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Found 2636 results.

Measured environmental concentrations of copper and hazard quotients in the German Bight in summer from 1986 to 2021

Data includes the measured environmental concentrations (MEC) of the summer copper (Cu) concentration in the German Bight from 1986 to 2021 (MUDAB database, https://geoportal.bafg.de/MUDABAnwendung/), including sampling points coordinates, year of sampling and Cu concentration. Additionally the Hazard quotient (HQ) is provided by dividing the MEC with the predicted no effect concentration (PNEC), defined as EC10 estimates from Crassostrea gigas embryos exposed for 48 h at 18°C and LC10 estimates from C. gigas larvae exposed for 24 h at 24°C, divided by an assessment factor (AF) of 5.

Benthic biogeochemistry data from RV HEINCKE cruise HE582 to the German Bight, North Sea in late summer 2021

This dataset contains data from the RV Heincke cruise HE582 to the German Bight of the North Sea in late summer 2021. The aim of the research was to investigate the source of sedimentary glycan concentrations in subtidal sandy sediments. Glycans represent a substantial fraction of extracellular polymeric substances and may affect flow dynamics in marine sandy sediments. The origin and concentration of glycans in sands remain understudied until today. To gain insights into oxygen supply and glycan concentrations in sandy sediment, we conducted in situ measurements and sampled sediment via a van Veen grab for ex situ investigations. Oxygen penetration depths were determined by a benthic lander, which was deployed for ca. 24h at each station. Chlorophyll a concentrations as an indicator for potentially photosynthetically active sedimentary biomass were derived via extraction with 90% acetone against Sigma Aldrich standards. Glycan concentrations served as indicator for extracellular polymeric substances and were quantified against a glucose standard curve via a phenol sulfuric acid assay after prior sequential glycan extraction (MilliQ, EDTA, NaOH). The final glycan concentrations are referred to per volume of porespace, and therefore given in mmol/l porewater. To investigate if benthic primary producers could be responsible for the extracted sedimentary glycan concentrations, we conducted stable isotope incubations.

Total carbohydrates quantified in sediment cores from coastal vegetated ecosystems

50-cm deep sediment cores were taken in saltmarsh, seagrass, mangroves and unvegetated areas around the German Bight, Malaysia and Columbia in 2022 and 2023. Up to 3 points per ecosystem were sampled along a transect, in total 93 cores were analysed. Carbohydrates were sequentially extracted using MilliQ-water and 0.3 M EDTA for later analyses. The total carbohydrate content was assessed using the phenol-sulfuric acid assay (Dubois et al., 1956). Briefly, 100 µL of resuspended samples or extracts were mixed with 100 µL of 5% phenol solution, followed by the addition of 500 µL of concentrated sulfuric acid. The reaction mixture was incubated at room temperature for 10 minutes, then further incubated at 30°C for 20 minutes. Absorbance at 490 nm was measured using a Spectramax Id3 plate reader (Molecular Devices) and quantified against a glucose standard curve.

Polysaccharides quantified in sediment cores from coastal vegetated ecosystems

50-cm deep sediment cores were taken in saltmarsh, seagrass, mangroves and unvegetated areas around the German Bight, Malaysia and Columbia in 2022 and 2023. Up to 3 points per ecosystem were sampled along a transect, in total 93 cores were analysed. Carbohydrates were sequentially extracted using MilliQ-water and 0.3 M EDTA for later analyses. Polysaccharides were screened using microarray analysis following the method described by Vidal-Melgosa et al. (2022). Briefly, sediment extracts from MilliQ-water and EDTA were combined in equal volumes, and 30 µL of the mixture was transferred into wells of 384-microwell plates. Two consecutive two-fold dilutions were performed using a printing buffer (55.2% glycerol, 44% water, 0.8% Triton X-100). The plates were then centrifuged at 3,500 × g for 10 minutes at 15 °C. Each microarray was individually probed with a monoclonal antibody (mAb), and binding was detected using a secondary antibody conjugated to alkaline phosphatase. In the presence of its substrate, this reaction produced a colorimetric signal. Developed arrays were scanned at 2400 dots per inch, and binding signal intensity was quantified using Array-Pro Analyzer 6.3 software (Media Cybernetics).

Monosaccharides quantified in sediment cores from coastal vegetated ecosystems

50-cm deep sediment cores were taken in saltmarsh, seagrass, mangroves and unvegetated areas around the German Bight, Malaysia and Columbia in 2022 and 2023. Up to 3 points per ecosystem were sampled along a transect, in total 93 cores were analysed. Carbohydrates were sequentially extracted using MilliQ-water and 0.3 M EDTA for later analyses. Extracts were acid hydrolysed (1 M HCl, 24 h, 100°C) and monosaccharides were analysed using anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), according to Engel et al., 2011. Briefly, sample analysis was performed using a Dionex ICS-5000+ system with a CarboPac PA10 analytical column (2 × 250 mm) and a CarboPac PA10 guard column (2 × 50 mm). Neutral and amino sugars were separated under isocratic conditions with 18 mM NaOH, while acidic monosaccharides were separated using a gradient up to 200 mM NaCH₃COO.

Monosaccharides quantified in porewater samples from coastal vegetated ecosystems

Porewater was taken from 30 to 50 cm depths in saltmarsh, seagrass and unvegetated areas around the German Bight in 2022 and 2023. Up to 9 points per ecosystem were sampled along a transect. Polysaccharides >5kDa were upconcentrated using AMICON-filtration devide and afterwards freeze dried. Dired samples were resuspended in MilliQ-water and acid hydrolysed (1 M HCl, 24 h, 100°C). Monosaccharides were analysed using anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD, ThermoFisher Dionex ICS-5000+ system equipped with a CarboPac PA10 analytical column (2 x 250 mm) and a CarboPac PA10 guard column (2 x 50 mm)), according to Engel et al. (2011).

Polysaccharide fucoidan BAM1 and arabinogalactan-protein glycan JIM13 antibody binding in sediment cores from coastal vegetated ecosystems

50-cm deep sediment cores were taken in saltmarsh, seagrass, mangroves and unvegetated areas around the German Bight, Malaysia and Columbia in 2022 and 2023. Up to 3 points per ecosystem were sampled along a transect, in total 93 cores were analysed. Carbohydrates were sequentially extracted using MilliQ-water and 0.3 M EDTA for later analyses. For more specific analysis enzyme-linked immunosorbent assay (ELISA) was used for detection of fucoidan and arabinogalactan-protein glycan as described in the following studies (Vidal-Melgosa et al., 2021 and Cornuault et al., 2014). In short, 100 µL of sediment extracts were added to a pre-coated 96-well plate and incubated overnight at 4°C. The signal was developed using primary antibodies, BAM1 (fucoidan) and JIM13 (arabinogalactan-protein glycan), diluted 1:10 in skim milk PBS solution, followed by anti-rat antibody at a 1:1000 dilution in the same solution. Absorbance was measured at 450 nm using a Spectramax Id3 plate reader (Molecular Devices).

Polysaccharide fucoidan BAM1 antibody binding in porewater samples from coastal vegetated ecosystems

Porewater of saltmarsh, seagrass and unvegetated areas around the German Bight in 2022 and 2023 was sampled at 50cm depth. A transect of up to 9 points per ecosystem were sampled. Polysaccharides >5kDa were upconcentrated using AMICON-filtration device, freeze dried and dried samples were resuspended in MilliQ-water. Polysaccharides were screened for fucoidan BAM1 antibody binding using ELISA method, according to Cornuault et al. (2014).

TrilaWatt: Hydrodynamik (2015-2022)

<strong>Definitionen:</strong> Hydrodynamik beschreibt die Bewegung von Fluiden und die dabei wirkenden Kräfte. <strong>Datenerzeugung:</strong> Die veröffentlichten Daten basieren auf validierten, hydronumerischen Modellsimulationen und werden von unstrukturierten Modellergebnissen auf regelmäßige Raster inter- und extrapoliert. <strong>Produkte:</strong> Für jedes Jahr stehen für die Deutsche Bucht, das niederländische und dänische Wattenmeer 4 netCDF-Dateien im Download-Bereich zur Verfügung. Die Dateien haben folgenden Inhalt: - Tidedynamik (tides): Wasserstand und tiefengemittelte Strömungsgeschwindigkeit - Transport (transport): Salzgehalt, Temperatur und Schwebstoffgehalt (alle tiefengemittelt, an der Gewässersohle und an der Wasseroberfläche) - Seegang (waves): signifikante Wellenhöhe, mittlere Wellenperiode, Peak Wellenperiode, Wellenperiode 1. und 2. Moment, mittlere Wellenrichtung und die Richtungsaufweitung - Bodenschubspannung (shear_stress) <strong>Literatur:</strong> Lepper, R., Reinert, M., Gundlach, J., Weber, J., Kösters, F. (2025): A hydrographic dataset of the Wadden Sea as a foundation for a digital twin of the coastal ocean. https://doi.org/10.1038/s41597-025-06211-1 <strong>English:</strong> The map service TrilaWatt: Hydrodynamic (WMS) contains tidal dynamics, sea water salinity, sea water temperature, suspended sediment concentration, bed shear stress and waves on a 500m regular grid in 20-minute intervals. <strong>Download:</strong> A download is located under references (in German: "Verweise und Downloads").

TrilaWatt: Hydrodynamische Kennwerte 2019 (WMS)

<span><strong>Definitionen:</strong> Hydrodynamik beschreibt die Bewegung von Fluiden und die dabei wirkenden Kräfte. Hydrodynamische Kennwerte sind zeitintegrierte, beschreibende Parameter dieser Prozesse. So tragen bspw. die grundlegenden Tidekenngrößen des Tidehochwassers, des Tideniedrigwassers sowie der damit eng verbundenen Werte für Tidestieg, Tidefall und Tidehub dazu bei, die Dynamik der Tide herauszuarbeiten.</span> <span><strong>Datenerzeugung:</strong> Aus numerischen Simulationsdaten wurden physikalische Größen wie beispielsweise Wasserstand oder Strömungsgeschwindigkeit in festen zeitlichen Intervallen unter Berücksichtigung erreichbarer Genauigkeiten berechnet. Diese Simulationsdaten wurden mit Datenanalysemethoden zu hydrodynamischen Kennwerten wie beispielsweise dem Tidehub zusammengefasst. Es wurden harmonische Analysen des Wasserstandes durchgeführt und Tidekennwerte des Wasserstands bzw. statistische Langzeitkennwerte von Wasserstand, Strömungsgeschwindigkeit, Salzgehalt, Wassertemperatur und Schwebstoffgehalt berechnet. </span> <span><strong>Produkte:</strong> Hydrodynamische Kennwerte aus dem Projekt TrilaWatt basieren auf der Analyse der numerischen Simulation von Tide, Seegang, Salzgehalt, Temperatur und Schwebstoffkonzentration im Bereich des trilateralen Wattenmeers (Niederlande -nl, Deutschland -de, Dänemark -dk) und der Deutschen Bucht als Jahresmittel für das Jahr 2019. Die Daten werden als regelmäßiges 20 m Raster im GeoTIFF-Format bereitgestellt. Kennwerte werden nur für Berechnungszellen bereitgestellt, die im Analysezeitraum immer überflutet waren. In den Datenäquivalenten (*_no_filter) wurde diese Maskierung nicht angewendet. Nicht-gefilterte Datenäquivalente (no_filter) sind, falls physikalisch sinnvoll, ebenfalls erstellt worden. Bei nicht-gefilterten Datenprodukten ist zu beachten, dass die Anzahl der den Mittelwerten zugrundeliegenden Werte vor allem im Flachwasserbereich durch intertidales Trockenfallen geringer ist und damit die Mittelwertbildung beeinträchtigt ist. Die Anzahl an validen Datenpunkten bzw. Tiden pro Jahr (Anzahl gültiger Datenpunkte bzw. Anzahl Tidehochwasser) wird als Rasterdatei zur Einordnung nicht-gefilterter Produkte mitgeliefert.</span> <span><strong>Produktliste:</strong> - Tidehub und Tidehoch- und Tideniedrigwasser: 5-, 50- und 95% Quantil <br> - Laufzeitverschiebung zur Referenzposition „Leuchtturm Alte Weser“ von Tidehoch- und Tideniedrigwasser: Jahresmittelwerte <br> - Tidemittelwasser: 50% Quantil <br> - M2-Partialtide: Amplitude und Phase <br> - Tidehochwasser und validen Datenpunkte: Anzahl pro Jahr<br> - Wasserstand: 1-, 50- und 99% Quantil, Mittelwert, Minimum, Maximum <br> - Strömungsgeschwindigkeit: tiefengemittelter Mittelwert, 99- und 99,9% Quantil des Betrags <br> - Strömungsgeschwindigkeit: tiefengemittelter Betrag und x- und y-Komponente des Residuums <br> - Strömungsgeschwindigkeit: tiefengemittelter mittlerer, kubierter Betrag <br> - Bodenschubspannung: 99% Quantil, Mittelwert<br> - Salzgehalt, Temperatur und Schwebstoffkonzentration: tiefengemitteltes 1- und 99% Quantil und Mittelwert (Schwebstoffkonzentration als Summe aus drei Fraktionen mit einer Sinkgeschwindigkeit ws = 0,25, 1,5 und 7 mm/s) <br> - Signifikante Wellenhöhe des Seegangs: 50-, 95- und 99% Quantil, (Jahres-) Mittelwert und Maximalwert <br> - Mittlere Wellenperiode: Jahresmittelwert bei maximaler signifikanter Wellenhöhe<br> - Seegangsrichtung: x- und y-Komponenten des Residuums </span> <span><strong>English:</strong> This web service contains annual averages and quantiles of tidal characteristics, annual averages and quantiles of hydrographic parameters (e.g., depth-averaged salinity, suspended sediments, or sea water temperature), and tidal constituents from harmonic analyses that were estimated from numerical simulations of the year 2019. Data are distributed on regular 20 m grids as GeoTIFFs. </span> <span><strong>Download:</strong> A download is located under references (in German: "Verweise und Downloads"). </span>

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