Other language confidence: 0.7570500962368095
We studied dissolved organic matter (DOM) dynamics in the sea surface microlayer (SML) during a mesocosm study at the Sea sURface Facility (SURF) of the Institute for Chemistry and Biology of the Marine Environment (ICBM) in Wilhelmshaven, Germany (53.5148 °N, 8.1463 °E). The study was conducted from 18 May to 16 June 2023 as part of the multidisciplinary BASS research unit (Biogeochemical processes and Air-sea exchange in the Sea-Surface microlayer). SURF was filled with pretreated natural seawater from the nearby Jade Bay (53° 28' 42'' N, 8° 12' 15'' E) to replicate natural conditions. We selected this approach to examine the regrowth of surviving phytoplankton cells after the initial water treatments, simulating a native microbial community starting with almost no bioproduction or pre-existing bioproduction products. To induce and maintain the phytoplankton bloom, inorganic nitrogen, phosphorus, and silicate were added on May 26, May 31, and June 01, 2023. By that, we induced an algal bloom of Emiliania huxleyi and Cylindrotheca closterium. Water samples were collected using a glass plate for the SML and a tube at 40 cm depth for the underlying water (ULW). This dataset contains DOM molecular data from ultrahigh-resolution mass spectrometry (Fourier-transform ion cyclotron resonance mass spectrometer, FT-ICR-MS), molecular indices calculated from FT-ICR-MS data (Ibio, Iphoto, IDEG) and environmental data, including dissolved organic carbon (DOC) and dissolved organic nitrogen (DON). Furthermore, it contains attenuated total reflectance Fourier Transform Infrared (ATR-FTIR) data from representative samples for each bloom phase. By combining molecular analyses with nutrient and bloom-phase data, we highlight the in situ production of carbohydrate-like and laminarin-derived DOM as a significant contributor to SML composition. General metadata from the multidisciplinary mesocosm study, including temperature, salinity and chlorophyll a, are provided in Bibi et al. on PANGAEA at the following link: doi:10.1594/PANGAEA.984101.
The autonomous surface vehicle HALOBATES measured Essential Climate Variables (ECV), such as sea surface temperature (SST) and salinity (SSS), during the RV Heincke cruise HE614 in the harbor of Bremerhaven. HALOBATES captured the SST and SSS at six depths with a high vertical resolution of about 10 cm, from the near-surface layer (NSL) (between 30 and 100 cm) and the sea surface microlayer (SML) (upper millimeter). Conductivity, temperature, and depth (CTD) sensors measured temperature and conductivity (for salinity calculation) via a flow-through system on HALOBATES. Additional temperature sensors were mounted underneath the catamaran to measure in-situ temperature at six depths in the NSL. Salinity was corrected with discrete water samples to remove biases between the sensors. Two data loggers with several meteorological stations on the catamaran captured important weather variables during operation time. HALOBATES was operated on 14 and 15 March 2023.
During the RV Heincke cruise HE650 in the German Bight, the autonomous surface vehicle HALOBATES was deployed to obtain high-resolution measurements of key climate variables, including sea surface temperature (SST) and salinity (SSS). The platform sampled the upper ocean across five depths, extending from the near-surface layer (NSL; approximately 30–100 cm depth) to the sea-surface microlayer (SML), which occupies the uppermost millimetres of the ocean. Temperature and conductivity, from which salinity was derived, were measured using conductivity–temperature–depth (CTD) sensors integrated into HALOBATES' flow-through system. Additional temperature sensors mounted beneath the catamaran provided in situ observations at six NSL depths. Discrete water samples were used to calibrate the salinity measurements and account for potential sensor offsets. Meteorological conditions were simultaneously monitored using two data loggers equipped with meteorological stations installed on the catamaran. HALOBATES operated continuously during the deployment on 6th November 2024.
The file contains data from the Marine Carbon System. It gathered parameters from the inorganic carbon and incorporate the organic alkalinity as a main contributor to the sea surface microlayer (SML) compared to the Underlaying Water (ULW). Data was collected during Mesocosm Study from 18-May to 17-July 2024 in the Sea sURface Facility (SURF), Institute for Chemistry and Biology of the Marine Environment, Wilhelmshaven, Germany. Discrete samples to measure Dissolved Inorganic Carbon (DIC), Total Alkalinity (TA) and Organic Alkalinity (OA) were collected. For SML data, DIC, TA and OA was collected every third day following the glass‑plate technique (Harvey and Burzell, 1972). The ULW data, DIC, TA and OA were collected every day using a suction system to collect the sample from 0.4 m depth. Discrete samples were transported to the laboratory for further analysis; DIC was determined coulometrically (CM5017, UIC, IL, USA), and TA concentration was directly measured by high-precision closed-cell potentiometric titration (916 Ti-Touch, Metrohm, Switzerland). OA concentration was determined directly after TA was measured, using the same sample (from which all carbonate species had been purged), denoted as back titration. The dataset includes quality flags 0-4 with flags 1 and 2 are ready for use. See metadata for more information.
Here we present the dissolved organic matter (DOM) data of the sea surface microlayer (SML) and underlying water (ULW) during a multidisciplinary mesocosm study at the Sea sURface Facility (SURF) of the Institute for Chemistry and Biology of the Marine Environment (ICBM) in Wilhelmshaven, Germany (53.5148 °N, 8.1463 °E). The study was conducted from 18 May to 16 June 2023. Water samples were collected using a glass plate for the SML and a tube at a depth of 40 cm. DOM was extracted and desalinated by solid-phase extraction as described by Dittmar et al. (2008). The extracts were stored frozen in methanol until analysis. Aliquots were mixed with 50% ultrapure water (50:50 v/v) and diluted to a final carbon concentration of 2.5 ppm. DOM composition was analysed using a SolariX XR FT-ICR-MS (Bruker Daltonik GmbH, Bremen, Germany) with a 15 Tesla superconducting magnet and an electrospray ionisation source (ESI; Bruker Apollo II ion source) in negative ion mode. Data processing and molecular formula assignment were performed in ICBM-OCEAN, as described by Merder et al. (2020).
During the RV Heincke cruise HE626/2 in the German Bight, the autonomous surface vehicle HALOBATES was deployed to obtain high-resolution measurements of key climate variables, including sea surface temperature (SST) and salinity (SSS). The platform sampled the upper ocean across seven depths, with approximately 10 cm vertical spacing, extending from the near-surface layer (NSL; approximately 30–100 cm depth) to the sea-surface microlayer (SML), which occupies the uppermost millimetres of the ocean. Temperature and conductivity, from which salinity was derived, were measured using conductivity–temperature–depth (CTD) sensors integrated into HALOBATES' flow-through system. Additional temperature sensors mounted beneath the catamaran provided in situ observations at six NSL depths. Discrete water samples were used to calibrate the salinity measurements and account for potential sensor offsets. Meteorological conditions were simultaneously monitored using two data loggers equipped with meteorological stations installed on the catamaran. HALOBATES operated continuously during the deployment period from 27 October to 2 November 2023.
We studied dissolved organic matter (DOM) dynamics in the sea surface microlayer (SML) during a multidisciplinary mesocosm study at the Sea sURface Facility (SURF) of the Institute for Chemistry and Biology of the Marine Environment (ICBM) in Wilhelmshaven, Germany (53.5148 °N, 8.1463 °E). The study was conducted from 18 May to 16 June 2023 as part of the BASS research unit (Biogeochemical processes and Air-sea exchange in the Sea-Surface microlayer). This dataset contains environmental data, including dissolved organic carbon (DOC), dissolved organic nitrogen (DON) and DOM molecular indices (MLBwL, Ibio, Iphoto, IDEG) calculated from ultrahigh-resolution mass spectrometry data (Fourier-transform ion cyclotron resonance mass spectrometer, FT-ICR-MS). Furthermore, we present attenuated total reflectance Fourier Transform Infrared (ATR-FTIR) data from representative samples for each bloom phase. General metadata from the multidisciplinary mesocosm study, including temperature, salinity and chlorophyll a, are provided in Bibi et al. on PANGAEA at the following link: doi:10.1594/PANGAEA.984101.
The effects of a phytoplankton bloom and photobleaching on colored dissolved organic matter (CDOM) in the sea-surface microlayer (SML) and the underlying water (ULW) were studied in a month-long mesocosm study, in May and June of 2023, at the Institute for Chemistry and Biology of the Marine Environment (ICBM) in Wilhelmshaven, Germany. The mesocosm study was conducted by the DFG research group BASS (Biogeochemical processes and Air–sea exchange in the Sea-Surface microlayer, Bibi et al., 2025) in the Sea Surface Facility (SURF) of the ICBM. The facility contains an 8 m × 1.5 m × 0.8 m large outdoor basin with a retractable roof, which was closed at night and during rain events. The basin was filled with North Sea water from the adjacent Jade Bay. Homogeneity of the ULW in the basin was achieved by constant mixing of the water column. The daily SML and ULW samples were collected alternating in the morning, about 1 h after sunrise, and in the afternoon, about 10 h after sunrise. The alternation of sampling times intended to capture a potential effect of sun-exposure duration on DOM transformations and elucidated the day and night variability of the layers. The SML was collected via glass plate sampling (Cunliffe and Wurl, 2014). The ULW was sampled via a submerged tube and a connected syringe suction system in 0.4 m depth. The removed sample volume was refilled with Jade Bay water every day. SML and ULW samples were filtered through pre-flushed 0.7 µm Whatman GF/F and 0.2 nucleopore filters into clear 40 ml SUPELCO bottles. These bottles were acid-washed twice and combusted at 500 °C for 5 h. The samples were stored dark and at 4 °C and measured within a few days of the study. FDOM was measured using a Aqualog fluorescence spectrometer (Horiba Scientific, Japan) with 10 seconds integration time and high gain of the CCD (charge-coupled device) sensor within an excitation range from 240 to 500 nm, and an emission range from 209.15 to 618.53 nm. The Aqualog measures fluorescence as well as absorption. The resulting data includes an excitation-emission-matrix (EEM) of the blank (MilliQ Starna cuvette), an EEM of the sample, and the absorption values of the sample. The raw exported Aqualog data was corrected for errors and lamp shifts. The corrected EEM data is then decomposed by PARAFAC (Murphy et al., 2013) for its underlying fluorophore components. Before running the PARAFAC routine, the corrected data needed to undergo a correction process by subtracting the blank from the sample EEM and canceling the influences of the inner-filter effect (IFE, Parker & Rees, 1962; Kothawala et al., 2013). The fluorescence intensity of the IFE-corrected EEM is calibrated by using the Raman scatter peak of water (Lawaetz & Stedmon, 2009). For PARAFAC the corrected data was processed using the drEEM and NWAY toolbox (version 0.6.5; Murphy et al., 2013) in MATLAB (R2020b). A 4-component model was validated with the validation style S4C6T3 for the split half analysis with nonnegativity constraints and 1-8e as the convergence criteria with 50 random starts and a maximum number of 2500 iterations. The resulting final model had a core consistency of 88.11 and the explained percentage was 99.55%. Furthermore, four fluorescence indices were calculated from the corrected EEM data (HIX – Humification index, Zsolnay et al., 1999; BIX – Biological index, Huguet et al., 2009; REPIX – Recently produced index, Parlanti et al., 2000, Drozdowska et al., 2015; ARIX, Murphy, 2025).
Dissolved nitrous oxide (N₂O) concentrations were measured in the sea surface microlayer (SML, upper 1 mm) and the underlying water (ULW) during the BASS mesocosm study conducted with Jade Bay (southern North Sea) water from 16 May to 16 June 2023. The dataset contains N₂O concentrations from both water layers throughout the experimental period. Sampling of the SML was carried out using the glass plate method. The measurements cover conditions before, during and after a phytoplankton bloom.
The effects of a phytoplankton bloom and photobleaching on colored dissolved organic matter (CDOM) in the sea-surface microlayer (SML) and the underlying water (ULW) were studied in a month-long mesocosm study, in May and June of 2023, at the Institute for Chemistry and Biology of the Marine Environment (ICBM) in Wilhelmshaven, Germany. The mesocosm study was conducted by the DFG research group BASS (Biogeochemical processes and Air–sea exchange in the Sea-Surface microlayer, Bibi et al., 2025) in the Sea Surface Facility (SURF) of the ICBM. The facility contains an 8 m × 1.5 m × 0.8 m large outdoor basin with a retractable roof, which was closed at night and during rain events. The basin was filled with North Sea water from the adjacent Jade Bay. Homogeneity of the ULW in the basin was achieved by constant mixing of the water column. The daily SML and ULW samples were collected alternating in the morning, about 1 h after sunrise, and in the afternoon, about 10 h after sunrise. The alternation of sampling times intended to capture a potential effect of sun-exposure duration on DOM transformations and elucidated the day and night variability of the layers. The SML was collected via glass plate sampling (Cunliffe and Wurl, 2014). The ULW was sampled via a submerged tube and a connected syringe suction system in 0.4 m depth. The removed sample volume was refilled with Jade Bay water every day. SML and ULW samples were filtered through pre-flushed 0.7 µm Whatman GF/F and 0.2 nucleopore filters into brown bottles and were stored dark and at 4 °C until measurement within weeks of the study. The brown bottles were previously combusted at 500 °C. CDOM was measured with three liquid waveguide capillary cells (LWCC, WPI, USA) of different pathlengths (10 cm, 50 cm, 250 cm) to increase the measurement sensitivity following the protocols of Röttgers et al. (2024) using a spectral detector (Avantes, Netherlands) for a total spectral range from 230 to 750 nm. A sodium chloride (NaCl) solution was used for the salinity correction. The blank-corrected absorbance spectra were then converted into Napierian absorption coefficients (Bricaud et al., 1981).
| Organisation | Count |
|---|---|
| Bund | 2 |
| Wissenschaft | 51 |
| Type | Count |
|---|---|
| Daten und Messstellen | 51 |
| License | Count |
|---|---|
| Offen | 51 |
| Language | Count |
|---|---|
| Englisch | 51 |
| Resource type | Count |
|---|---|
| Archiv | 5 |
| Datei | 46 |
| Topic | Count |
|---|---|
| Boden | 18 |
| Lebewesen und Lebensräume | 51 |
| Luft | 16 |
| Mensch und Umwelt | 51 |
| Wasser | 51 |
| Weitere | 51 |