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Measurements of the marine carbon system from different approaches, discrete sampling and sensors during July 2023 in a Mesocosm Facility, Wilhelmshaven, Germany

The file contains data from the Marine Carbon System. It gathered parameters from the inorganic carbon and incorporate the organic alkalinity as a main contributor to the sea surface microlayer (SML) compared to the Underlaying Water (ULW). Data was collected during Mesocosm Study from 18-May to 17-July 2024 in the Sea sURface Facility (SURF), Institute for Chemistry and Biology of the Marine Environment, Wilhelmshaven, Germany. Discrete samples to measure Dissolved Inorganic Carbon (DIC), Total Alkalinity (TA) and Organic Alkalinity (OA) were collected. For SML data, DIC, TA and OA was collected every third day following the glass‑plate technique (Harvey and Burzell, 1972). The ULW data, DIC, TA and OA were collected every day using a suction system to collect the sample from 0.4 m depth. Discrete samples were transported to the laboratory for further analysis; DIC was determined coulometrically (CM5017, UIC, IL, USA), and TA concentration was directly measured by high-precision closed-cell potentiometric titration (916 Ti-Touch, Metrohm, Switzerland). OA concentration was determined directly after TA was measured, using the same sample (from which all carbonate species had been purged), denoted as back titration. The dataset includes quality flags 0-4 with flags 1 and 2 are ready for use. See metadata for more information.

Physiological responses of Mytilus edulis and Magallana gigas from a three-month mesocosm experiment, Sylt, 2023

This dataset reports physiological measurements of two bivalve species, Mytilus edulis (blue mussel) and Magallana gigas (Pacific oyster), obtained during a three-month mesocosm experiment conducted in Sylt, Germany, in 2023. Physiological data were collected between 27 April 2023 and 25 June 2023.Twelve mesocosms were used to investigate the effects of temperature on individual-level physiological traits, with treatments including ambient temperature and ambient +3°C. Parameters measured include clearance rate, ingestion rate, and respiration rate. Environmental variables such as water temperature, depth, and sampling time were recorded for each measurement. Individual bivalves were labeled for tracking, and species identification followed WoRMS taxonomy. Measurements were performed using handheld multiparameter instruments and laboratory analyses. The dataset provides high-resolution, individual-based physiological responses of bivalves to moderate warming, supporting research on temperature-dependent feeding, metabolic processes, and energy flux in coastal ecosystems.

Moisture, temperature and light modualte methane oxidation from dry riverbeds - A mesocosms experiment

In this study, under lab conditions (Jan-Apr 2022) in we examined how three key drivers of methane oxidation in soils also modulate methane oxidation (i.e. methanotrophy) in dry riverbed sediments: gravimetric water content (GWC, as proxy of moisture), temperature, and light quality and intensity. We measured potential methane oxidation rates (PMO) as proxy of methanotrophy across a gradient of GWC, temperature (10, 20 and 30ºC), and under different light conditions (green and growth light) from dry (8% GWC) and wet (100%GWC) sediments collected in early Spring 2022 in the Queich River in Offenbach an der Queich (Germany). We used incubation vials (125mL) with about 10 mL sediment and spiked it with 120uL of pure methane gas following Bodmer et al. (2020). Incubations lasted for a week (until methane concentrations were below detection limit) and we replicated each treatment level four times (except for 10ºC that had just three).

KOSMOS 2023 Helgoland mesocosm study on ocean alkalinity enhancement: sediment trap particle flux data and water column biogeochemistry

The data presented herein originates from a mesocosm study conducted as part of the BMBF CDRmare, Retake project (grant agreement no. 03F0895A), aimed at investigating the ecological ramifications of ocean alkalinity enhancement (OAE). Twelve mesocosms were deployed in Helgoland South Harbor, Germany, and systematically sampled using integrated water samplers over the period spanning from March 12th to April 20th, 2023. Six alkalinity levels under two dilution scenarios were established to differentiate between localized and uniform OAE additions. Alkalinity was increased stepwise to ΔTAmax = 1250 μmol kg-1 (250 μmol TA kg-1 increments) using sodium hydroxide (NaOH) with calcium chloride (CaCl2) to simulate cation release during calcium-based mineral dissolution, causing strong carbonate chemistry perturbations (e.g., pHT > 9.25). The dataset encompasses a spectrum of sediment trap particle flux data, water column biogeochemistry including pigment variables, inorganic nutrients, carbonate chemistry parameters. The study and data set offer insights into impacts of alkalinity enhancement on marine ecosystems and their associated biogeochemistry.

Plant growth and biomass measurements from a mesocosm experiment on the effects of sediment properties and warming on the salt-marsh pioneer Salicornia, Sylt, Germany

This dataset contains plant growth and biomass measurements collected during a controlled mesocosm experiment conducted at the AWI Sylt Outdoor Mesocosm facility, Wadden Sea Station Sylt, Germany, between June and September 2025. The experiment investigated the effects of sediment grain size, microtopography and climate warming on the salt marsh pioneer Salicornia. Plants were grown in PVC tubes filled with sandy, mixed or muddy sediments and exposed to four different climate treatments (ambient, warming, warming-drought, heatwave-drought). Climate treatments were implemented through active control of the water temperature. Experimental microtopographies simulated small-scale elevation differences. Plant height was measured repeatedly to quantify growth dynamics. Above ground biomass was measured at the end of the experiment. The experiment was conducted under controlled mesocosm conditions to quantify the relative importance of sediment properties, topographic variation and warming for pioneer marsh vegetation performance.

Water temperature measurements from a mesocosm experiment on the effects of sediment properties and warming on the salt-marsh pioneer Salicornia, Sylt, Germany

This dataset contains continuous water temperature measurements collected during a controlled mesocosm experiment conducted at the AWI Sylt Outdoor Mesocosm facility, Wadden Sea Station Sylt, Germany, between June and September 2025. The experiment investigated the effects of sediment grain size, microtopography and climate warming on the salt marsh pioneer Salicornia. Plants were grown in PVC tubes filled with sandy, mixed or muddy sediments and exposed to four different climate treatments (ambient, warming, warming-drought, heatwave-drought). Climate treatments were implemented through active control of the water temperature. Experimental microtopographies simulated small-scale elevation differences. The experiment was conducted under controlled mesocosm conditions to quantify the relative importance of sediment properties, topographic variation and warming for pioneer marsh vegetation performance.

Air temperature and relative humidity measurements from a mesocosm experiment on the effects of sediment properties and warming on the salt-marsh pioneer Salicornia, Sylt, Germany

This dataset contains continuous air temperature and relative humidity measurements collected during a controlled mesocosm experiment conducted at the AWI Sylt Outdoor Mesocosm facility, Wadden Sea Station Sylt, Germany, between June and September 2025. The experiment investigated the effects of sediment grain size, microtopography and climate warming on the salt marsh pioneer Salicornia. Plants were grown in PVC tubes filled with sandy, mixed or muddy sediments and exposed to four different climate treatments (ambient, warming, warming-drought, heatwave-drought). Climate treatments were implemented through active control of the water temperature. Air temperature was not directly manipulated but responded indirectly to the imposed water temperature treatments and ambient atmospheric conditions. Experimental microtopographies simulated small-scale elevation differences. The experiment was conducted under controlled mesocosm conditions to quantify the relative importance of sediment properties, topographic variation and warming for pioneer marsh vegetation performance.

Mytilus edulis and Magallana gigas growth rates from a three-month mesocosm experiment, Sylt, 2023

This dataset contains individual-level growth rate measurements of the blue mussel Mytilus edulis and the Pacific oyster Magallana gigas, collected during a controlled mesocosm experiment in Sylt, Germany (coordinates are included in the dataset). Data were collected over a three-month period, from 29 March 2023 to 26 June 2023. Twelve mesocosms were used: six maintained at ambient temperature and six maintained at +3 °C above ambient. A randomized experimental design was used: six mesocosms contained only M. edulis, while the remaining six contained both species. Observations include shell length and daily shell-length growth rates, total wet weight (including shell) and daily growth rates based on weight. Each mussel was labeled and categorized by size class. Measurements were obtained using digital calipers (The Noble Finn, Model 150 mm) and laboratory balances (Type 00AC, Sartorius AG Göttingen, Germany), as well as handheld multiparameter instruments for water temperature. The dataset provides reproducible, traceable individual-level responses of M. edulis and M. gigas under controlled warming, supporting studies on physiological responses of coastal bivalves to temperature changes.

Mytilus edulis growth rates from a three-month mesocosm experiment, Sylt, 2022

This dataset contains individual-level measurements of the blue mussel Mytilus edulis collected during a controlled mesocosm experiment at Sylt, Germany. Data were collected over a three-month period from 2 April 2022 to 27 June 2022. The experiment comprised twelve mesocosms, with four maintained at ambient temperature, four maintained at +1.5°C above ambient, and four at +3 °C above ambient. Observations include shell length, shell-length growth rates, and multiple biomass fractions, including total wet weight, wet tissue mass weight, shell-free dry weight, and ash-free dry weight, from which weight-based growth rates were calculated. All measurements were obtained using standard, traceable laboratory and field instruments. The dataset documents individual-level growth responses of M. edulis under controlled warming conditions and provides a reproducible resource for studies on physiological responses of coastal bivalves to temperature changes.

Mesocosm experiment on the influence of heatwave on plankton

In the context of global change, marine organisms are subjected not only to gradual changes in abiotic parameters, but also to an increasing number of extreme events, such as heatwaves. However, we still know little about the influence of heatwaves on the structure of marine communities, and experimental studies are needed to test the impact of heatwaves alone, and in combination with other environmental drivers. Here, we conducted a mesocosm experiment and applied an integrated multiple driver design to assess the potential impact of heatwaves under ambient and future environmental conditions on natural coastal plankton communities. To represent future environmental conditions, temperature and pH were manipulated based on the Representative Concentration Pathway 8.5 proposed by the IPCC for 2100, and dissolved N:P ratios were increased to simulate the conditions expected in European coastal zones. Throughout the experiment, we measured abiotic conditions as well as the abundance of bacterioplankton, phytoplankton, and microzooplankton.

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