We consider clay minerals, iron oxides and charcoal as major components controlling the formation of interfaces relevant for sorption of organic chemicals, as they control the assemblage of organic matter and mineral particles. We studied the formation of interfaces in batch incubation experiments with inoculated artificial soils consisting of model compounds (clay minerals, iron oxide, char) and natural soil samples. Results show a relevant contribution of both iron oxides and clay minerals to the formation of organic matter as sorptive interfaces for hydrophobic compounds. Thus, we intend to focus our work in the second phase on the characterization of the interface as formed by organic matter associated with clay minerals and iron oxides. The interfaces will be characterized by the BET-N2 and ethylene glycol monoethyl ether (EGME) methods and 129Xe and 13C NMR spectroscopy for determination of specific surface area, sorptive domains in the organic matter and microporosity. A major step forward is expected by the analysis of the composition of the interface at different resolution by reflected-light microscopy (mm scale), SEM (scanning electron microscopy, micrometer scale) and secondary ion mass spectrometry at the nanometer scale (nanoSIMS). The outcomes obtained in combination with findings from cooperation partners will help to unravel the contribution of different types of soil components on the formation and characteristics of the biogeochemical interfaces and their effect on organic chemical sorption.
In bog ecosystems, vegetation controls key processes such as the retention of carbon, water and nutrients. In northern hemispherical bogs, a shift from Sphagnum- to vascular plant-dominated vegetation is often traced back to Climate Change and increased anthropogenic nitrogen deposition and coincides with substantially reduced capacities in carbon, water and nutrient retention. In southern Patagonia, bogs dominated by Sphagnum and vascular plants coexist since millennia under similar environmental settings. Thus, South Patagonian bogs may serve as ideal examples for the long-term effect of vascular plant invasion on carbon, water and nutrient balances of bog ecosystems. The contemporary balances of carbon and water of both a bog dominated by Sphagnum and vascular plants are determined by CO2- H2O and CH4 flux measurements and an estimation of lateral water losses as well as losses via dissolved organic and inorganic carbon compounds. The high time resolution of simultaneous eddy covariance measurements of CO2 and H2O in both bog types and the strong interaction between climatic variables and the physiology of bog plants allow for direct comparisons of carbon and water fluxes during cold, warm, dry, wet, cloudy or sunny periods. By the combination with leaf-scale measurements of gas exchange and fluorescence, plant-physiological controls of photosynthesis and transpiration can be identified. Long-term peat accumulation rates will be determined by carbon density and age-depth profiles including a characterization of peat humification characteristics. A reciprocal transplantation experiment with incorporated shading, liming and labeled N addition treatments is conducted to explore driving factors affecting competition between Sphagnum and vascular plants as well as the interactions between CO2-, CH4-, and water fluxes and decisive plant functional traits affecting key processes for carbon sequestration and nutrient cycling. Decomposition rates and driving below ground processes are analyzed with a litter bag field experiment and an incubation experiment in the laboratory.
Cherry leaf roll virus (CLRV) is a plant pathogen of economic and ecologic importance. It is globally distributed in a wide range of forest, fruit, and ornamental trees and shrubs. In several areas of cherry and walnut production CLRV causes severe losses in yield and quality. With current reference to the rapid dissemination and strong symptom expression in Finnish birches and the Germany-wide distribution of CLRV in birches and elderberry, we continuously investigate and gradually reveal CLRV transmission pathways as by pollen, seeds or water. However, modes and interactions responsible for the wide intergeneric host transmission as well as for the exceptional CLRV epidemic in Fennoscandia still remain unknown. In this project systematic studies shall investigate biological vectors as a causal agent to finally derive control mechanisms and strategies to avoid new epidemics in different hosts and geographic regions. Detailed monitoring of the invertebrate fauna of birch stands/forests and elderberry plantations in Germany and Finland shall reveal potential vectors to subsequently study them in detail by approved virus detection methods and transmission experiments. Molecular analyses of the CLRV coat protein shall prove its role as a viral determinant for a virus/vector interaction. Consequently, this project essentially will contribute important answers on the CLRV epidemiology, and this will be a key element within the first network of research on plant viral pathogens in forest trees.
It has been suggested that dying and decaying fine roots and root exudation represent important, if not the most important, sources of soil organic carbon (SOC) in forest soils. This may be especially true for deep-reaching roots in the subsoil, but precise data to prove this assumption are lacking. This subproject (1) examines the distribution and abundance of fine roots (greater than 2 mm diameter) and coarse roots (greater than 2 mm) in the subsoil to 240 cm depth of the three subsoil observatories in a mature European beech (Fagus sylvatica) stand, (2) quantifies the turnover of beech fine roots by direct observation (mini-rhizotron approach), (3) measures the decomposition of dead fine root mass in different soil depths, and (4) quantifies root exudation and the N-uptake potential with novel techniques under in situ conditions with the aim (i) to quantify the C flux to the SOC pool upon root death in the subsoil, (ii) to obtain a quantitative estimate of root exudation in the subsoil, and (iii) to assess the uptake activity of fine roots in the subsoil as compared to roots in the topsoil. Key methods applied are (a) the microscopic distinction between live and dead fine root mass, (b) the estimation of fine and coarse root age by the 14C bomb approach and annual ring counting in roots, (c) the direct observation of the formation and disappearance of fine roots in rhizotron tubes by sequential root imaging (CI-600 system, CID) and the calculation of root turnover, (d) the measurement of root litter decomposition using litter bags under field and controlled laboratory conditions, (e) the estimation of root N-uptake capacity by exposing intact fine roots to 15NH4+ and 15NO3- solutions, and (f) the measurement of root exudation by exposing intact fine root branches to trap solutions in cuvettes in the field and analysing for carbohydrates and amino acids by HPLC and Py-FIMS (cooperation with Prof. A. Fischer, University of Trier). The obtained data will be analysed for differences in root abundance and activity between subsoil (100-200 cm) and topsoil (0-20 cm) and will be related to soil chemical and soil biological data collected by the partner projects that may control root turnover and exudation in the subsoil. In a supplementary study, fine root biomass distribution and root turnover will also be studied at the four additional beech sites for examining root-borne C fluxes in the subsoil of beech forests under contrasting soil conditions of different geological substrates (Triassic limestone and sandstone, Quaternary sand and loess deposits).
In hydrology, the relationship between water storage and flow is still fundamental in characterizing and modeling hydrological systems. However, this simplification neglects important aspects of the variability of the hydrological system, such as stable or instable states, tipping points, connectivity, etc. and influences the predictability of hydrological systems, both for extreme events as well as long-term changes. We still lack appropriate data to develop theory linking internal pattern dynamics and integral responses and therefore to identify functionally similar hydrological areas and link this to structural features. We plan to investigate the similarities and differences of the dynamic patterns of state variables and the integral response in replicas of distinct landscape units. A strategic and systematic monitoring network is planned in this project, which contributes the essential dynamic datasets to the research group to characterize EFUs and DFUs and thus significantly improving the usual approach of subdividing the landscape into static entities such as the traditional HRUs. The planned monitoring network is unique and highly innovative in its linkage of surface and subsurface observations and its spatial and temporal resolution and the centerpiece of CAOS.
Cydia pomonella granulovirus (CpGV, Baculoviridae) is one of the most important agents for the control of codling moth (CM, Cydia pomonella, L.) in both biological and integrated pest management. The rapid emergence of resistance against CpGV-M, which was observed in about 40 European CM field populations from 2003 on, could be traced back to a single, dominant, sex-linked gene. Since then, resistance management has been based on mixtures of new CpGV isolates (CpGV-I12, -S), which are able to overcome this resistance. Recently, resistance even to these novel isolates was observed in CM field populations. This resistance does not follow the described dominant, sex-linked inheritance trait. At the same time, another isolate CpGV-V15 was identified showing high virulence against these resistant populations. To elucidate this novel resistance mechanism and to identify the resistance gene(s) involved, we propose a comprehensive analysis of this resistance on the cellular and genomic level of codling moth. Because of the lack of previous knowledge of the molecular mechanisms of virus resistance in insects, several different and complementary approaches will be pursued. This study will not only give an in-depth insight into the genetic possibilities for development of baculovirus resistance in CM field populations and how the virus overcomes it, but can also serve as an important model for other baculovirus-host interaction systems.
Especially during the last decades, the natural forests of Ethiopia have been heavily disturbed by human activities. Some forests have been totally cleared and converted into fields for agricultural use, other suffered from different influences, such as heavy grazing and selective logging. The ongoing research in the Shashemane-Munessa-study area (Gu 406/8-1,2) showed clearly that, in spite of interdiction and control, forests continue to be cleared and degraded. However, it is not yet sufficiently known, how and why these processes are still going on. Growing population pressure and economic constraints for the people living in and around the forests contribute to the actual situation but allow no final answers to the complex situation. Concerning a sustainable management of the forests there is to no solid basis for recommendations from the socioeconomic and socio-cultural view. Therefore, a comprehensive analysis of the traditional needs and forms of forest use, including all forest products, is necessary. The objective of this project is, to achieve this basis by carrying out intensive field observations, the consultation of aerial photographs, satellite imagery and above all semi-structured interviews with the population in the study area in order to contribute to the recommendations for a sustainable use of the Munessa Shasemane forests.
In structured soils, the interaction of percolating water and reactive solutes with the soil matrix is mostly restricted to the surfaces of preferential flow paths. Flow paths, i.e., macropores, are formed by worm burrows, decayed root channels, cracks, and inter-aggregate spaces. While biopores are covered by earthworm casts and mucilage or by root residues, aggregates and cracks are often coated by soil organic matter (SOM), oxides, and clay minerals especially in the clay illuviation horizons of Luvisols. The SOM as well as the clay mineral composition and concentration strongly determine the wettability and sorption capacity of the coatings and thus control water and solute movement as well as the mass exchange between the preferential flow paths and the soil matrix. The objective of this proposal is the quantitative description of the small-scale distribution of physicochemical properties of intact structural surfaces and flow path surfaces and of their distribution in the soil volume. Samples of Bt horizons of Luvisols from Loess will be compared with those from glacial till. At intact structural surfaces prepared from soil clods, the spatial distribution (mm-scale) of SOM and clay mineral composition will be characterized with DRIFT (Diffuse reflectance infrared Fourier transform) spectroscopy using a self-developed mapping technique. For samples manually separated from coated surfaces and biopore walls, the contents of organic carbon (Corg) and the cation exchange capacity (CEC) will be analyzed and related to the intensities of specific signals in DRIFT spectra using Partial Least Square Regression (PLSR) analysis. The signal intensities of the DRIFT mapping spectra will be used to quantify the spatial distribution of Corg and CEC at these structural surfaces. The DRIFT mapping data will also be used for qualitatively characterizing the small scale distribution of the recalcitrance, humification, and microbial activity of the SOM from structural surfaces. The clay mineral composition of defined surface regions will be characterized by combining DRIFT spectroscopic with X-ray diffractometric analysis of manually separated samples. Subsequently, the spatial distribution of the clay mineral composition at structural surfaces will be determined from the intensities of clay mineral-specific signals in the DRIFT mapping spectra and exemplarily compared to scanning electron microscopic and infrared microscopic analysis of thin sections and thin polished micro-sections. The three-dimensional spatial distribution of the total structural surfaces in the volume of the Bt horizons will be quantified using X-ray computed tomography (CT) analysis of soil cores. The active preferential flow paths will be visualized and quantified by field tracer experiments. These CT and tracer data will be used to transfer the properties of the structural surfaces characterized by DRIFT mapping onto the active preferential flow paths in the Bt horizons.
Unsere Motivation liegt in der Tatsache, dass die dynamische Verbindung zwischen dem marinen Oberflächenfilm (engl. sea-surface microlayer, SML) und der darunterliegenden oberflächennahen Wasserschicht über Konvektion zu heterogenen Eigenschaften der SML führt. Dies wiederum steuert das Ausmaß der bio-photochemischen Reaktionen und des Gasaustausches zwischen dem Ozean und der Atmosphäre. Die Konvektion wird durch Verdunstung angetrieben, die die SML abkühlt und es salzhaltiger macht. Infolgedessen wird die SML dichter, sinkt ab und wird durch das darunterliegende Wasser ersetzt. Die auftriebsgetriebene Konvektion wurde jedoch bei der Erforschung der SML und des Gasaustausches als dynamisches Bindeglied zwischen der Atmosphäre und dem Ozean vernachlässigt. Unser Hauptziel ist es, ein mechanistisches Verständnis der Dynamik zwischen der SML und der oberflächennahen Wasserschicht zu beschreiben. Ein mechanistisches Verständnis der Konvektion ist wichtig, da das Ausmaß der bio-photochemischen Reaktionen und Austauschprozessen von Spurengasen, Energie und Impuls letztlich durch Austauschprozesse zwischen der SML und der oberflächennahen Wasserschicht und schließlich mit tieferen Schichten bestimmt wird. Wir werden einen experimentellen Aufbau mit mehreren profilierenden Mikroelektroden und einem optischen Schlierensystem entwickeln, um die Konvektion unter verschiedenen externen Antrieben zu untersuchen. Wir werden den Effekt der horizontalen Strömung aufgrund von Gradienten der Oberflächenspannung (d.h. Marangoni-Effekt) untersuchen. Wir werden auch an dem gemeinsamen Mesokosmen-Experiment BASS teilnehmen, um den Einfluss biogener Tenside auf den konvektiven Transportmechanismus zwischen der SML und der oberflächennahen Wasserschicht zu untersuchen. Im gemeinsamen Feldexperiment BASS werden wir der Frage nachgehen, inwieweit Variationen der klein-skaligen Konvektion durch die Variabilität sub-mesoskaligen (1 km-10 km) und hydrodynamischen Prozessen nahe der Meeresoberfläche beeinflusst werden. Wir werden zwei Forschungskatamarane und eine Flotte von Treibbojen einsetzen, die mit Leitfähigkeits- und Temperatursensoren ausgestattet sind, um Dichteanomalien zwischen der SML und oberflächennahen Wasserschicht zu untersuchen. Wir werden externe ozeanische und atmosphärische Einflüsse beobachten, um die Dichteanomalien zu beschreiben. Schließlich werden wir die gewonnenen Erkenntnisse aus den Laborexperimenten, der Mesokosmos-Studie und der Feldstudie nutzen, um einen mathematischen Rahmen zur Beschreibung von Temperatur- und Salzgehaltsprofilen und deren Schwankungen unter dem Einfluss definierter ozeanischer und atmosphärischer Einflüsse zu entwickeln.
The hypothesis that the dark septate root endophyte Phialocephala fortinii provides biological control of Phytophthora diseases to trees is tested under two temperature regimes. Phytophthora spp. are very aggressive plant pathogens affecting also woody plants in the natural environment. Studies on biological control of Phytophthora diseases are scarce, but antagonistic effects of endophytic fungi have been demonstrated in a few cases. In temperate and boreal forests, conifers are much less susceptible to Phytophthora diseases than deciduous broadleaf trees, whereas their roots are very frequently colonized by dark septate endophytes (DSE) with Phialocephala fortinii s.l., a supposedly asexual ascomycete, being the dominant component. This fungus also colonizes hardwoods, but to a lesser degree. Recent population genetic studies showed that P. fortinii s.l. consists of at least 15 reproductively isolated cryptic species (CSP). We hypothesise that P. fortinii s.l. may protect its host from Phytophthora disease depending on CSP and environmental conditions. A model system will be developed and applied to test this hypothesis using Norway spruce (Picea abies), beech (Fagus sylvatica) and Phytophthora citricola. The experimental program will be multidisciplinary in approach and include molecular, mycological, phytopathological (infection experiments, epifluorescence microscopy) and statistical methods.
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