mit Änderungen der Windmuster, Ozeanzirkulation, Stratifizierung der Wassersäule, Meereisausdehnung und biologischer Produktion stehen, noch nicht ausreichend bekannt. Daten zur Wirkung dieser Prozesse im Wechsel von Warm- und Kaltzeiten beziehen sich bislang fast ausschließlich auf den atlantischen SO. Um ein umfassendes Bild der Klimasteuerung durch den SO zu erhalten muss geklärt werden, wie weit sich die aus dem atlantischen SO bekannten Prozesswirkungen auf den pazifischen SO übertragen lassen. Dies ist deshalb von Bedeutung, da der pazifische SO den größten Teil des SO einnimmt. Darüber hinaus stellt er das hauptsächliche Abflussgebiet des Westantarktischen Eisschildes (WAIS) in den SO dar. Im Rahmen des Projektes sollen mit einer neu entwickelten Proxy-Methode Paläoumwelt-Zeitreihen an ausgewählten Sedimentkernen von latitudinalen Schnitten über den pazifischen SO hinweg gewonnen werden. Dabei handelt es sich um kombinierte Sauerstoff- und Siliziumisotopenmessungen an gereinigten Diatomeen und Radiolarien. Es sollen erstmalig die physikalischen Eigenschaften und Nährstoffbedingungen in verschiedenen Stockwerken des Oberflächenwassers aus verschiedenen Ablagerungsräumen und während unterschiedlicher Klimabedingungen beschrieben werden. Dies umfasst Bedingungen von kälter als heute (z.B. Letztes Glaziales Maximum) bis zu wärmer als heute (z.B. Marines Isotopen Stadium, MIS 5.5). Die Untersuchungen geben Hinweise zur (1) Sensitivität des antarktischen Ökosystems auf den Eintrag von Mikronährstoffen (Eisendüngung), (2) Oberflächenwasserstratifizierung und (3) 'Silicic-Acid leakage'-Hypothese, und tragen damit zur Überprüfung verschiedener Hypothesen zur Klimawirksamkeit von SO-Prozessen bei. Die neuen Proxies bilden überdies Oberflächen-Salzgehaltsanomalien ab, die Hinweise zur Stabilität des WAIS unter verschiedenen Klimabedingungen geben. Darüber hinaus kann die Hypothese getestet werden, nach der der WAIS während MIS 5.5 vollständig abgebaut war. Die Projektergebnisse sollen mit Simulationen mit einem kombinierten biogeochemischen (Si-Isotope beinhaltenden) Atmosphäre-Ozean-Zirkulations-Modell aus einem laufenden SPP1158-DFG Projekt an der CAU Kiel (PI B. Schneider) verglichen werden. Damit sollen die jeweiligen Beiträge der Ozeanzirkulation und der biologischen Produktion zum CO2-Austausch zwischen Ozean und Atmosphäre getrennt und statistisch analysiert werden. Informationen zu Staubeintrag, biogenen Flussraten, physikalischen Ozeanparametern und zur Erstellung von Altersmodellen stehen durch Zusammenarbeit mit anderen (inter)nationalen Projekten zur Verfügung.

Population biology and genetics of dark septate endophytes (DSE)

We study population biology and life histories of DSE. Dark septate root endophytes (DSE) are ubiquitous fungal tree root colonizers in temperate and boreal conifer forest ecosystems. The supposedly asexual Phialocephala fortinii was identified as the main component of these DSE but constitutes a species complex in its own right. Species in this complex are morphologically indistinguishable with one exceptition; Acephala applanata was described as a new species which is characterized by the absence of aerial mycelium and slow growth rate. Application of biological, phylogenetic and population genetic species concepts will allow to discriminate additional species. The experimental programme is multi-disciplinary in approach, utilizing classical mycological and molecular genetic techniques.

Unraveling the genetic architecture of winter hardiness and quality traits in durum by genome wide and canidate gene based association mapping

Durum wheat is mainly grown as a summer crop. An introduction of a winter form failed until now due to the difficulty to combine winter hardiness with required process quality. Winter hardiness is a complex trait, but in most regions the frost tolerance is decisive. Thereby a major QTL, which was found in T. monococcum, T.aestivum, H. vulgare and S.cereale on chromosome 5, seems especially important. With genotyping by sequencing it is now possible to make association mapping based on very high dense marker maps, which delivers new possibilities to detect main and epistatic effects. Furthermore, new sequencing techniques allow candidate gene based association mapping. The main aim of the project is to unravel the genetic architecture of frost tolerance and quality traits in durum. Thereby, the objectives are to (1) determine the genetic variance, heritability and correlations among frost tolerance and quality traits, (2) examine linkage disequilibrium and population structure, (3) investigate sequence polymorphism at candidate genes for frost tolerance, and (4) perform candidate gene based and genome wide association mapping.

Ökologische Untersuchungen in einem Regenwald (Complex Mesophyll Vine Forest) in Nordost Queensland, Australien

Neben Studien zur Diversität von Gefäßpflanzen in ausgewählten Untersuchungsflächen wurde der Erforschung der endemischen Palmenart Normanbya normanbyi besondere Aufmerksamkeit gewidmet. Im Mittelpunkt stehen Studien zur Blüten- und Fruchtökologie. Dabei wurde u.a. eine bisher unbeschriebene Gattung der Gallmücken entdeckt. Der interdisziplinäre Forschungsansatz soll wesentliche Erkenntnisse über Tier-Pflanze-Interaktionen in Regenwäldern liefern. Hauptaugenmerk liegt auf der Erfassung der Blüten- und Fruchtphänologie eines tropischen Tieflandregenwaldes in Nordostqueensland. Das Projekt beinhaltet die Erfassung der Phänologie aller Unterwuchsarten, Lianen und Baumarten in einer 1 ha großen Untersuchungsfläche. Weiterführend wird die Blühphänologie einer ausgewählten Palmenart Normanbay normanbyi erfasst und mit Hilfe molekulargenetischer Methoden im Zusammenhang mit der Verwandtschaftsstruktur ausgewählter Populationen betrachtet. Die Feldarbeiten für das 2003 begonnene Projekt wurden im Juli 2005 abgeschlossen.

Species discrimination of plant roots by Fourier transform infrared (FTIR) spectroscopy

Comprehension of belowground competition between plant species is a central part in understanding the complex interactions in intercropped agricultural systems, between crops and weeds as well as in natural ecosystems. So far, no simple and rapid method for species discrimination of roots in the soil exists. We will be developing a method for root discrimination of various species based on Fourier Transform Infrared (FTIR)-Attenuated Total Reflexion (ATR) Spectroscopy and expanding its application to the field. The absorbance patterns of FTIR-ATR spectra represent the chemical sample composition like an individual fingerprint. By means of multivariate methods, spectra will be grouped according to spectral and chemical similarity in order to achieve species discrimination. We will investigate pea and oat roots as well as maize and barnyard grass roots using various cultivars/proveniences grown in the greenhouse. Pea and oat are recommendable species for intercropping to achieve superior grain and protein yields in an environmentally sustainable manner. To evaluate the effects of intercropping on root distribution in the field, root segments will be measured directly at the soil profile wall using a mobile FTIR spectrometer. By extracting the main root compounds (lipids, proteins, carbohydrates) and recording their FTIR-ATR spectra as references, we will elucidate the chemical basis of species-specific differences.

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