Fusarium species of the Gibberella fujikuroi species complex cause serious diseases on different crops such as rice, wheat and maize. An important group of plant pathogens is the Gibberella fujikuroi species complex (GFC) of closely related Fusarium species which are associated with specific hosts; F. verticillioides and F. proliferatum are particularly associated with maize where they can cause serious ear-, root-, and stalk rot diseases. Two other closely related species of the GFC, F. mangiferae and F. fujikuroi, which share about 90Prozent sequence identity with F. verticillioides, are pathogens on mango and rice, respectively. All of these species produce a broad spectrum of secondary metabolites such as phytohormones (gibberellins, auxins, and cytokinins), and harmful mycotoxins, such as fumonisin, fusarin C, or fusaric acid in large quantities. However, the spectrum of those mycotoxins might differ between closely related species suggesting that secondary metabolites might be determinants for host specificity. In this project, we will study the potential impact of secondary metabolites (i.e. phytohormones and certain mycotoxins) and some other species-specific factors (e.g. species-specific transcription factors) on host specificity. The recently sequenced genomes of F. mangiferae and F. fujikuroi by our groups and the planned sequencing of F. proliferatum will help to identify such determinants by genetic manipulation of the appropriate metabolic pathway(s).
The present-day configuration of Indonesia and SE Asia is the results of a long history of tectonic movements, volcanisms and global eustatic sea-level changes. Not indifferent to these dynamics, fauna and flora have been evolving and dispersing following a complicate pattern of continent-sea changes to form what are today defined as Sundaland and Wallacea biogeographical regions. The modern intraannual climate of Indonesia is generally described as tropical, seasonally wet with seasonal reversals of prevailing low-level winds (Asian-Australian monsoon). However at the interannual scale a range of influences operating over varying time scales affect the local climate in respect of temporal and spatial distribution of rainfall. Vegetation generally reflects climate and to simplify it is possible to distinguish three main ecological elements in the flora of Malaysia: everwet tropical, seasonally dry tropical (monsoon) and montane. Within those major ecological groups, a wide range of specific local conditions caused a complex biogeography which has and still attract the attention of botanists and biogeographers worldwide. Being one of the richest regions in the Worlds in terms of species endemism and biodiversity, Indonesia has recently gone through intensive transformation of previously rural/natural lands for intensive agriculture (oil palm, rubber, cocoa plantations and rice fields). Climate change represents an additional stress. Projected climate changes in the region include strengthening of monsoon circulation and increase in the frequency and magnitude of extreme rainfall and drought events. The ecological consequences of these scenarios are hard to predict. Within the context of sustainable management of conservation areas and agro-landscapes, Holocene palaeoecological and palynological studies provide a valuable contribution by showing how the natural vegetation present at the location has changed as a consequence of climate variability in the long-term (e.g. the Mid-Holocene moisture maximum, the modern ENSO onset, Little Ice Age etc.). The final aim of my PhD research is to compare the Holocene history of Jambi province and Central Sulawesi. In particular: - Reconstructing past vegetation, plant diversity and climate dynamics in the two study areas Jambi (Sumatra) and Lore Lindu National Park (Sulawesi) - Comparing the ecological responses of lowland monsoon swampy rainforest (Sumatra) and everwet montane rainforests (Sulawesi) to environmental variability (vulnerability/resilience) - Investigating the history of human impact on the landscape (shifting cultivation, slash and burn, crop cultivation, rubber and palm oil plantation) - Assessing the impact and role of droughts (El Niño) and fires - Adding a historical perspective to the evaluation of current and future changes.
The nature of the microbial communities inhabiting the deeper soil horizons is largely unknown. It is also not clear why subsurface microorganisms do not make faster use of organic compounds under field conditions. The answer could be provided by a reciprocal soil transfer experiment studying the response of transferred soils to fluctuations in microclimate, organic inputs, and soil biota. The subproject P9 will be responsible for the establishment of reciprocal transfer experiments offering a strong link between subgroups interested in organic matter quality, transport of organic substances, as well as functions of the soil microbial community. A single, high molecular weight substrate (13C labelled cellulose) will be applied at two different levels in the pre-experiment to understand the dose-dependent reaction of soil microorganisms in transferred surface and sub-soils. Uniformly 13C labelled beech roots - representing complex substrates - will be used for the main reciprocal soil transfer experiment. We hypothesize that transferring soil cores between subsoil and surface soil as well as addition of labelled cellulose or roots will allow us to evaluate the relative impact of surface/subsurface habitat conditions and resource availability on abundance, function, and diversity of the soil microbial community. The second objective of the subproject is to understand whether minerals buried within different soil compartments (topsoil vs. subsoil) in the field contribute to creation of hot spots of microbial abundance and activity within a period of two to five years. We hypothesize that soil microorganisms colonize organo-mineral complexes depending on their nutritional composition and substrate availability. The existence of micro-habitat specific microbial communities could be important for short term carbon storage (1 to 6 years). The third objective is to understand the biogeography and function of soil microorganisms in different subsoils. Parent material as well as mineral composition might control niche differentiation during soil development. Depending on size and interconnectedness of niches, colonization and survival of soil microbial communities might be different in soils derived from loess, sand, terra fusca, or sandstone. From the methodological point of view, our specific interest is to place community composition into context with soil microbial functions in subsoils. Our subgroup will be responsible for determining the abundance, diversity, und function of soil microorganisms (13C microbial biomass, 13C PLFA, enzyme activities, DNA extraction followed by quantitative PCR). Quantitative PCR will be used to estimate total abundances of bacteria, archaea and fungi as well as abundances of specific groups of bacteria at high taxonomic levels. We will apply taxa specific bacterial primers because classes or phyla might be differentiated into ecological categories on the basis of their life strategies.
Der schnelle Fortschritt der elektronischen Geräte erhöht die Nachfrage nach verbesserten Li-Ionen Batterien. Kommerziell erhältliche Li-Zellen nutzen meist Lithiumkobaltoxid für die positive Elektrode. Doch gerade dieses Material ist ein Hindernis für eine weitere Optimierung, insbesondere für eine Kostensenkung. Vor allem für größere Anwendungen wie Hybrid- oder Elektrofahrzeuge müssen alternative Materialen erforscht werden, die billiger, sicherer und umweltverträglicher sind. Daher wird im ISEA derzeit ein neues Forschungsprojekt ins Leben gerufen und die dafür benötigte Infrastruktur geschaffen. Die Forschung wird sich auf die Untersuchung geeigneter Übergangsmetalloxide und Polyanionen konzentrieren, die besonders gut zur Einlagerung von Li-Ionen geeignet sind. Es werden neue Herstellungsverfahren unter Verwendung wässriger Precurser-Substanzen untersucht, die Verbindungen mit überlegenen Eigenschaften erzeugen und außerdem leicht an eine Massenproduktion angepasst werden können. Ziel der Arbeiten ist, preisgünstiges Elektrodenmaterial zu entwickeln, das eine spezifische Energie von über 200 Wh/kg und eine Leistungsdichte von 400 W/kg aufweist. Außerdem werden Arbeiten im Bereich der physikalisch-chemischen Charakterisierung der neuen Materialien stattfinden sowie elektrochemische Analysen der gesamten Zellen- und Batteriesysteme durchgeführt. Das elektrodynamische Verhalten der neuen Zellen wird u. a. mit Hilfe der elektrochemischen Impedanzspektroskopie analysiert, um präzise und zuverlässige Algorithmen für ein späteres Batteriemonitoring im realen Betrieb zu finden.
The magnetosphere of a planet is controlled by a number of factors such as the intrinsic magnetic field, the atmosphere and ionosphere, and the solar wind. Different combinations of these control factors are at work at the terrestrial planets Mercury, Venus, Earth, and Mars, hence they form a very suitable set for quantitative comparative studies. A significant intrinsic dipolar magnetic field is present only on Earth and on Mercury. However, the configuration at Mercury differs considerably from that at Earth because Mercury does not support an atmosphere and ionosphere, the dipolar field is much weaker, the solar wind denser, and the interplanetary magnetic field stronger. Both Mars and Venus have atmospheres but lack a global planetary magnetic field, with regional crustal magnetization being present on Mars. This proposal aims at investigating and comparing electrical current systems in the space environments of terrestrial planets using magnetic vector data collected by orbiting spacecraft such as Venus Express, Mars Global Surveyor, CHAMP (Earth), and MESSENGER (Mercury). We propose to construct data-driven and physically meaningful representations that reveal and quantify the influence of various control factors. To achieve this, we will tailor Empirical Orthogonal Function (EOF) analysis and other multivariate methods to the specifics of planetary magnetic field observations. In contrast to representations that build on predefined functions like spherical harmonics, basis functions in the EOF approach are derived directly from the data. EOFs are designed to extract dominant coherent variations for further interpretation in terms of known physical phenomena, and then, in a regression step, for modeling using suitable control variables. The EOF methodology thus allows quantifying the relative importance of control factors for each planet individually, and thus contributes to the solution of topical science questions. The resulting empirical models will facilitate comparative studies of current systems at the terrestrial planets.
Comprehension of belowground competition between plant species is a central part in understanding the complex interactions in intercropped agricultural systems, between crops and weeds as well as in natural ecosystems. So far, no simple and rapid method for species discrimination of roots in the soil exists. We will be developing a method for root discrimination of various species based on Fourier Transform Infrared (FTIR)-Attenuated Total Reflexion (ATR) Spectroscopy and expanding its application to the field. The absorbance patterns of FTIR-ATR spectra represent the chemical sample composition like an individual fingerprint. By means of multivariate methods, spectra will be grouped according to spectral and chemical similarity in order to achieve species discrimination. We will investigate pea and oat roots as well as maize and barnyard grass roots using various cultivars/proveniences grown in the greenhouse. Pea and oat are recommendable species for intercropping to achieve superior grain and protein yields in an environmentally sustainable manner. To evaluate the effects of intercropping on root distribution in the field, root segments will be measured directly at the soil profile wall using a mobile FTIR spectrometer. By extracting the main root compounds (lipids, proteins, carbohydrates) and recording their FTIR-ATR spectra as references, we will elucidate the chemical basis of species-specific differences.
In Fragebogen-Untersuchungen zur Lärmwirkung wurden bisher sehr unterschiedliche Operationalisierungen von Wirkungsvariablen (wie z.B. Belästigung, Störung von Aktivitäten) und außer-akustischen Faktoren (sog. Moderatoren wie z.B. Lärmempfindlichkeit, misfeasance) verwendet. Deshalb hat sich die Arbeitsgruppe community response der International Commission on the Biological Effects of Noise (ICBEN, Team No. 6) als langfristiges Ziel die Entwicklung von Fragebogen-Guidelines und die Formulierung eines Muster-Fragebogens für die Lärmwirkungsforschung gesetzt. D.h. es soll ein Vorschlag erarbeitet werden, in welcher Form globale und spezifische Lärmwirkungen in Befragungen erhoben werden sollten. Um dieses Vorhaben zu unterstützen, hat der Arbeitskreis Ökologische Lärmforschung die Erstellung einer systematischen Übersicht über vorhandene Fragebögen aus Lärmwirkungsstudien auf internationaler Ebene in Angriff genommen. Diese Übersicht soll es u.a. ermöglichen, die Struktur von verschiedenen Fragebögen sowie die in ihnen verwendeten Operationalisierungen für Lärmwirkungs- und Moderatorvariablen (hinsichtlich Art der Frageformulierung sowie der Antwortformate) zu vergleichen. Für den/die einzelne/n Lärmforscher/in bietet diese Übersicht die Möglichkeit, sich auf sehr effiziente Art und Weise darüber zu informieren, wie bestimmte Konstrukte in bisherigen Untersuchungen operationalisiert worden sind bzw. welche Alternativen zu den bereits selbst angewandten Operationalisierungen bestehen. Nach einer systematischen Ermittlung von Namen und Adressen einschlägiger Lärmforscher/innen, wurden diese um die Zusendung von Fragebögen sowie ergänzender Materialien aus eigenen Lärmwirkungsstudien gebeten. Die zugesandten Fragebögen werden gegebenenfalls übersetzt und mit der Methode der qualitativen Inhaltsanalyse ausgewertet. Hierbei werden die Fragebögen im Hinblick auf formelle Aspekte (z.B. Jahr der Erhebung, Sprache, Art der Befragungsmethode) wie auch im Hinblick auf strukturelle Aspekte (z.B. Umfang des Fragebogens, abgefragte Variablengruppen, Antwortformate) ausgewertet. Hauptgegenstand der Auswertung ist aber insbesondere die Auswertung der Lärmwirkungsvariablen (z.B. die Abfrage der globalen Lärmbelästigung, Aktivitätenstörungen, Kommunikationsstörungen) sowie der Moderatorvariablen (z.B. Lärmempfindlichkeit, Lärmbewältigungsvermögen, misfeasance). Parallel dazu wurde eine Datenbank entwickelt, in der die Ergebnisse der Analysen dargestellt und verwaltet werden. Diese Datenbank wird ab November 2001 im Internet unter http://www.eco.psy.ruhr-uni-bochum.de/nqd für jede/n interessierte/n Forscher/in zugänglich und nutzbar sein. Langfristig ist darüber hinaus geplant, ein Archiv mit den Original-Fragebögen aufzubauen, in dem einzelne Fragebögen auf Wunsch eingesehen werden können.
Subproject 3 will investigate the effect of shifting from continuously flooded rice cropping to crop rotation (including non-flooded systems) and diversified crops on the soil fauna communities and associated ecosystem functions. In both flooded and non-flooded systems, functional groups with a major impact on soil functions will be identified and their response to changing management regimes as well as their re-colonization capability after crop rotation will be quantified. Soil functions corresponding to specific functional groups, i.e. biogenic structural damage of the puddle layer, water loss and nutrient leaching, will be determined by correlating soil fauna data with soil service data of SP4, SP5 and SP7 and with data collected within this subproject (SP3). In addition to the field data acquired directly at the IRRI, microcosm experiments covering the broader range of environmental conditions expected under future climate conditions will be set up to determine the compositional and functional robustness of major components of the local soil fauna. Food webs will be modeled based on the soil animal data available to gain a thorough understanding of i) the factors shaping biological communities in rice cropping systems, and ii) C- and N-flow mediated by soil communities in rice fields. Advanced statistical modeling for quantification of species - environment relationships integrating all data subsets will specify the impact of crop diversification in rice agro-ecosystems on soil biota and on the related ecosystem services.
Prehistoric pits are filled with ancient topsoil material, which has been preserved there over millennia. A characteristic of these pit fillings is that their colour is different depending on the time the soil material was relocated. Soil colour is the result of soil forming processes and soil properties, and it could therefore indicate the soil characteristics present during that specific period. To the best of our knowledge, no investigation analysed and explained the reasons for these soil colour changes over time. The proposed project will investigate soil parameters from pit fillings of different archaeological periods in the loess area of the Lower Rhine Basin (NW-Germany). It aims to implement the measurement of colour spectra as a novel analytical tool for the rapid analyses of a high number of soil samples: the main goal is to relate highresolution colour data measured by a spectrophotometer to soil parameters that were analysed by conventional pedogenic methods and by mid infrared spectroscopy (MIRS), with a main focus on charred organic matter (BPCAs). This tool would enable us to quantify the variation of soil properties over a timescale of several millennia, during different prehistoric periods at regional scale and for loess soils in general. Detailed information concerning changing soil properties on a regional scale is necessary to determine past soil quality and it helps to increase our understanding of prehistoric soil cultivation practices. Furthermore, these information could also help to increase our understanding about agricultural systems in different archaeological periods.
The sorption of anions in geotechnical multibarrier systems of planned high level waste repositories (HLWR) and of non-ionic and organic pollutants in conventional waste disposals are in the center of recent research. In aquatic systems, persistent radionuclides such as 79Se, 99Tc, 129I exist in a form of anions. There is strongly increasing need to find materials with high sorption capacities for such pollutants. Specific requirements on barrier materials are long-term stability of adsorbent under various conditions such as T > 100 C, varying hydrostatic pressure, and the presence of competing ions. Organo-clays are capable to sorb high amounts of cations, anions and non-polar molecules simultaneously having selectivity for certain ions. This project is proposed to improve the understanding of sorption and desorption processes in organo-clays. Additionally, the modification of material properties under varying chemical and thermal conditions will be determined by performing diffusion and advection experiments. Changes by sorption and diffusion will be analyzed by determining surface charge and contact angles. Molecular simulations on models of organo-clays will be conducted in an accord with experiments with aim to understand and analyze experimental results. The computational part of the project will profit from the collaboration of German partner with the group in Vienna, which has a long standing experience in a modeling of clay minerals.
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