Data gathered for a split-clutch experiment on intergenerational response to temperature variation in threespine stickleback (Gasterosteus aculeatus) between May-Nov 2021, at the laboratory in the Wadden Sea Station, Sylt (Germany; 55.020993, 8.437764). Parents were exposed to one of three temperature treatments (constant temperature, natural temperature variation, and increased temperature variation) for 2 months and then bred; clutches were split between the same three temperature treatments and monitored for 90 days post-hatch. Various characteristics were measured to assess the impact and interaction of both parent and offspring temperature variation treatment on offspring growth, survival, and development. Data include standard lengths of parent (n=92) and offspring (n=760) fish (collected using digital measurement of calibrated photographs), timing of breeding events, clutch and egg size, survival of offspring fish over 90 days post-hatch, morphological landmarks for morphometric analysis of offspring fish, and hourly temperatures of treatment conditions.
We investigated effects of temperature during ontogeny of the kelp Laminaria digitata across haploid gametophyte and diploid sporophyte life cycle stages in 5 distinct genetic lines. We sampled meristematic discs from wild sporophytes on the island of Helgoland (North Sea; 54.1779 N, 7.8926 E) in May 2017, July 2017 and February 2018 and conducted a seasonal growth experiment at 5°C and 15°C over 14 days (experiment 1). Based on meiospores from five individuals (genetic lines) sampled in July 2017, we applied a full-factorial experimental design to generate different temperature histories by applying 5°C and 15°C during meiospore germination, gametogenesis of parental gametophytes and recruitment of offspring sporophytes (19-26 days; recruitment), and juvenile sporophyte rearing (91-122 days). We then tested for thermal plasticity among temperature history treatments at 5°C and 15°C in a final 12-day experiment assessing growth, the storage compound mannitol, carbon and nitrogen contents, and fluorometric responses in 3-4 month old sporophytes (experiment 2).