Barley (Hordeum vulgare) is an important cereal grain which serves as major animal fodder crop as well as basis for malt beverages or staple food. Currently barley is ranked fourth in terms of quantity of cereal crops produced worldwide. In times of a constantly growing world population in conjunction with an unforeseeable climate change and groundwater depletion, the accumulation of knowledge concerning cereal growth and rate of yield gain is important. The Nordic Genetic Resource Center holds a major collection of barley mutants produced by irradiation or chemical treatment. One phenotypic group of barley varieties are dwarf mutants (erectoides, brachytic, semidwarf, uzu). They are characterized by a compact spike and high rate of yield while the straw is short and stiff, enhancing the lodging resistance of the plant. Obviously they are of applied interest, but they are also of scientific interest as virtually nothing is known about the genes behind the development of plant dwarfism. The aim of this project is to identify and isolate the genes carrying the mutations by using state of the art techniques for gene cloning at the Carlsberg Laboratory. The identified genes will be connected with the mutant phenotype to reveal the gene function in general. One or two genes will be overexpressed and the resulting recombinant proteins will be biochemically and structurally characterized. The insights how the mutation effects the protein will display the protein function in particular. Identified genes and their mutant alleles will be tested in the barley breeding program of the Carlsberg brewery.
Salinity occurs often simultaneously with drought stress. Therefore, breeding for tolerance to combined both stresses can contribute significantly to crop yield. However, classical selection in salinity has generally been unsuccessful, partly due to high variability of salt stress resulting from the different salinity and drought status. Unfortunately, the use of unrealistic stress protocols for mimicking salinity and drought stress is the norm rather than the exception in biotechnological studies. Therefore, the great challenge is to gain knowledge required to develop plants with enhanced tolerance to field conditions. Our overall hypothesis is that a realistic stress protocol simulating a field environment with combined salt and drought stress as a platform for precision phenotyping of plant tolerance to salinity may solve this problem. This study will demonstrate that highly managed stress environments can be created and key traits of plants can be characterised by using advanced non-destructive sensors that are able to identify relevant traits of plants.
Eine hohe Resistenz gegen Bodenpathogene, gute Standortanpassung und Veredlungsaffinität sind die entscheidenden Merkmale von Unterlagen. Bei der Pathogenresistenz ist bei Reben die Widerstandsfähigkeit gegen die Reblaus Daktulosphaira vitifoiae essentiell, da die europäische Kulturrebe Vitis vinifera L über keinerlei Resistenzen verfügt und nur an wenigen Standorten ein wurzelechter Anbau möglich ist. Amerikanische Wildformen mit solchen Reblausresistenzen sind daher in der Unterlagenzüchtung von großer Bedeutung. Die langfristige Sicherung solcher Genotypen ist daher eine Voraussetzung für spätere Züchtungsarbeiten zur Erstellung neuer besserer Unterlagen. Daneben spielt auch die Standortanpassung eine wichtige Rolle. Vitis berlandieri ist hier am wichtigsten, da sie als einzige Art über eine hohe Kalkverträglichkeit verfügt und die Mehrheit der deutschen und europäischen Weinbaustandorte durch hohe Kalkgehalte im Boden charakterisiert sind. Kalkempfindliche Arten leiden unter Kalkchlorose mit stark vermindertem Wuchs. Aufgrund begrenzter Verfügbarkeit wurden jedoch nur wenige Pflanzen der Art in der Unterlagenzüchtung verwendet und damit nur ein Teil des Potentials der Art genutzt. In einem gemeinsamen Projekt mit dem United States Department for Agriculture wurden daher im September 2005 im ursprünglichen Verbreitungsgebiet der Art in Zentraltexas Samen von Wildformen gesammelt und die Hälfte davon in Geisenheim zur Keimung gebracht und ausgepflanzt. Derzeit werden mehr als 5000 Pflanzen in der in vivo Erhaltung. In den kommenden Jahren werden diese hinsichtlich ihrer relevanten Eigenschaften phänotypisch charakterisiert und in einem späteren Stadium auch genotypisiert, um für weitere Kreuzungs- und Selektionsarbeiten nutzbares material zu identifizieren.
Eine hohe Resistenz gegen Bodenpathogene, gute Standortanpassung und Veredlungsaffinität sind die entscheidenden Merkmale von Unterlagen. Bei der Pathogenresistenz ist bei Reben die Widerstandsfähigkeit gegen die Reblaus Daktulosphaira vitifoiae essentiell, da die europäische Kulturrebe Vitis vinifera L über keinerlei Resistenzen verfügt und nur an wenigen Standorten ein wurzelechter Anbau möglich ist. Klimaveränderungen erfordern neue Unterlagen mit hoher Reblausfestigkeit und besserer Standortanpassung. Aufgrund der derzeitigen Szenarien werden sowohl Trockenresistenz als auch Toleranz gegen hohe Kalkgehalte insbesondere in Verbindung mit hohem Bodenwassergehalte zukünftig von Bedeutung sein. Hierfür werden entsprechende Kreuzungen vorgenommen, die Sämlinge aufgezogen, auf ihre Reblausfestigkeit getestet und anschießend Prüfungen der Wurzelungs- und Veredlungsfähigkeit vorgenommen. Anschließend wird die Witterungs- und Bodenanpassung der Zuchtstämme insbesondere auf Trocken- und Kalkstandorten untersucht. Ziel ist die Entwicklung verschiedener Unteralgen, die eine vollständige Reblausresistenz mit hohen Trockenheits- und/oder Kalktoleranz kombinieren.
Die Flussperlmuschel (Margaritifera margaritifera) ist als vom Aussterben bedrohte Art einzustufen. Die Nachzucht der Muschel gelang in den letzten Jahren mittels einer eigens errichteten Nachzuchtstation jedoch durchaus sehr erfolgreich. Um für die Wiederansiedelung der Flussperlmuschel in Fließgewässern zu gewährleisten gilt es vorab geeignete Lebensraumbedingungen zu finden. Dabei ist die Habitatmodellierung als geeignetes Instrument für eine Bewertung bzw. eine Vorauswahl anzusehen. Diese Modellierung beruht auf Eingangsparametern, die in intakten Flussperlmuschelgewässern erhoben werden müssen. Solche intakten Gewässer mit reproduktiven Flussperlmuschelpopulationen existieren aktuell nur noch in Nordeuropa (z. B. Schweden, Finnland, Norwegen) und in einem deutschen Heidebach, der Lutter, in der ein umfangreiches, das gesamte Einzugsgebiet umfassendes Sanierungs- und Wiederansiedelungsprojekt sehr erfolgreich umgesetzt wurde. Ziel des Projektes ist es, in verschiedenen österreichischen Gewässern jene (Lebensraum-)Parameter zu erfassen, welche von der Flussperlmuschel genutzt bzw. auch nicht genutzt werden, um funktionale Zusammenhänge für ein zukünftiges Management zu erkennen, bzw. auch jene Nutzungskurven abzuleiten, die für mögliche hydraulische Modellanwendungen zur Habitatevaluierung benötigt werden. Um dieses Ziel zu Erreichen wird eine Charakterisierung der Gewässermorphologie mittels tachymetrischer Vermessung von genutzten und nicht genutzten Gewässerprofilen durchgeführt als Grundlage für die eindimensionale numerische Modellierung (HEC-RAS). Weiters werden Kornverteilungskurven mittels volumetrischer Beprobung aus Deck- und Unterschicht erstellt mit einer Bestimmung der Feinsedimentanteile (kleiner als 0,063 mm, kleiner als 0,125 mm, kleiner als 0,250 mm) durchgeführt.
The impacts of global climate change will also affect the winter wheat production in Central Europe. Cultivars with improved yield stability and high quality when exposed to abiotic stresses like drought and heat are necessary to continue the leading position of the European Union at the global wheat market. In general breeding towards improved yield stability has been hindered by its quantitative genetic basis and the complexity of abiotic stress tolerance mechanisms and up till now was focused in permanent dryland conditions using spring wheat cultivars. The strategy proposed for the current study is to exploit high yielding European breeding material for drought/heat tolerance with no or limited negative genetic linkage to yield components. For the characterization of morpho-physiological attributes visual screening, spectral reflectance as well as in plant biochemical measurements will be performed together with molecular genetic analyses of important genomic regions and candidate genes. This project will result in the identification of valuable screening tools and molecular markers that will help the breeders to speed up the development of new cultivars with improved yield stability and high quality under environmental stress. The SME dominated plant breeding and seed branch in Germany, Austria and Hungary will be the beneficiaries of WHEAT STRESS. Project results will contribute to maintain their competitiveness by new breeding techniques and tools, and identification of quality parameters. One third of the transnational SME-User Committee is directly involved in project activities (field trials and data collection) and interacts closely with the scientists. This guarantees that project results will gain direct and broad access into the practical work of the breeders. Furthermore WHEAT STRESS affects societal needs: security of food/feed, preservation of water resources, and stability of rural environments and agricultural employment.
Do genetically modified strawberries pose a threat to wild varieties? Do genetically modified strawberries pose a threat to wild varieties? Strawberries are an important niche product in Switzerland. Breeders are experimenting with genetic engineering methods to enhance the marketability of this product. There are risks inherent in this approach since the transfer of modified genes to wild strawberries could endanger the continued existence of the wild varieties. Background Transgenic varieties of strawberry with higher yields and enhanced root development already exist. The first release trials have already begun in Italy. However, if transgenic varieties of strawberry cross-breed with wild ones, there may be negative effects. The hybrids produced in this way are often sterile, yet by back-crossing with wild types or by producing prolific numbers of offshoots they can penetrate the native flora and displace it. Objectives This project has two basic goals. First, it seeks to assess the extent to which transgenic strawberries are capable of cross-breeding with their wild relatives. Second, it seeks to investigate the possible ecological impact of such crossbreeding under various environmental conditions in order to assess the risks associated with cultivating transgenic strawberries in the open. Methods Greenhouse experiments with honey bees, the most important pollinators of strawberries, will be carried out to show whether and how efficiently natural pollination occurs between transgenic and wild strawberries. Genetic methods will be used to determine how frequently foreign pollination between cultivated and wild strawberries has already occurred in the open in the past. The possible ecological implications of this will be quantified using life history data such as growth and competitive pressure. In these experiments, transgenic and artificially cross-bred strawberries from the laboratory will be planted in various soils. Significance The cultivation of transgenic plants is associated with potential risks for their wild relatives. Scientists have warned that the latter could become extinct as a result of undesirable cross-breeding. However, to date the true extent of these risks has barely been investigated. This project aims to close this gap by generating basic data with transgenic and wild strawberries as model organisms. These data could ultimately be relevant for other related crop plants such as apple trees or cherry trees.
The background of the presented research effort is the fact that on one hand increased biomass production is required not only for alimentation purposes but also for the substitution of fossil and nuclear fuels and PVC plastics, and that on the other hand soil is scarce and increased bioenergy production should not reduce food and feedstock production and has to be sustainable, preserving soil and contributing to climate change mitigation. The project consortium has identified unused potentials within the crop rotation schemes and in perennial grassrland in many European regions. A quantitative analysis of unused crop potentials stemming from incomplete crop rotation in Europe as well as of unused yield potentials of perennial grasslands will be the first step. The analysis will be based on CORINE data and will cover all Euopean agricultural regions. Subsequently, identified hot spots with high potential for additional crops will be mapped. Additional crops will be taken into account to the extent that they can be produced without reducing existing food and feedstock crops, if they can be produced without ecologically harmful additional irrigation and mineral fertilization measures, and if they allow also sufficient economic yields. Such additional crops will be seen as potential raw material for bioenergy production. In most cases, these crops will be species mixtures with short cultivation periods (2-4 months within the vegetation period plus eventually winter). If used for biogas production or green bio-refinery proceeding, economically feasible and ecologically sound production will require de-centralized bio-energy production including the use of digestate for soil fertilization. Existing bioenergy plants fed with maize silage can be supplied by cover crop silage, instead, thus allowing additional food production and unbundling of existing food-fuel competition. Consequences of such altered land-use solutions on nutrient circulation and on climate shall be assessed in a special work-package. One work package will be devoted to practical demonstration and result validation. Three case-studies will be undertaken - one in alpine or boreal grassland, one in the corn cultivation belt for cover crops following wheat or rye in fall, and one for mediterranean agricultural systems. Demonstration sites will be selected, land management costs and yields per hectare will be monitored, nitrogen balances will be set up. Consequences of all-year soil coverage on soil will be assessed in these demo areas for different crop mixtures cultivated in different ways (different timing, sowing, harvesting and fertilizing). Capitalization of research findings will build on a mixture of publication activities in referenced and expert journals, in conference presentations and networking activities. The European seed production research network EUCARPIA will play an active role in this task. The novel aspect is the targeted use of catch crops and cover crops for energy
The proposed RTN adopts a unified approach to a unique sainfoin (Onobrychis viciifolia) collection. It will provide excellent training for young researchers in evaluating traditional resources and developing novel strategies for sustainable agriculture. This is timely because of the pending CAP reforms. The RTN will offer multisectorial and multidisciplinary training at 12 first-class research institutions and 3 SMEs in 11 countries. It is based on a structured combination of research and training activities to ensure that the young researchers will achieve optimal development of professional skills for their future careers . The young researchers will greatly benefit from the vast expertise in a wide range of disciplines amongst the partners: agronomy, plant breeding, seed production and marketing, animal nutrition, veterinary science, chemical analysis, biochemistry, genetics and molecular biology. The scientific approach will develop a scientific and technical basis for animal feeding systems based on lower chemical inputs by re-popularising traditional fodder legumes for more efficient, animal- and environment-friendly farming systems. Sainfoin will be a showcase for an excellent fodder legume, which was widely grown in Europe before the use of commercial fertilisers and synthetic drugs. Currently, a considerable amount of research occurs on sainfoin but includes only a few cultivars. This prevents exploitation of its full genetic potential. The unique collection already available within this network and a concerted evaluation will lay the foundation for exploiting the full potential of this traditional forage crop in contemporary cultivation systems. Training will consist of extensive scientific education on a local and network-wide basis and include complementary skills, e.g. foreign languages, personal, social and inter-cultural skills (management skills and soft skills). The proposed RTN adopts a unified approach to a unique sainfoin (Onobrychis viciifolia) collection. It will provide excellent training for young researchers in evaluating traditional resources and developing novel strategies for sustainable agriculture. This is timely because of the pending CAP reforms. The RTN will offer multisectorial and multidisciplinary training at 12 first-class research institutions and 3 SMEs in 11 countries. It is based on a structured combination of research and training activities to ensure that the young researchers will achieve optimal development of professional skills for their future careers . The young researchers will greatly benefit from the vast expertise in a wide range of disciplines amongst the partners: agronomy, plant breeding, seed production and marketing, animal nutrition, veterinary science, chemical analysis, biochemistry, genetics and molecular biology...
Honey is among the oldest food products of mankind and beekeeping is deeply rooted in every European culture. Numerous European and national regulations control honey quality, which reflects both the high nutritional and societal value of the product. Yet in an environment with increasing chemical pollution and the wide use of agrochemicals, honey runs high risks of becoming chemically polluted. In addition a broad spectrum of chemicals is used to treat honeybee diseases, further contaminating honey with sometimes highly toxic compounds. The BEE SHOP is a network of ten leading European honeybee research groups in honey quality, pathology, genetics and behaviour as well as selected beekeeping industries, which all share a common interest in promoting Europe's high honey quality standards. The prime goal is to reduce potential sources of honey contamination due to both foraging contaminated nectar and chemotherapy of honeybee diseases. The BEE SHOP will therefore deal with the development of biological resistance to pests and pathogens to avoid chemotherapy. Various European honeybee races and populations will be screened for their disease resistance potential to the main pressing pathogens. Differences in foraging patterns among European honeybees and their underlying mechanisms will be studied to identify behavioural traits reducing contamination. Differences in disease susceptibility will be genetically analysed by QTL mapping. Major loci in the genome will be identified with the aid of the published honeybee genome. SNPs will be developed to allow for selection of specific target genes in both drones and queens before insemination. This will greatly accelerate the selection progress in honeybee breeding allowing for the swift establishment of resistant but efficient stock. New tools for testing honey quality and authenticity will be developed to allow inspections of honey according to the current EC directives on honey quality and organic beekeeping.
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