In this experiment Syngnathus typhle individuals were collected at six different locations along the German Baltic Sea coastline. To analyse the populations structure of the pipefish we isolated genomic DNA from fin clips of collected pipefish female. All 144 female S. typhle samples were genotyped for 11 microsatellite loci, with a minimum of 20 individuals per sampling site. The allele report contains the microsatelite length of the three primer pools used for multiplex PCR.
The report gives information, if indivuduals are homozygous (same allele length) or heterozygous (different allele length) for targeted alleles. Some samples were negelected due to too many missing alleles.
Allele reports were uploaded in Microchecker and Genetix for analyses of the population structure.
The aim of the study was to investigate the mechanisms allowing pipefish to inhabit low salinity areas of the Baltic Sea.
The parental Syngnathus typhle generation for this experiment was caught in seagrass meadows of six sampling sites along the German coastline of the Baltic Sea in spring 2017, i.e. in the Flensburg Fjörd, Falckensteiner Strand, Orth Bay next to Fehmarn, Salzhaff and 2 sample sites around Rügen. Three sampling sites are characterized by relatively high salinity conditions (14 - 17 PSU; high salinity origin; H) and three sampling sites by relatively low salinity conditions (7 - 11 PSU; low salinity origin, L; Table 1). Salzhaff was assigned the category low because salinity drops are common after rainfall accompanied with freshwater discharge due to enclosed morphology of the inlet. Therefore, pipefish in Salzhaff are likely to be exposed to salinity levels below 10 PSU. A minimum of 30 non-pregnant males and 30 females were caught snorkelling with hand nets at each sampling site at depths ranging between 0.5 and 2.5 m. At each sampling site, water temperature and salinity were measured from water collected about 1 m below the surface using a salinometer (WTW Cond 330i).
The common garden experiment was conducted at the facilities of the GEOMAR (west shore) in spring 2020.
We investigated effects of temperature during ontogeny of the kelp Laminaria digitata across haploid gametophyte and diploid sporophyte life cycle stages in 5 distinct genetic lines. We sampled meristematic discs from wild sporophytes on the island of Helgoland (North Sea; 54.1779 N, 7.8926 E) in May 2017, July 2017 and February 2018 and conducted a seasonal growth experiment at 5°C and 15°C over 14 days (experiment 1). Based on meiospores from five individuals (genetic lines) sampled in July 2017, we applied a full-factorial experimental design to generate different temperature histories by applying 5°C and 15°C during meiospore germination, gametogenesis of parental gametophytes and recruitment of offspring sporophytes (19-26 days; recruitment), and juvenile sporophyte rearing (91-122 days). We then tested for thermal plasticity among temperature history treatments at 5°C and 15°C in a final 12-day experiment assessing growth, the storage compound mannitol, carbon and nitrogen contents, and fluorometric responses in 3-4 month old sporophytes (experiment 2).
Die Auswirkungen unterschiedlicher ackerbaulicher Nutzungsgeschichte auf die genetische Struktur von Populationen ausgewählter annueller Ackerwildkräuter (Arabidopsis thaliana, Gnaphalium uliginosum, Viola arvensis) werden untersucht. Parallel dazu werden ihre phänotypische Plastizität und ökologische Fitness ermittelt. Im Vordergrund steht dabei die Hypothese, dass sich die genetische Architektur der untersuchten Populationen und - damit einhergehend - ihre phänotypische Plastizität und Fitness aufgrund moderner Produktionsmethoden im Laufe der letzten Jahrzehnte verändert haben. Dabei soll der Einfluß der Herbizidanwendung besonders berücksichtigt werden. Mit Hilfe von Stichproben aus der Diasporenbank unterschiedlich alter, zuvor als Acker genutzter Wiesen sowie noch heute bewirtschafteter Flächen einer Extensiv- und einer Intensivagrarlandschaft werden die Auswirkungen unterschiedlicher Nutzungsgeschichte auf die Populationsstruktur erfaßt. Beziehungen zwischen genetischer Diversität, phänotypischer Plastizität und Fitness werden durch die gekoppelte Erhebung der genetischen und phänotypischen Daten hergestellt. Die Untersuchungen zur genetischen Diversität erfolgen mit AFLP-Markern, die Analyse der intraspezifischen Populationsstruktur an Arabidopsis mit Hilfe von Mikrosatelliten. Phänotypische Plastizität und Fitness der Populationen werden aus populationsbiologischen Erhebungen abgeleitet.