Barley (Hordeum vulgare) is an important cereal grain which serves as major animal fodder crop as well as basis for malt beverages or staple food. Currently barley is ranked fourth in terms of quantity of cereal crops produced worldwide. In times of a constantly growing world population in conjunction with an unforeseeable climate change and groundwater depletion, the accumulation of knowledge concerning cereal growth and rate of yield gain is important. The Nordic Genetic Resource Center holds a major collection of barley mutants produced by irradiation or chemical treatment. One phenotypic group of barley varieties are dwarf mutants (erectoides, brachytic, semidwarf, uzu). They are characterized by a compact spike and high rate of yield while the straw is short and stiff, enhancing the lodging resistance of the plant. Obviously they are of applied interest, but they are also of scientific interest as virtually nothing is known about the genes behind the development of plant dwarfism. The aim of this project is to identify and isolate the genes carrying the mutations by using state of the art techniques for gene cloning at the Carlsberg Laboratory. The identified genes will be connected with the mutant phenotype to reveal the gene function in general. One or two genes will be overexpressed and the resulting recombinant proteins will be biochemically and structurally characterized. The insights how the mutation effects the protein will display the protein function in particular. Identified genes and their mutant alleles will be tested in the barley breeding program of the Carlsberg brewery.
Salinity occurs often simultaneously with drought stress. Therefore, breeding for tolerance to combined both stresses can contribute significantly to crop yield. However, classical selection in salinity has generally been unsuccessful, partly due to high variability of salt stress resulting from the different salinity and drought status. Unfortunately, the use of unrealistic stress protocols for mimicking salinity and drought stress is the norm rather than the exception in biotechnological studies. Therefore, the great challenge is to gain knowledge required to develop plants with enhanced tolerance to field conditions. Our overall hypothesis is that a realistic stress protocol simulating a field environment with combined salt and drought stress as a platform for precision phenotyping of plant tolerance to salinity may solve this problem. This study will demonstrate that highly managed stress environments can be created and key traits of plants can be characterised by using advanced non-destructive sensors that are able to identify relevant traits of plants.
Eine hohe Resistenz gegen Bodenpathogene, gute Standortanpassung und Veredlungsaffinität sind die entscheidenden Merkmale von Unterlagen. Bei der Pathogenresistenz ist bei Reben die Widerstandsfähigkeit gegen die Reblaus Daktulosphaira vitifoiae essentiell, da die europäische Kulturrebe Vitis vinifera L über keinerlei Resistenzen verfügt und nur an wenigen Standorten ein wurzelechter Anbau möglich ist. Amerikanische Wildformen mit solchen Reblausresistenzen sind daher in der Unterlagenzüchtung von großer Bedeutung. Die langfristige Sicherung solcher Genotypen ist daher eine Voraussetzung für spätere Züchtungsarbeiten zur Erstellung neuer besserer Unterlagen. Daneben spielt auch die Standortanpassung eine wichtige Rolle. Vitis berlandieri ist hier am wichtigsten, da sie als einzige Art über eine hohe Kalkverträglichkeit verfügt und die Mehrheit der deutschen und europäischen Weinbaustandorte durch hohe Kalkgehalte im Boden charakterisiert sind. Kalkempfindliche Arten leiden unter Kalkchlorose mit stark vermindertem Wuchs. Aufgrund begrenzter Verfügbarkeit wurden jedoch nur wenige Pflanzen der Art in der Unterlagenzüchtung verwendet und damit nur ein Teil des Potentials der Art genutzt. In einem gemeinsamen Projekt mit dem United States Department for Agriculture wurden daher im September 2005 im ursprünglichen Verbreitungsgebiet der Art in Zentraltexas Samen von Wildformen gesammelt und die Hälfte davon in Geisenheim zur Keimung gebracht und ausgepflanzt. Derzeit werden mehr als 5000 Pflanzen in der in vivo Erhaltung. In den kommenden Jahren werden diese hinsichtlich ihrer relevanten Eigenschaften phänotypisch charakterisiert und in einem späteren Stadium auch genotypisiert, um für weitere Kreuzungs- und Selektionsarbeiten nutzbares material zu identifizieren.
Eine hohe Resistenz gegen Bodenpathogene, gute Standortanpassung und Veredlungsaffinität sind die entscheidenden Merkmale von Unterlagen. Bei der Pathogenresistenz ist bei Reben die Widerstandsfähigkeit gegen die Reblaus Daktulosphaira vitifoiae essentiell, da die europäische Kulturrebe Vitis vinifera L über keinerlei Resistenzen verfügt und nur an wenigen Standorten ein wurzelechter Anbau möglich ist. Klimaveränderungen erfordern neue Unterlagen mit hoher Reblausfestigkeit und besserer Standortanpassung. Aufgrund der derzeitigen Szenarien werden sowohl Trockenresistenz als auch Toleranz gegen hohe Kalkgehalte insbesondere in Verbindung mit hohem Bodenwassergehalte zukünftig von Bedeutung sein. Hierfür werden entsprechende Kreuzungen vorgenommen, die Sämlinge aufgezogen, auf ihre Reblausfestigkeit getestet und anschießend Prüfungen der Wurzelungs- und Veredlungsfähigkeit vorgenommen. Anschließend wird die Witterungs- und Bodenanpassung der Zuchtstämme insbesondere auf Trocken- und Kalkstandorten untersucht. Ziel ist die Entwicklung verschiedener Unteralgen, die eine vollständige Reblausresistenz mit hohen Trockenheits- und/oder Kalktoleranz kombinieren.
Abiotic environmental stresses are among the major factors limiting agricultural productivity in many developing countries. A common feature of various environmental stresses is the excessive accumulation of reactive oxygen species (ROS) in the leaf tissue leading to 'oxidative stress' and in turn visible leaf lesions, reduced growth, and in severe cases plant death. This project aims at identifying molecular mechanisms associated with oxidative stress tolerance in rice (Oryza sativa L.) under three different environmental conditions: (i) high tropospheric ozone concentration, (ii) zinc deficiency, and (iii) iron toxicity. This is achieved by dissecting naturally occurring genotypic variability in oxidative stress tolerance into distinct quantitative trait loci (QTL). Physiological mechanisms and genes underlying such tolerance QTL are identified by adopting an interdisciplinary approach including biochemical characterization of the antioxidant systems, transcriptome profiling, and experiments with gene knock-out mutants for candidate genes. Theoretical understanding of stress tolerance mechanisms obtained from laboratory experiments would be validated in field experiments together with international research institutions and partners in developing countries. At a later stage, the project strives to adopt emerging techniques in gene discovery such as single nucleotide polymorphism (SNP) based association mapping, and apply lessons learned from studying the 'model cereal crop' rice to other species such as barley (Hordeum vulgare L.). The project is expected to contribute to world-wide efforts in adapting crop production to stress environments by specifically advancing the understanding of oxidative stress tolerance.
In the second phase of subproject 3.1 is envisaged to model representative farms. The models will be structured in a way that not only the production but also the material balances can be represented. In particular the nitrogen balance, the carbon balance and the water balance will be incorporated. Gas and particulate matter emissions can be considered as well, as far as there are sufficient data available. The modelling aims at integrating on farm level all baseline data surveyed in other sub-projects and at quantifying and valuating at farm level the already worked out management measures for reducing environmental burden while increasing production efficiency. The focus will be on measures for reducing nitrogen fertilisation, the interrelation between management activities and carbon/nitrogen balance, effects of nitrogen deposition on the operational nutrient management, water efficiency, etc. Another part for the impact analysis shall be the extent to which cropping practices elaborated in other sub-projects can be alternatives for the prevailing wheat-maize-rotation. Thereby cotton, groundnuts, soybeans, vegetables, and row intercropping are to be analysed and valued on farm level. Furthermore, the sub-project shall provide other sub-projects with indicators which are necessary in order to optimise breeding and management programmes under consideration of economic factors. Another aim of the sub-project is to carry out economic impact analyses of possible policy measures. To do so, realistic concepts of the Chinese agricultural policy as e.g. a reduction of the subsidies on means of production, in particular on nitrogen, water, etc. and their possible impact on quantities produced, input quantities applied, nutrient and water efficiency, income and other criteria are to be investigated. Furthermore, the influence of stages of an increasing mechanisation on production, income and structural changes can be investigated. Finally, open questions and approaches for further research will be derived through a sensitivity analysis.
Die Entwicklung von Sorten, die bei einer effizienteren Nutzung vorhandenen Stickstoffs hohe Ertragsstabilität und hohe Backqualität auch unter ungünstigen Umweltbedingungen garantieren, ist eine Herausforderung in Hinblick auf das erhöhte Risiko für das Auftreten von Hitzewellen und Trockenheit in ausgedehnten Gebieten Zentral- und Osteuropas. EFFICIENT WHEAT ist ein kollektives Forschungsprojekt der Österreichischen Agentur für Gesundheit und Ernährungssicherheit (AGES), der TU München Lehrstuhl Pflanzenzüchtung (TUM), der Bayerischen Landesanstalt für Landwirtschaft (LfL), KWS Lochow GmbH, Lantmännen SW Seed GmbH, Saatzucht Streng-Engelen GmbH & Co. KG, Secobra Saatzucht GmbH, Saatzucht Donau GesmbH & Co.KG und Saatzucht Edelhof. Da die ungarische Agrarwirtschaft ganz besonders stark vom Problem Trockenheit betroffen ist, besteht außerdem eine Zusammenarbeit mit der Cereal Research Non-profit Company (CRC). Angestrebt wird die Beschleunigung des Züchtungsfortschritts bei Winterweizen durch eine Verbesserung des vorhandenen Zuchtmaterials hinsichtlich Stickstoffnutzungseffizienz (NUE) und Trockentoleranz ohne signifikante Ertrags- oder Qualitätseinbußen. Dabei werden im Einzelnen die Identifizierung von Sorten mit hohem Ertrags- und Qualitätspotential, welche Gene tragen, die mit einer höheren NUE unter Trockenstress assoziiert sind, die Etablierung von Screening-Techniken für N-assoziierte Merkmale zur großflächigen Anwendung im Zuchtgarten sowie die Entwicklung molekularer Marker für die markergestützte Selektion anvisiert. Drei verschiedene Arbeitspakete werden bearbeitet: 1. die phänotypische Charakterisierung von 30 europäischen Elitewinterweizengenotypen in Feldversuchen an 11 (klimatisch) unterschiedlichen Standorten in Deutschland, Österreich und Ungarn, 2. die Analyse indirekter und direkter Backqualitätsparameter sowie 3. Assoziationskartierung von QTL für eine verbesserte NUE unter Trockenstressbedingungen. Im Rahmen von EFFICIENT WHEAT soll geprüft werden, ob die vorhandenen europäischen Hochertragssorten wertvolle Allele für eine verbesserte Stickstoffnutzungseffizienz tragen, die unter Trockenstress wirksam werden. Das Kernsortiment von 30 Winterweizengenotypen, gezüchtet in Österreich, Deutschland, Ungarn, Frankreich und England, stellt das Elitezuchtmaterial unterschiedlicher klimatischer Regionen dar. Diese ausgewählten Sorten werden im Feld bei natürlicher Bewässerung und auf sandigen Böden mit zusätzlicher Bewässerung angebaut. Weiterhin werden Gewächshausversuche unter kontrollierten Bedingungen durchgeführt. Als Grundlage für die angestrebte Marker-Merkmals-Assoziationskartierung werden morpho-physiologische Merkmale erfasst, die allelische Variation von N-assoziierten Kandidatengenen innerhalb der Winterweizensorten untersucht sowie stickstoffrelevante Parameter in Stroh, Korn und Boden ermittelt. usw.
The fire blight disease caused by the bacterium Erwinia amylovora, is currently the major constraint for apple production in several European countries including Germany and Switzerland. In recent years several infections occurred which have led to costs in the tens of millions Euros. The antibiotic Streptomycin (Plantomycin®), which is the only effective plant protective agent, can only be applied under particular restrictions. Planting of fire blight resistant cultivars seems to be the most promising solution, which is environmental and producer friendly. Unfortunately, the available fire blight resistant apple cultivars are non-competitive on the market, because of their inferior fruit quality. The improvement of established cultivars like 'Golden Delicious' or 'Gala' using classical breeding strategies is impossible because apple is self-incompatible and heterozygous. Each offspring of a sexual cross between such a cultivar and a resistant genotype will be genetically different to each of the parents. Linkage drag coming from the resistant led often to a decrease in fruit quality. Several crosses with cultivars of good quality are necessary to remove most of the unwanted traits and the result will be a completely new cultivar. Breeding of such a new apple cultivar is time consuming and in the end it is not clear, whether the new cultivar will be accepted by consumers and growers or not. Under these premises, the introduction of a specific gene into a particular cultivar which already has all qualities necessary, except the trait in question, is attractive. The recombinant DNA-technology offers the most promising strategy to solve the problem of fire blight by using Malus own genes without the need to change the popular cultivars. The development of cisgenic plants seems to be a realistic and successful strategy. Cisgenic plants contain only genes, which originates from crossable species and not from outside of the primary genepool. They are therefore comparable to classical bred plants and expected to be acceptable by consumers and growers. The methodology to produce such a durable resistant cisgenic apple cultivar has already been established, but an apple fire blight resistance gene (or linked gene set) is still needed. Recently a strong Fieblight resistance has been identified on chromosome 3 of the apple hybrid Malus x robusta 5. This project focuses on the isolation of the gene(s) inducing the resistance of M. x robusta 5 and its cloning into the susceptible cultivar Gala. Furthermore the interaction of this (or these) genes products with gene products of the pathogen will be studied so to understand the interaction mechanisms and therefore evaluate the durability of the resistance.
Do genetically modified strawberries pose a threat to wild varieties? Do genetically modified strawberries pose a threat to wild varieties? Strawberries are an important niche product in Switzerland. Breeders are experimenting with genetic engineering methods to enhance the marketability of this product. There are risks inherent in this approach since the transfer of modified genes to wild strawberries could endanger the continued existence of the wild varieties. Background Transgenic varieties of strawberry with higher yields and enhanced root development already exist. The first release trials have already begun in Italy. However, if transgenic varieties of strawberry cross-breed with wild ones, there may be negative effects. The hybrids produced in this way are often sterile, yet by back-crossing with wild types or by producing prolific numbers of offshoots they can penetrate the native flora and displace it. Objectives This project has two basic goals. First, it seeks to assess the extent to which transgenic strawberries are capable of cross-breeding with their wild relatives. Second, it seeks to investigate the possible ecological impact of such crossbreeding under various environmental conditions in order to assess the risks associated with cultivating transgenic strawberries in the open. Methods Greenhouse experiments with honey bees, the most important pollinators of strawberries, will be carried out to show whether and how efficiently natural pollination occurs between transgenic and wild strawberries. Genetic methods will be used to determine how frequently foreign pollination between cultivated and wild strawberries has already occurred in the open in the past. The possible ecological implications of this will be quantified using life history data such as growth and competitive pressure. In these experiments, transgenic and artificially cross-bred strawberries from the laboratory will be planted in various soils. Significance The cultivation of transgenic plants is associated with potential risks for their wild relatives. Scientists have warned that the latter could become extinct as a result of undesirable cross-breeding. However, to date the true extent of these risks has barely been investigated. This project aims to close this gap by generating basic data with transgenic and wild strawberries as model organisms. These data could ultimately be relevant for other related crop plants such as apple trees or cherry trees.
The proposed RTN adopts a unified approach to a unique sainfoin (Onobrychis viciifolia) collection. It will provide excellent training for young researchers in evaluating traditional resources and developing novel strategies for sustainable agriculture. This is timely because of the pending CAP reforms. The RTN will offer multisectorial and multidisciplinary training at 12 first-class research institutions and 3 SMEs in 11 countries. It is based on a structured combination of research and training activities to ensure that the young researchers will achieve optimal development of professional skills for their future careers . The young researchers will greatly benefit from the vast expertise in a wide range of disciplines amongst the partners: agronomy, plant breeding, seed production and marketing, animal nutrition, veterinary science, chemical analysis, biochemistry, genetics and molecular biology. The scientific approach will develop a scientific and technical basis for animal feeding systems based on lower chemical inputs by re-popularising traditional fodder legumes for more efficient, animal- and environment-friendly farming systems. Sainfoin will be a showcase for an excellent fodder legume, which was widely grown in Europe before the use of commercial fertilisers and synthetic drugs. Currently, a considerable amount of research occurs on sainfoin but includes only a few cultivars. This prevents exploitation of its full genetic potential. The unique collection already available within this network and a concerted evaluation will lay the foundation for exploiting the full potential of this traditional forage crop in contemporary cultivation systems. Training will consist of extensive scientific education on a local and network-wide basis and include complementary skills, e.g. foreign languages, personal, social and inter-cultural skills (management skills and soft skills). The proposed RTN adopts a unified approach to a unique sainfoin (Onobrychis viciifolia) collection. It will provide excellent training for young researchers in evaluating traditional resources and developing novel strategies for sustainable agriculture. This is timely because of the pending CAP reforms. The RTN will offer multisectorial and multidisciplinary training at 12 first-class research institutions and 3 SMEs in 11 countries. It is based on a structured combination of research and training activities to ensure that the young researchers will achieve optimal development of professional skills for their future careers . The young researchers will greatly benefit from the vast expertise in a wide range of disciplines amongst the partners: agronomy, plant breeding, seed production and marketing, animal nutrition, veterinary science, chemical analysis, biochemistry, genetics and molecular biology...
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