This dataset provides operational taxonomic units (OTU) abundance table of soil fungi with assigned taxonomic and functional guilds. The soil samples were collected in pure and beech-conifer mixed tree stands in Germany in November/December 2017, at 10 cm depth after removal of non-decomposed litter layer. The tree stands and site descriptions are provided (Ammer et al., 2020; Glatthorn, 2020). DNA was extracted from the samples using the DNeasy® PowerSoil® Pro kit (Qiagen, Hilden, Germany) and purified with DNeasy® PowerClean® kit (Qiagen, Hilden, Germany). Amplification (PCR) was achieved by using primers Mi_ITS3KYO2 (1:10) (Toju et al., 2012) and Mi_ITS4 (1:10) (White et al., 1990). The purification, pooling and quantification of PRC reactions were done as described (Clausing et al., 2020). Illumina sequencing was conducted by the Göttingen Genomics Laboratory (G2L) on MiSeq Platform using MiSeq Reagent Kit v3 (Illumina Inc., San Diego, USA). Raw paired - end reads (fasta.gz files: forward and reverse) were analyzed with MiSeq Marker gene pipeline V1.9. The abundance table contains 97% similarity fungal sequences rarefied to same number of counts (13668). Functional annotation of fungi was done using the FUNGuild database (Nguyen et al., 2016). The raw data were deposited in NCBI (National Center for Biotechnology Information) SRA (Sequence Read Archive) with bioproject accession number: PRJNA704813.
In the present project, we aim to study the very first processes of soil structuring to evaluate river restoration that plays a major role in environmental management and policy decisions, mainly due to increasing flood events. Agreement on what constitutes a successful restoration project continues to be lacking, and the relevant and novel approach proposed here will be the association of morphological and biological aspects of the soil structure. While the morphological approach (study of macro- and microstructure by the use of thin plates, soil aggregate stability measurements) takes into account the evolution of the soil in relation to the general climate, the biological one (fungal communities, earthworms, enchytraeids, organic matter) may help us to understanding soil organic matter incorporation. Moreover, we propose to validate these considerations by comparing them with the evolution of relatively undisturbed systems that may be considered as close approximations of conditions prior canalization.